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Full-Text Articles in Chemicals and Drugs
Repurposing The Calcium Biosensor Twitch-2b: Creating Transition Metal Detection Proteins Through Fluorescent Analysis, Sarah Mateer
Repurposing The Calcium Biosensor Twitch-2b: Creating Transition Metal Detection Proteins Through Fluorescent Analysis, Sarah Mateer
Honors Projects and Presentations: Undergraduate
Metal ions are key in regulating many biological functions, but their accumulation is associated with neurological diseases. Cellular metal ion concentrations are often detected through exogenous fluorescent dyes, but less invasive and more specific detection can be done using genetically encoded FRET-enabled metal-binding proteins, such as Twitch-2B. Twitch-2B and other ratiometric proteins change conformation when a calcium ion is bound to the active site, initiating an energy transfer between two fluorescent peptides that can be quantified through fluorescent spectroscopy. Previous analyses have demonstrated that wild-type calcium binding can be accurately detected via this method for concentrations from 10 uM to …
Fluorescently Labeled Sirnas And Their Theranostic Applications In Cancer Gene Therapy, Stephen David Kozuch
Fluorescently Labeled Sirnas And Their Theranostic Applications In Cancer Gene Therapy, Stephen David Kozuch
Seton Hall University Dissertations and Theses (ETDs)
Gene therapy has emerged as a promising precision nano-medicine strategy in the treatment of numerous diseases including cancer. At the forefront of its utility are the applications of short-interfering RNA (siRNA), that silence oncogenic mRNA expression leading to cancer cell death through the RNA interference (RNAi) pathway. Despite the therapeutic potential, siRNAs are limited by poor pharmacological properties, which has hindered their translation into the clinic. Recent studies, however, have highlighted the applications of modified siRNAs, including the use of fluorescent probes and siRNA nanostructures in cancer detection and treatment. The siRNAs reported in this thesis are designed to target …