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Random Mutagenesis Of The Aspergillus Oryzae Genome Results In Fungal Antibacterial Activity, Cory A. Leonard, Stacy D. Brown, James Russell Hayman
Random Mutagenesis Of The Aspergillus Oryzae Genome Results In Fungal Antibacterial Activity, Cory A. Leonard, Stacy D. Brown, James Russell Hayman
ETSU Faculty Works
Multidrug-resistant bacteria cause severe infections in hospitals and communities. Development of new drugs to combat resistant microorganisms is needed. Natural products of microbial origin are the source of most currently available antibiotics. We hypothesized that random mutagenesis of Aspergillus oryzae would result in secretion of antibacterial compounds. To address this hypothesis, we developed a screen to identify individual A. oryzae mutants that inhibit the growth of Methicillin-resistant Staphylococcus aureus (MRSA) in vitro. To randomly generate A. oryzae mutant strains, spores were treated with ethyl methanesulfonate (EMS). Over 3000 EMS-treated A. oryzae cultures were tested in the screen, and one isolate, …
Stability Of Extemporaneously Prepared Lansoprazole Suspension At Two Temperatures, Jordan T. Morrison, Ralph A. Lugo, Jim C. Thigpen, Stacy D. Brown
Stability Of Extemporaneously Prepared Lansoprazole Suspension At Two Temperatures, Jordan T. Morrison, Ralph A. Lugo, Jim C. Thigpen, Stacy D. Brown
ETSU Faculty Works
OBJECTIVE The purpose of this study was to examine the stability of a generic lansoprazole product in a 3 mg/mL sodium bicarbonate suspension under room temperature and refrigerated conditions. METHODS Lansoprazole suspensions (3 mg/mL) were prepared in triplicate using an 8.4% sodium bicarbonate vehicle for each storage condition (room temperature and refrigerated). During 1 month, samples from each replicate were periodically removed and analyzed for lansoprazole concentration by liquid chromatography–tandem mass spectrometry (LC-MS/MS). Each sample was spiked with 10 mg/L omeprazole to serve as the internal standard. A positive electrospray LC-MS/MS method was validated over the calibration range of 5 …