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Full-Text Articles in Dentistry
Effects Of Synthetic Cannabinoid Use, Susan H. Davide
Effects Of Synthetic Cannabinoid Use, Susan H. Davide
Publications and Research
Synthetic Cannabinoid Use: Oral health professionals need to be aware of the systemic and oral health effects of the substances in this class of drugs.
Worldwide Use Of Triclosan: Can Dentistry Do Without This Antimicrobial?, Diana V. Macri
Worldwide Use Of Triclosan: Can Dentistry Do Without This Antimicrobial?, Diana V. Macri
Publications and Research
No abstract provided.
Proteomic Approach For Extracting Cytoplasmic Proteins From Streptococcus Sanguinis Using Mass Spectrometry, Fadi Elrami, Kristina Nelson, Ping Xu
Proteomic Approach For Extracting Cytoplasmic Proteins From Streptococcus Sanguinis Using Mass Spectrometry, Fadi Elrami, Kristina Nelson, Ping Xu
Philips Institute for Oral Health Research Publications
Streptococcus sanguinis is a commensal and early colonizer of oral cavity as well as an opportunistic pathogen of infectious endocarditis. Extracting the soluble proteome of this bacterium provides deep insights about the physiological dynamic changes under different growth and stress conditions, thus defining “proteomic signatures” as targets for therapeutic intervention. In this protocol, we describe an experimentally verified approach to extract maximal cytoplasmic proteins from Streptococcus sanguinis SK36 strain. A combination of procedures was adopted that broke the thick cell wall barrier and minimized denaturation of the intracellular proteome, using optimized buffers and a sonication step. Extracted proteome was quantitated …