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Assessment Of Lipid Extraction From Human Bone On Subsequent Demineralization, Jaeeun Lee
Assessment Of Lipid Extraction From Human Bone On Subsequent Demineralization, Jaeeun Lee
Biological Sciences Theses & Dissertations
The purpose of this study was to assess the effectiveness of various solvents m removal of lipids from human bone, and to determine the effects of lipid extraction on subsequent acid solubilization of the mineral components of bone. Specifically, the following processing steps were studied; 1) ground human bone was de fatted in various ethanol / water mixtures, and / or chloroform / methanol ( 2: 1, v:v ), 2) ground human bone which had lipids extracted ( in absolute ethanol ) and bone which had not had lipids extracted were demineralized using vanous concentrations of hydrochloric acid to assess …