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Full-Text Articles in Chemicals and Drugs
Determination Of Nuclear Factor-Kappa B Activation In Cultured Renal Epithelial Cells And Cardiac Myocytes Exposed To Cocaine And Morphine, David Angeles Tiangco
Determination Of Nuclear Factor-Kappa B Activation In Cultured Renal Epithelial Cells And Cardiac Myocytes Exposed To Cocaine And Morphine, David Angeles Tiangco
Biological Sciences Theses & Dissertations
Nuclear factor-kappa B (NF-kB) is an important transcription factor that participates in the genetic regulation of inflammation in many tissues. Cocaine and heroin are drugs of abuse associated with renal and cardiac pathology. The purpose of this study was to test the hypothesis that exposure to cocaine or morphine (a metabolite of heroin) would activate NF-kB in renal epithelial cells (COS-7) and cardiac myocytes (H9c2) grown in culture. COS-7 and H9c2 cells were co-transfected with an experimental reporter specific for NF-kB activation and a control reporter with constitutive activity. A dual-luciferase assay was used to determine levels of NF-kB activation …
A Mechanistic Study Of Myoglobin Nephrotoxicity, Jennifer L. Minigh
A Mechanistic Study Of Myoglobin Nephrotoxicity, Jennifer L. Minigh
Theses, Dissertations and Capstones
Myoglobin is an endogenous protein that can become nephrotoxic under certain conditions such as crush injuries, drug overdose, and seizures where prolonged contraction of muscle leads to cell death and leakage of myoglobin. The mechanism of myoglobin-induced nephrotoxicity is not fully understood. The purpose of this study was to characterize the sequence and mechanistic events associated with the in vitro toxicity of myoglobin in renal cortical slices. Renal tissue was isolated from Fischer 344 rats. Slices of renal cortex were prepared by freehand. These slices were then incubated for 60-180 minutes with myoglobin (0-12 mg/mL) pretreated with 4 mM ascorbic …