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Full-Text Articles in Chemicals and Drugs
Effect Of Cocaine On Rabbit Renin Angiotensin System: Cocaine Detection In Adult And Fetal Tissue And Adult Plasma Using Ion Mobility Spectrometry, Barbara Belinda Guinn
Effect Of Cocaine On Rabbit Renin Angiotensin System: Cocaine Detection In Adult And Fetal Tissue And Adult Plasma Using Ion Mobility Spectrometry, Barbara Belinda Guinn
Biological Sciences Theses & Dissertations
Ion mobility spectrometry (IMS) was used to detect cocaine and/or the metabolite ecgonine methyl ester (EME) in tissue and plasma samples from male, maternal, and fetal New Zealand White (NZW) rabbits. EME was consistently detected in all adult plasma samples 5 minutes after the injection of cocaine hydrochloride (cocaine•HCI, 2 mg/kg). EME was also in the liver, kidneys, testicles, and ovaries. Five minutes after the injection of cocaine•HCI, the male arterial pressure (MAP) increased from a mean control value of 79 ± 3.2 mmHg to 88 ± 4.1 mmHg, and the pCO2 increased from a control value of28 ± …
Isolation And Partial Characterization Of Basic, Sperm-Specific, Nuclear Proteins In The Dog And Hampster, Richard William Hall
Isolation And Partial Characterization Of Basic, Sperm-Specific, Nuclear Proteins In The Dog And Hampster, Richard William Hall
Biological Sciences Theses & Dissertations
The purpose was to determine whether dogs and hamsters contain the same sperm-specific protamine and testis specific proteins (TP) as other eutherian mammals previously studied. The proteins (protamine and TPs) were isolated from spermatids and mature spermatozoa, and were subjected to electrophoresis in an acid-urea, polyacrylamide gel system according to the procedure of Panyim and Chalkley (1962). The results indicated that, regardless of the anatomical location of sperm acquisition, acid precipitation technique, or denaturing agents used, epididymal and testicular samples from both the dog and hamster contained protamine in elongated spermatids and mature spermatozoa. The protamines from dogs and hamsters …
Evaluation Of Peptide Assimilation In Ehrlich Ascites Tumor Cells, John M. Castellano
Evaluation Of Peptide Assimilation In Ehrlich Ascites Tumor Cells, John M. Castellano
Biological Sciences Theses & Dissertations
The dipeptides glycyl-L-(3H)-leucine and L-phenylalanyl-L-(3H)-leucine were synthesized in radioactive form and utilized in the analysis of peptide transport and hydrolysis by Ehrlich ascites tumor cells. The tumor cells were cultured in the abdominal cavity of CF-1 albino mice and harvested into sterile isotonic buffer for all experimental analyses.
The results of this study reveal that the tumor cells rapidly accumulate radiolabel when incubated with the above mentioned peptides, but that the peptide is most probably hydrolyzed extracellularly and free L-(3)-leucine transported. This hypothesis is based on the observations of reduction in accumulation of radiolabel …