Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons^™

Durand Et Al 2012 Supplemental Figures, M. Durand, Svetlana V. Komarova, Ajay Bhargava, Keying Li, Cara Fiorino, Osama Maria, Noushin Nabavi, Morris Manolson, Rene Harrison, S. Jeffrey Dixon, Stephen Sims, Marcin Mizianty, Lukasz Kurgan, Sonia Haroun, Gilles Boire, Maria Lucena-Fernandes, Artur De Brum-Fernandes

Physiology and Pharmacology Publications

Objective. Our objective was to compare the osteoclastogenic capacity of peripheral blood mononuclear cells (PBMCs) from patients with osteoarthritis (OA) to that of PBMCs from self-reported normal individuals.

Methods. PBMCs from 140 patients with OA and 45 healthy donors were assayed for CD14⁺ expression and induced to differentiate into osteoclasts (OCs) over 3 weeks in vitro. We assessed the number of the OCs, their resorptive activity, OC apoptosis, and expression of the following cytokine receptors: receptor activator of nuclear factor κB (RANK), interleukin-1 receptor type I (IL-1R1) and IL-1R2. A ridge logistic regression classifier was developed …