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Functionalization Of Peptide Nucleic Acids Via Post-Synthetic Click Chemistry, Xiaoxiao Wang
Functionalization Of Peptide Nucleic Acids Via Post-Synthetic Click Chemistry, Xiaoxiao Wang
Electronic Thesis and Dissertation Repository
Peptide Nucleic Acid (PNA) has shown great potential in molecular diagnostics, antisense/antigene therapy and nanotechnology. Like other synthetic nucleic acids and artificial analogues, PNA has been extensively modified to achieve better performance in these applications. To efficiently develop PNA probes for molecular diagnosis, this thesis is focused on versatile functionalization of PNA via post-synthetic click chemistry.
Chapter 2 presents the synthesis of quencher-free PNA molecular beacons (MBs) targeting a cystic fibrosis transmembrane conductance regulator (CFTR) sequence mutation. To avoid the tedious synthesis of functionalized PNA monomers for probe development, a simple approach to modify PNA oligomers by post-synthetic on-resin click …