Open Access. Powered by Scholars. Published by Universities.®
Alternative and Complementary Medicine Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Alternative and Complementary Medicine
Analysis Of Corrinoids In Ovine Tissues, R Kelly, Tini Gruner, J Furlong, A Sykes
Analysis Of Corrinoids In Ovine Tissues, R Kelly, Tini Gruner, J Furlong, A Sykes
Dr Tini M Gruner
Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids - the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues - in these tissues. Samples were taken from either cobalt-deficient or cobalt-replete ewes, and ruminant and pre-ruminant lambs. In liver, ado-cbl predominated, followed by analogues, OH-cbl and me-cbl. Supplementation with either cobalt (ruminant) or vitamin B12 injections (pre-ruminant) increased the amount of ado-cbl and decreased analogues. In blood, OH-cbl predominated, followed by …