Medicine and Health Sciences Commons^™

Helicobacter Pylori And Intestinal Parasites Co-Infection: Estimation Of Risk Factors Among Dyspeptic Patients In Mukalla City, Hadhramout, Yemen, Eidha Ali Bin-Hameed Bin-Hameed, Huda Mohammed Barajash Barajash

Hadhramout University Journal of Natural & Applied Sciences

The prevalence of Helicobacter pylori (H. pylori) infection among dyspeptic patients was common in developing countries. H. pylori is capable of colonizing the gastric mucosa, and the infections often lead to the development of various gastrointestinal complaints. Human colonization by H. pylori and intestinal parasites is also common. This study aimed to estimate the prevalence co-infection of H. pylori and intestinal parasites and associated risk factors among dyspeptic patients referred to Mukalla city hospitals, Hadhramout, Yemen. In this cross-sectional study, one hundred stool samples were collected and diagnosed for detection H. pylori infection by rapid immunochromatographic stool-based antigen …

Risk Factors Of Clostridium Difficile Infection In Helicobacter Pylori Diagnosed Patients: A Multicenter Study, Samiksha Pandey, Tamera Tennant, Ashbina Pokharel, Andrew Aneese, Alexandra Halalau

Posters

• Clostridium difficile infection (CDI) is one of the most common gastrointestinal illnesses. • There are conflicting studies regarding the association of CDI with Helicobacter Pylori (H. pylori) treatment regimens.1-3 • Our aim is to investigate the risk of CDI in those treated for H. pylori as well as characterize other risk factors for developing CDI.

Characterization Of Gut Microbiome And Metabolome In Helicobacter Pylori Patients In An Underprivileged Community In The United States, Brian White, John Sterrett, Zoya Grigoryan, Lauren Lally, Jared Heinze, Hyder Alikhan, Christopher Lowry, Lark Perez, Joshua Desipio, Sangita Phadtare

Division of Internal Medicine Faculty Papers & Presentations

BACKGROUND Helicobacter pylori (H. pylori), a bacterium that infects approximately half of the world's population, is associated with various gastrointestinal diseases, including peptic ulcers, non-ulcer dyspepsia, gastric adenocarcinoma, and gastric lymphoma. As the burden of antibiotic resistance increases, the need for new adjunct therapies designed to facilitate H. pylori eradication and reduce negative distal outcomes associated with infection has become more pressing. Characterization of the interactions between H. pylori, the fecal microbiome, and fecal fatty acid metabolism, as well as the mechanisms underlying these interactions, may offer new therapeutic approaches.

AIM To characterize the gut microbiome and metabolome in H. …

Characterization Of Gut Microbiome And Metabolome In Helicobacter Pylori Patients In An Underprivileged Community In The United States, Brian White, John D Sterrett, Zoya Grigoryan, Lauren Lally, Jared D Heinze, Hyder Alikhan, Christopher A Lowry, Lark Perez, Joshua Desipio, Sangita Phadtare

Faculty Scholarship for the College of Science & Mathematics

Background: Helicobacter pylori (H. pylori), a bacterium that infects approximately half of the world's population, is associated with various gastrointestinal diseases, including peptic ulcers, non-ulcer dyspepsia, gastric adenocarcinoma, and gastric lymphoma. As the burden of antibiotic resistance increases, the need for new adjunct therapies designed to facilitate H. pylori eradication and reduce negative distal outcomes associated with infection has become more pressing. Characterization of the interactions between H. pylori, the fecal microbiome, and fecal fatty acid metabolism, as well as the mechanisms underlying these interactions, may offer new therapeutic approaches. Aim: To characterize the gut microbiome and metabolome in H. …

Frequency And Risk Factors Of Non-Alcoholic Fatty Liver Disease In Helicobacter Pylori-Infected Dyspeptic Patients: A Cross-Sectional Study, Basit Siddiqui, Muhammad Kamran, Shiyam Sunder Tikmani, Rabeea Azmat, Zain Mushtaq, Saad Bin Zafar Mahmood, Muhammad Tahir Khan, Javed Yakoob, Zaigham Abbas

Section of Gastroenterology

Background: In dyspeptic patients with Helicobacter pylori contributes to non-alcoholic fatty liver disease. However, little evidence available from Pakistan.
Objective: The study aims to determine the frequency and risk factors of non-alcoholic fatty liver disease in dyspeptic patients with Helicobacter pylori.
Methods: This cross-sectional study was conducted between 22 November 2016 and 30 June 2018. Adults of age between 18 and 90 years who attended the out-patient department due to abdominal discomfort, pain, fullness, and bloating who underwent upper gastrointestinal tract endoscopy were enrolled after taking informed consent. Patients with celiac disease, inflammatory bowel disease, taking alcohol, pregnant women …

Think Twice Before Prescribing Antibiotics For That Swollen Knee: The Influence Of Antibiotics On The Diagnosis Of Periprosthetic Joint Infection., Graham S Goh, Javad Parvizi Md

Rothman Institute Faculty Papers

Periprosthetic joint infection (PJI) is a rare but devastating complication after total joint arthroplasty. An estimated 7-12% of patients have negative cultures despite clear clinical evidence of infection. One oft-cited reason for this occurrence is the administration of antibiotics in the weeks prior to obtaining cultures. This article reviews the influence of antibiotics on the diagnosis of PJI. Specifically, we examine the effect of prophylactic and therapeutic antibiotic administration on the diagnostic accuracy of microbiological cultures as well as serum and synovial biomarkers. We also explore the potential of molecular techniques in overcoming these limitations in patients who have received …

Detection Of Helicobacter Pylori By Invasive Tests In Adult Dyspeptic Patientsand Antibacterial Resistance To Six Antibiotics, Including Rifampicin In Turkey. Isclarithromycin Resistance Rate Decreasing?, Mustafa Akar, Fuat Aydin, Tuba Kayman, Seçi̇l Abay, Emre Karakaya

Turkish Journal of Medical Sciences

Background/aim: The prevalence of Helicobacter pylori is reported to be roughly 80% in Turkey, and only very few culture-based studies are available on antibacterial resistance in adult dyspeptic patients. This study was carried out in adult dyspeptic patients with an aim to: (i) detect H. pylori by invasive tests (culture, polymerase chain reaction, and histopathology) and (ii) determine the current resistance rates of H. pylori isolates to six antibiotics, including rifampicin. Materials and methods: This study was conducted in 422 adult dyspeptic patients. The presence of H. pylori was demonstrated by culture, polymerase chain reaction, and the histopathology of gastric …

Immunostimulatory Membrane Proteins Potentiate H. Pylori-Induced Carcinogenesis By Enabling Caga Translocation, Matthew G. Varga, Cecily R. Wood, Julia Butt, Mackenzie E. Ryan, Wei-Cheng You, Kaifeng Pan, Tim Waterboer, Meira Epplein, Carrie L. Shaffer

Veterinary Science Faculty Publications

Infection with Helicobacter pylori is the single greatest risk factor for developing gastric adenocarcinoma. In prospective, population-based studies, seropositivity to the uncharacterized H. pylori proteins Hp0305 and Hp1564 was significantly associated with cancer risk in East Asia. However, the mechanism underlying this observation has not been elucidated. Here, we show that Hp0305 and Hp1564 act in concert with previously ascribed H. pylori virulence mechanisms to orchestrate cellular alterations that promote gastric carcinogenesis. In samples from 546 patients exhibiting premalignant gastric lesions, seropositivity to Hp0305 and Hp1564 was significantly associated with increased gastric atrophy across all stomach conditions. In vitro, …

Relationship Between Helicobacter Pylori Infection And Pityriasis Versicolor:Can Helicobacter Pylori Infection Be A New Etiologic Factor For Pityriasis Versicolor?, Ömer Kutlu, Zeynal Doğan, Hati̇ce Meral Ekşi̇oğlu, Murat Keki̇lli̇

Turkish Journal of Medical Sciences

Background/aim: H. pylori has been found to be related to certain dermatological diseases. However, there is no data as yet to propose an association between H. pylori and pityriasis versicolor. In this study, we aimed to evaluate the association between H. pylori and pityriasis versicolor. Materials and methods: This was a prospective study performed in the Gastroenterology and Dermatology and Venereology departments of the Health Sciences University, Ankara Training and Research Centre. A total of 57 consecutive patients (27 pityriasis versicolor, 30 telogen effluvium) were enrolled from the Department of Dermatology and Venereology. All patients were screened for H. pylori …

The Discovery And Development Of Thienopyrimidines As Inhibitors Of Helicobacter Pylori, Alex Kagabo Mugengana

Theses and Dissertations (ETD)

The rate of successful treatment for Helicobacter pylori infections, with the clarithromycin triple therapy, is only 75%. The triple therapy, which consists of a proton pump inhibitor and two broad-spectrum antibiotics such as clarithromycin and amoxicillin, is becoming less effective due to the rise of strains with resistance against these antibiotics. In the search for narrow spectrum drugs for the treatment of H. pylori infections, a high-throughput screen was performed to identify selective compounds against H. pylori. This screen revealed two selective and structurally related thienopyrimidines. Structure-activity relationship of the thienopyrimidines against H. pylori was examined through the synthesis of …

Helicobacter Pylori Pathophysiology, Alisha Smith

Nursing Student Class Projects (Formerly MSN)

Abstract

This poster provides current research evidence on Helicobacter Pylori pathophysiology, treatment guidelines, and the potential etiology of associated disorders. The purpose is to provide a detailed overview of the functional changes within the intestinal cells caused by the microorganism. Furthermore, explaining of the mechanism and clinical manifestations of symptoms. The research focuses on risk factors, transmission, diagnostic evaluation, and the implications for nursing care. The best-available evidence offers concrete data examining the detrimental effects of the immune and inflammatory response stimulated by H. pylori. A description of the functional structures of H. pylori and its’ ability to survive. …

Chronic Gastritis And Carcinogenesis Issues, G. N. Sobirova, U. K. Abdullaeva

Central Asian Problems of Modern Science and Education

In the review risk factors which transformations chronic atrophic a gastritis in a stomach cancer promote are analyzed. The characteristic of precancer conditions as increase in risk of development of a cancer of a stomach is given. As risk factor of a cancer of a stomach definition of subtypes intestinal metaplasia is offered, dividing on full and incomplete taking into account decrease in an expression gastric mucin MUC1, MUC5AC and MUC6. The role of a genetic susceptibility of an organism to infected Н pylori, the factors of its pathogenicity promoting metaplasia epithelium is analyzed. Also role Toll-like of receptors 4 …

Assessment And Analysis Of H.Pylori Infection Treatment Strategies Of St. Vincent Hospital's Family And Internal Medicine Clinics, Rebecca Orr

Undergraduate Honors Thesis Collection

Helicobacter pylori is a gram-negative bacteria that is responsible for causing chronic gastritis, ulcers in the stomach and intestine, and eventually even gastric lymphoma or cancer. Multiple treatment options have been recommended for treating an H. pylori infection based on kidney function, previous antibiotic exposure, and whether or not the infection is recurrent. The most common regimens used at St. Vincent's primary care centers closely mimic the American College of Gastroenterology's clinical guidelines. However, these medications (clarithromycin and levofloxacin) have been associated with high rates of resistance in other countries. The United States has very limited data on H. pylori's …

Effects Of Ankaferd Hemostat On Helicobacter Pylori Strains And Antibiotic Resistance, Rafi̇ye Çi̇ftçi̇ler, Ahmet Koluman, İbrahi̇m Celaletti̇n Haznedaroğlu, Nejat Akar

Turkish Journal of Medical Sciences

Background/aim: Ankaferd hemostat (ABS; Ankaferd blood stopper, İstanbul, Turkey) is a folkloric medicinal plant extract. The aim of this study was to determine the effect of Ankaferd hemostat (ABS) on the fate of Helicobacter pylori strains. The study also aims to determine alterations in the antimicrobial resistance of three different H. pylori strains in response to ABS exposure.Materials and methods: H. pylori Strain 1 was obtained from the culture collection ATCC 43504 and passaged three times for viability. Strain 2 was isolated from a gastric ulcer patient and Strain 3 was isolated from a gastritis patient. 1% of ABS was …

Elucidating The Importance Of Hope And Its Potential Lewis Glycosylation In Helicobacter Pylori, Keertika Yogendirarajah

Electronic Thesis and Dissertation Repository

Helicobacter pylori colonizes 50% of the world’s population, whereby glycoproteins and Lewis Y-containing lipopolysaccharides contribute to its pathogenesis. We investigated whether the HopE porin is glycosylated, if the glycan is Lewis Y, and if this is mediated by the putative oligosaccharide transferase HP0946 or the O-antigen ligase WaaL. Western blotting was performed on outer membranes with anti-HopE antibodies, anti-Lewis Y antibodies and fucose-binding BambL lectin to ascertain HopE glycosylation. We discovered that HopE is likely glycosylated by a non-Lewis Y fucose-containing glycan and neither HP0946 nor WaaL are the transferase. Additionally, we investigated HopE’s role in antibiotic susceptibility via Etest …

Epigenetic Regulation Of Tumor Suppressors By Helicobacter Pylori Enhances Ebv-Induced Proliferation Of Gastric Epithelial Cells, Saurabh Pandey, Hem Chandra Jha, Sanket Kumar Shukla, Meghan K. Shirley, Erle S. Robertson

Department of Medicine Faculty Papers

Helicobacter pylori and Epstein-Barr virus (EBV) are two well-known contributors to cancer and can establish lifelong persistent infection in the host. This leads to chronic inflammation, which also contributes to development of cancer. Association with H. pylori increases the risk of gastric carcinoma, and coexistence with EBV enhances proliferation of infected cells. Further, H. pylori-EBV coinfection causes chronic inflammation in pediatric patients. We have established an H. pylori-EBV coinfection model system using human gastric epithelial cells. We showed that H. pylori infection can increase the oncogenic phenotype of EBV-infected cells and that the cytotoxin-associated gene (CagA) protein encoded by H. …

In Vivo Structures Of The Helicobacter Pylori Cag Type Iv Secretion System, Yi-Wei Chang, Carrie L. Shaffer, Lee A. Rettberg, Debnath Ghosal, Grant J. Jensen

Veterinary Science Faculty Publications

The type IV secretion system (T4SS) is a versatile nanomachine that translocates diverse effector molecules between microbes and into eukaryotic cells. Here, using electron cryotomography, we reveal the molecular architecture of the Helicobacter pylori cag T4SS. Although most components are unique to H. pylori, the cag T4SS exhibits remarkable architectural similarity to other T4SSs. Our images revealed that, when H. pylori encounters host cells, the bacterium elaborates membranous tubes perforated by lateral ports. Sub-tomogram averaging of the cag T4SS machinery revealed periplasmic densities associated with the outer membrane, a central stalk, and peripheral wing-like densities. Additionally, we resolved pilus-like …

Genetic Signatures For Helicobacter Pylori Strains Of West African Origin, Kennady K. Bullock, Carrie L. Shaffer, Andrew W. Brooks, Ousman Secka, Mark H. Forsyth, Mark S. Mcclain, Timothy L. Cover

Veterinary Science Faculty Publications

Helicobacter pylori is a genetically diverse bacterial species that colonizes the stomach in about half of the human population. Most persons colonized by H. pylori remain asymptomatic, but the presence of this organism is a risk factor for gastric cancer. Multiple populations and subpopulations of H. pylori with distinct geographic distributions are recognized. Genetic differences among these populations might be a factor underlying geographic variation in gastric cancer incidence. Relatively little is known about the genomic features of African H. pylori strains compared to other populations of strains. In this study, we first analyzed the genomes of …

Current Status Of Helicobacter Pylori Association With Haematological And Cardiovascular Diseases: A Mini Review, Jibran Sualeh Muhammad, Syed Faisal Zaidi, Sheikh Abdul Saeed, Muhammad Ishaq

Department of Biological & Biomedical Sciences

Helicobacter pylori infection is considered the most commonly prevalent gastrointestinal pathogen where it manages to survive despite the hostile environment of human stomach, leading to various gastric diseases including gastric cancer. Due to the chronic inflammatory state induced by H. pylori and its interaction with host immune system have diverted researchers to investigate its correlation with systemic diseases outside of the gastrointestinal tract. This literature review was done to explore the association of H. pylori infection with haematological and cardiovascular diseases. We used medical subject heading (MeSH) terms "Helicobacter pylori" with "inflammation," "haematological diseases," "coronary heart diseases" or "vascular diseases" …

Low Rate Of Recurrence Of Helicobacter Pylori Infection In Spite Of High Clarithromycin Resistance In Pakistan, Javed Yakoob, Shahab Abid, Wasim Jafri, Zaigham Abbas, Khalid Mumtaz, Saeed Hamid, Rashida Ahmed

Rashida Ahmed

Background The aim was to investigate the reinfection rate of H. pylori during a follow-up period of 12 months in adults who had undergone eradication therapy. Methods One hundred-twenty patients; 116 with gastritis, 3 with duodenal ulcer and 1 gastric ulcer, were studied. Their mean age was 41 ± 13 years (range 18–77) and male: female ratio of 2:1. H. pylori were cultured and antibiotic sensitivity was determined by Epsilometer test (E-test) for clarithromycin (CLR) and amoxicillin (AMX). Primers of urease C gene of H. pylori and Sau-3 and Hha I restriction enzymes were used for polymerase chain reaction-restriction fragment …

Prevalence And Risk Factors For Helicobacter Pylori Infection In Southwest China: A Study Of Health Examination Participants Based On 13c-Urea Breath Test, Jie Liu, Yonghong Wang, Qinghua Zhao, Rong Luo, Mingzhao Xiao, Mingjun Zhang, Weibo Xie

Turkish Journal of Medical Sciences

Background/aim: Helicobacter pylori (H. pylori) has a high prevalence in developing countries. We aimed to investigate the current prevalence of H. pylori, as well as its potential serum risk factors, in a population from southwest China.Materials and methods: This cross-sectional study included 10,912 subjects who received medical examinations at the First Affiliated Hospital of Chongqing Medical University in 2014. Data regarding physical examinations and biochemical measurements were collected, and H. pylori infection was diagnosed with a 13C-urea breath test. Logistic regression was conducted to identify the risk factors for H. pylori infection.Results: The infection rate of H. pylori was 34.4% …

Efficient Production Of Nanobodies Against Urease Activity Ofhelicobacter Pylori In Pichia Pastoris, Shahrbanoo Pourasadi, Seyed Latif Mousavi Gargari, Masoumeh Rajabibazl, Shahram Nazarian

Turkish Journal of Medical Sciences

Background/aim: Helicobacter pylori is a major health problem. One of the therapeutic approaches is administration of antibody against H. pylori. The methylotrophic Pichia pastoris is a suitable host for expression of recombinant antibody fragments. The aims of this study were the expression and the evaluation of camelid nanobody in the yeast Pichia pastoris. Materials and methods: The camelid-derived heavy-chain antibody (nanobody) against the UreC subunit of urease from H. pylori was subcloned in the pPink-HC shuttle vector and transferred into Escherichia coli TOP10. After digestion and purification, the shuttle vector was transformed in the PichiaPink expression system. The expression was …

Comparative Analysis Of Selected Cagpai Genes And Different Vaca Genotypes In Iranian And Turkish H. Pylori-Positive Patients Suffering From Gastric Adenocarcinoma And Active Chronic Gastritis, Ali Bahadori, Mohammad Hossein Somi, Fi̇gen Doran, Amir Taher Eftekharsadat, Suna Kizilyildirim, Mahsa Esmaeillou, Maryam Yassi, Başak Bedi̇r, Fati̇h Köksal

Turkish Journal of Medical Sciences

Background/aim: Helicobacter pylori is a pathogen that colonizes a majority of the world's population. Genetic diversity within the virulence genes of bacteria such as cagPAI and vacA may have a modified effect on the pathogenic potential of the bacteria. This study aimed to investigate which genes can be suggested as potentially related virulence factors for H. pylori-associated active chronic gastritis and stomach adenocarcinoma in the northwest of Iran and south of Turkey. Materials and methods: Formalin-fixed, paraffin-embedded stomach biopsy tissue samples were obtained from Iranian and Turkish patients from selected geographical regions. The prevalence of selected cagPAI genes and vacA …

The Role Of Outer Inflammatory Protein A (Oipa) In Vaccination Of Thec57bl/6 Mouse Model Infected By Helicobacter Pylori, Mohaddese Mahboubi, Tahereh Falsafi, Majid Sadeghizadeh, Fatemeh Mahjoub

Turkish Journal of Medical Sciences

Background/aim: Outer inflammatory protein A (OipA) is an important adhesin of Helicobacter pylori. Our goal was to assess the role of OipA in protection of C57BL/6 mice against H. pylori. Materials and methods: C57BL/6 mice were mucosally immunized with recombinant OipA protein, OipA + propolis, propolis, and phosphate-buffered saline. After vaccination, anti-OipA IgA was measured. Mice were challenged three times with 5 x 107 CFU of the H. pylori B19 strain. Two weeks later, bacterial colonization and inflammation in the stomach was analyzed using standard methods. Results: The CFU number in the OipA group was significantly (P < 0.05) lower than that of the control. The CFU number in the OipA + propolis group was higher than those of the OipA and propolis groups. IgA titers were significantly higher (P < 0.0001) in the OipA group compared to the control and OipA + propolis groups. Propolis did not play an adjuvant effect but it interfered with the efficient vaccine effect of OipA. Conclusion: Results show the effect of vaccination by OipA in protection of the mouse model and the importance of OipA in H. pylori pathogenesis. OipA may be proposed as a suitable oral vaccine candidate against H. pylori infection; however, further study is required to determine adjuvant or adverse effects of propolis toward OipA.

Helicobacter Pylori: Types Of Diseases, Diagnosis, Treatment And Causes Of Therapeutic Failure, Cosmin Vasile Obleaga, Cristin Constantin Vere, Ionica Daniel Valcea, Mihai Calin Ciorbagiu, Emil Moraru, Cecil Sorin Mirea

Journal of Mind and Medical Sciences

Acute upper gastrointestinal lesions have a multifactorial etiology but, regardless of the cause, they are related to mucosal barrier destruction. Since Helicobacter pylori induces a superficial chronic gastritis with the infiltration of neutrophils in the mucosa, it was speculated that Helicobacter pylori infection could also cause bleeding lesions. The diagnosis, the proper treatment and the revaluation of its effectiveness actually represent the prophylaxis of some diseases such as peptic ulcer, gastric lymphoma or mucosa-associated lymphoid tissue (MALT) and gastric cancer. These diseases and their severe complications are life-threatening for the patient. Periodic renewal of the treatment and knowing the real …

Peptidomimetic Small Molecules Disrupt Type Iv Secretion System Activity In Diverse Bacterial Pathogens, Carrie L. Shaffer, James A.D. Good, Santosh Kumar, K. Syam Krishnan, Jennifer A. Gaddy, John T. Loh, Joseph Chappell, Fredrik Almqvist, Timothy L. Cover, Maria Hadjifrangiskou

Pharmaceutical Sciences Faculty Publications

Bacteria utilize complex type IV secretion systems (T4SSs) to translocate diverse effector proteins or DNA into target cells. Despite the importance of T4SSs in bacterial pathogenesis, the mechanism by which these translocation machineries deliver cargo across the bacterial envelope remains poorly understood, and very few studies have investigated the use of synthetic molecules to disrupt T4SS-mediated transport. Here, we describe two synthetic small molecules (C10 and KSK85) that disrupt T4SS-dependent processes in multiple bacterial pathogens. Helicobacter pylori exploits a pilus appendage associated with the cag T4SS to inject an oncogenic effector protein (CagA) and peptidoglycan into gastric epithelial cells. In …

The Difference In Prevalence Of Helicobacter Pylori Infectionbetween Chinese And Dayak Ethnics With Dyspepsia Syndrome, Willy Brodus Uwan, Ari Fahrial Syam, C. Rinaldi A. Lesmana, C Martin Rumende

Jurnal Penyakit Dalam Indonesia

Introduction. Helicobacter pylori (H. pylori) infection risk is associated with many factors related to host-agent-environment. Ethnicity is one of the host factors which was the most studied factor overseas. The prevalence of H. pylori infection was found higher in certain ethnic such among Chinese. Based on migration and transmission theory, it was suspected that H. pylori infection was transmitted by people migrating from areas with a high prevalence of infection to the destination area. Chinese in West Borneo are originated from South China region where the prevalence of H. pylori infection is high. It is estimated that there are differences …

Detection Of Helicobacter Heilmannii Type Ii And Helicobacter Pylori Indogs And Their Role In The Development Of Gastritis, Si̇nem Ülgen, Sevgi̇ Ergi̇n, Gülbi̇n Şennazli, Utku Bakirel

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to detect the presence of Helicobacter pylori and Helicobacter heilmannii type II in dogs and evaluate the role of Helicobacter spp. İn chronic gastritis. Gastroscopy was performed in a total of 40 dogs (22 symptomatic and 18 asymptomatic) admitted to the small animal clinic of İstanbul University. In this study, the density of spiral bacteria and the degree and type of gastritis by histopathology were determined. Rapid urease tests were performed on gastric biopsy samples, and the species identity was determined using nested polymerase chain reaction to assess for the presence of H. Heilmannii …

Prevalence Of Helicobacter Pylori Caga, Baba2, And Dupa Genotypes Andcorrelation With Clinical Outcome In Malaysian Patients With Dyspepsia, Hussein Ali Osman, Habsah Hasan, Rapeah Suppian, Syed Hassan, Dzulkarnaen Zakaria Andee, Noorizan Abdul Majid, Bin Alwi Zilfalil

Turkish Journal of Medical Sciences

Background/aim: The severity of disease outcome in dyspepsia has been attributed to Helicobacter pylori virulence genes. The aim of this study was to determine the distribution of H. pylori virulence genes (cagA, babA2, and dupA) and to determine whether or not there arises a significant correlation with clinical dyspepsia outcomes. Materials and methods: H. pylori genotypes cagA, babA2, and dupA were identified by polymerase chain reactions from gastric biopsy samples in 105 H. pylori-positive patients. Results: The positive rates for cagA, babA2, and dupA genes in H. pylori dyspeptic patients were 69.5%, 41.0%, and 22.9%, respectively. cagA was more prevalent …

Analysis Of Vaca/Caga Genotypes/Status In Helicobacter Pylori Isolates From Iranian Children And Their Association With Clinical Outcome, Tahereh Falsafi, Afsaneh Khani, Fatemeh Mahjoub, Ezat Asgarani, Nazli Sotoudeh

Turkish Journal of Medical Sciences

More than 50% of Iranian children are infected with Helicobacter pylori; however, no data exist about the association of vacA/cagA genotype/status with disease outcomes in them. We analyzed association of vacA/cagA genotypes/status of children's isolates with gastric inflammation status as the first step in H. pylori pathogenesis. Materials and methods: Antral biopsies for culture and histopathology were taken from 328 children in 1997-2009. vacA (s, m) alleles and cagA statuses of the isolates were determined by PCR. Histopathology was performed according to the Sydney system; gastritis was scored as normal, mild, moderate, severe, and follicular. Results: A total of 159 …