Chemicals and Drugs Commons^™

Specific Increase In Mdr1 Mediated Drug-Efflux In Human Brain Endothelial Cells Following Co-Exposure To Hiv-1 And Saquinavir, Upal Roy, Christine Bulot, Kerstin Honer Zu Bentrup, Debasis Mondal

HWCOM Faculty Publications

Persistence of HIV-1 reservoirs within the Central Nervous System (CNS) remains a significant challenge to the efficacy of potent anti-HIV-1 drugs. The primary human Brain Microvascular Endothelial Cells (HBMVEC) constitutes the Blood Brain Barrier (BBB) which interferes with anti-HIV drug delivery into the CNS. The ATP binding cassette (ABC) transporters expressed on HBMVEC can efflux HIV-1 protease inhibitors (HPI), enabling the persistence of HIV-1 in CNS. Constitutive low level expression of several ABC-transporters, such as MDR1 (a.k.a. P-gp) and MRPs are documented in HBMVEC. Although it is recognized that inflammatory cytokines and exposure to xenobiotic drug substrates (e.g HPI) can …

Hiv Infection And Drugs Of Abuse: Role Of Acute Phase Proteins, Thangavel Samikkannu, Kurapti Vk Rao, Adriana Y. Arias, Aarthi Kalaichezian, Vidya Sagar, Changwon Yoo, Madhavan Pn Nair

HWCOM Faculty Publications

Background

HIV infection and drugs of abuse such as methamphetamine (METH), cocaine, and alcohol use have been identified as risk factors for triggering inflammation. Acute phase proteins such as C-reactive protein (CRP) and serum amyloid A (SAA) are the biomarkers of inflammation. Hence, the interactive effect of drugs of abuse with acute phase proteins in HIV-positive subjects was investigated.

Methods

Plasma samples were utilized from 75 subjects with METH use, cocaine use, alcohol use, and HIV-positive alone and HIV-positive METH, cocaine, and alcohol users, and age-matched control subjects. The plasma CRP and SAA levels were measured by ELISA and western …