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- Acquired enamel pellicle (1)
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- Disassembly (1)
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Articles 1 - 3 of 3
Full-Text Articles in Chemicals and Drugs
Protein Detection And Structural Characterization By Mass Spectrometry Using Supramolecular Assemblies And Small Molecules, Bo Zhao
Doctoral Dissertations
Mass spectrometry (MS) has played an increasingly prominent role in proteomics and structure biology because it shows superior capabilities in identification, quantification and structural characterization of proteins. To realize its full potential in protein analysis, significant progress has been made in developing innovative techniques and reagents that can couple to MS detection. This dissertation demonstrates the use of polymeric supramolecular assemblies for enhanced protein detection in complex biological mixtures by MS. An amphiphilic random co-polymer scaffold is developed to form functional supramolecular assemblies for protein/ peptide enrichment. The influences of charge density and functional group pKa on host-guest interactions …
Developing A Dissociative Nanocontainer For Peptide Drug Delivery, Michael Patrick Kelly
Developing A Dissociative Nanocontainer For Peptide Drug Delivery, Michael Patrick Kelly
Dissertations, Theses, and Capstone Projects
The potency and specificity of bioactive peptides have propelled these agents to the forefront of pharmacological research. However, delivery of peptides to their molecular target in cells is a major obstacle to their widespread application. A Trojan Horse strategy of packaging a bioactive peptide within a modified protein cage to protect it during transport, and releasing it at the target site, is a promising delivery method. Recent work has demonstrated that the viral capsid of the P22 bacteriophage can be loaded with an arbitrary, genetically-encoded peptide, and externally decorated with a cell-penetrating peptide, such as HIV-Tat, to translocate across in …
Effect Of Histatin- And Statherin-Derived Engineered Salivary Peptides On Streptococcus Mutans Adhesion And On Enamel Demineralization Provoked By Cariogenic Biofilms, Lina Maria Marin
Electronic Thesis and Dissertation Repository
Dental biofilm is formed onto dental surfaces covered by a layer of specific salivary proteins and peptides named acquired enamel pellicle (AEP). It was previously demonstrated that the statherin- and histatin-derived engineered salivary peptides DR9-DR9 and DR9-RR14 were able to reduce enamel demineralization and displayed antimicrobial activity against Streptococcus mutans, respectively. However, these studies were carried under experimental conditions that did not mimic caries development induced by biofilm and sucrose exposure, the most cariogenic dietary sugar. In this thesis we assessed the effect of the engineered salivary peptides on 1) the adherence of S. mutans to hydroxyapatite (HAp), and …