Medicine and Health Sciences Commons^™

Association Of Plasma Afatoxin With Persistent Detection Of Oncogenic Human Papillomaviruses In Cervical Samples From Kenyan Women Enrolled In A Longitudinal Study, Yan Tong, Philip Tonui, Elkanah Orang'o, Jianjun Zhang, Titus Maina, Kapten Muthoka, John Groopman, Joshua Smith, Erin Madeen, Aaron Ermel

Obstetrics and Gynaecology, East Africa

Background: Cervical cancer is caused by oncogenic human papillomaviruses (HR-HPV) and is common among Kenyan women. Identifcation of factors that increase HR-HPV persistence is critically important. Kenyan women exposed to afatoxin have an increased risk of HR-HPV detection in cervical specimens. This analysis was performed to examine associations between afatoxin and HR-HPV persistence.

Methods: Kenyan women were enrolled in a prospective study. The analytical cohort for this analysis included 67 HIVuninfected women (mean age 34 years) who completed at least two of three annual study visits and had an available blood sample. Plasma afatoxin was detected using ultra-high pressure liquid …

Urinary Aflatoxin M1 Concentration And Its Determinants In School-Age Children In Southern Ethiopia, Tafere Gebreegziabher Belay, Melanie Dean, Elilta Elias, Workneh Tsegaye, Barbara J. Stoecker

All Faculty Scholarship for the College of Education and Professional Studies

Aflatoxins are mycotoxins that can contaminate grains, legumes, and oil seeds. These toxic compounds are an especially serious problem in tropical and sub-tropical climates. The objective of this study was to raise awareness of aflatoxin exposure among primary school children in Shebedino woreda, southern Ethiopia, by measuring urinary aflatoxin M1 (AFM1). The study employed a cross-sectional design and systematic random sampling of children from eight schools in the district. The mean ± SD age of the children was 9.0 ± 1.8 years. Most (84.6%) households were food insecure with 17.9% severely food insecure. Urinary AFM1 was detected in more than …

Effect Of Chemical- And Toxin Binder-Treated Cotton Seed Cake On Milk Production, Milkcomposition, And Aflatoxin Concentration In Milk Of Nili Ravi Lactating Buffaloes, Murtaza Ali Tipu, Anjum Khalique, Muhammad Naveed Ul-Haque, Muhammad Qamer Shahid, Maqsood Akhtar, Mubashir Ilyas

Turkish Journal of Veterinary & Animal Sciences

Twelve multiparous Nili Ravi buffaloes were assigned to 1 of 3 treatments in a randomized, replicated 3 × 3 Latin square design to evaluate the effect of chemical (calcium propionate)- and toxin binder (sodium bentonite)-treated cotton seed cake (CSC) on milk yield and transfer of milk toxin (M1). Periods (20 days) were allocated for each treatment. In the 1st treatment (control), CSC with 100 μg/kg of aflatoxin B1 (AFB1) was fed to buffaloes. The 2nd and 3rd treatments were 0.5% calcium-propionate-treated CSC and 1.0% sodium-bentonite-treated CSC, respectively. Each group had 4 multiparous buffaloes. The buffaloes given 0.5% calcium propionate CSC …

Effects Of Aflatoxin B1 On The Submandibular Salivary Gland Of Albino Rats And Possible Therapeutic Potential Of Rosmarinus Officinalis: A Light And Electron Microscopic Study, Mohamed Shamel, Bassant Ashraf

Dentistry

Background: Aflatoxin B1 (AFB1), a highly toxic mycotoxin, is one of the contaminants of food items such as corn, rice, nuts, and flour. This study aimed to evaluate the effect of AFB1 on the histology and ultrastructure of the submandibular salivary glands (SMSG) of albino rats and examine the possible therapeutic effect of Rosmarinus officinalis extract. Methods: This study used 21 adult male albino rats equally divided into three groups as follows: Group C (saline-treated control group); Group A (AFB1 treated group) subjected to intraperitoneal injection of AFB1 (2 mg/kg) once daily for four weeks; Group R …

Effects Of Various Toxeat$^{®}$ Concentrations On Growth Performance, Immune Response, Cecal Microflora, And Gut Morphology In Broilers Fed With Aflatoxin Contaminated Diets, Soheila Sarrami, Parvaneh Jafari, Maryam Tajabadi Ebrahimi, Seyed Naser Mousavi, Seyed Abdollah Hosseini

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of Toxeat$^{®}$, a mycotoxin binder, on functional indices, immune response, intestinal microflora, and gut morphology in broiler chickens fed aflatoxin-contaminated diets. Three hundred Cobb 500 broilers were randomly divided into 6 treatments with 5 replicates, each with 10 birds. The treatments included T1: negative control (NC), T$_{2:}$ T1 + 1 g/ton aflatoxin (positive control: PC), T$_{3:}$ PC + 1 kg/ton Toxeat$^{®}$, T$_{4:}$ PC + 2 kg/ton Toxeat$^{®}$, T$_{5:}$ PC + 3 kg/ton Toxeat$^{®}$, T$_{6:}$ PC + 1 kg/ton Toxeat$^{®}$ without hydrated sodium calcium aluminosilicate. Growth performance, immune system response, and lactic acid …

Prevalence Of Aflatoxin-Associated Tp53r249s Mutation In Hepatocellular Carcinoma In Hispanics In South Texas, Jingjing Jiao, Weibo Niu, Ying Wang, Keith Baggerly, Yuanqing Ye, Xifeng Wu, Dewitt Davenport, Jose Luis Almeda, Monica Michelle Betancourt-Garcia, R. Armour Forse, Joseph B. Mccormick, Susan P. Fisher-Hoch

School of Medicine Publications and Presentations

We aimed to determine whether aflatoxin dietary exposure plays a role in the high incidence of hepatocellular carcinoma (HCC) observed among Hispanics in South Texas. We measured TP53R249S somatic mutation, hallmark of aflatoxin etiology in HCC, using droplet digital PCR and RFLP. TP53R249S mutation was detected in 3 of 41 HCC tumors from Hispanics in South Texas (7.3%). We also measured TP53R249S mutation in plasma cell-free DNA (cfDNA) from 218 HCC patients and 96 Hispanic subjects with advanced fibrosis or cirrhosis, from South Texas. The mutation was detected only in Hispanic and Asian HCC patients, and patients harboring TP53R249S mutation …

Impact Of Dietary Modification On Aflatoxicosis In Poultry, Xi Chen

Open Access Dissertations

Aflatoxin contamination in feed and feed ingredients is of high worldwide prevalence, posing serious risks to the livestock industry. A series of studies were conducted to better understand the comprehensive impact of aflatoxicosis and its interrelationships with dietary modifications in broiler chicks and ducks. Results of these studies emphasize the many factors that the severity of aflatoxicosis depends on, including animal species, age, presence of mycotoxin adsorbent, and concentration of major dietary nutrients. Exposure to 2 mg/kg cultured AFB1 led to reduced feed intake, BW gain, depressed feed efficiency, increased relative liver weight, negatively affected serum measures and complement activity, …

Aflatoxin Effect On Humoral And Mucosal Immune Responses Against Infectious Bronchitis Vaccine In Broilers, Babak Shahabi-Ghahfarokhi, Majid Gholami-Ahangaran, Mohsen Jafarian-Dehkordi

The Thai Journal of Veterinary Medicine

The aflatoxin effect on humoral and mucosal immunity against infectious bronchitis (IB) vaccine was studied in 225 one-day-old chicks. The chicks were divided in 3 equal groups. Treatment groups included group A: chickens fed a basal diet, and group B: chickens fed 3 ppm productive aflatoxin in a basal diet. The chickens in groups A and B were vaccinated against IB (at one and 10 days old) by H120 live attenuated vaccine. For group C, chickens did not receive aflatoxin and were not vaccinated against IB. All chickens received experimental diets from 3 to 28 days old, continuously. At 28 …

Calcium Montmorillonite Clay Reduces Urinary Biomarkers Of Fumonisin B₁ Exposure In Rats And Humans, Abraham Robinson, Natalie M. Johnson, A. Strey, John F. Taylor, Alicia Marroquin-Cardona, N. J. Mitchell, Evans Afriyie-Gyawu, Nii-Ayi Ankrah, Jonathan H. Williams, Jia-Sheng Wang, Pauline E. Jolly, R. J. Nachman, Timothy D. Phillips

Environmental Health Sciences Faculty Publications

Fumonisin B1 (FB1) is often a co-contaminant with aflatoxin (AF) in grains and may enhance AF’s carcinogenicity by acting as a cancer promoter. Calcium montmorillonite (i.e. NovaSil, NS) is a possible dietary intervention to help decrease chronic aflatoxin exposure where populations are at risk. Previous studies show that an oral dose of NS clay was able to reduce AF exposure in a Ghanaian population. In vitro analyses from our laboratory indicated that FB1 (like aflatoxin) could also be sorbed onto the surfaces of NS. Hence, our objectives were to evaluate the efficacy of NS clay to reduce urinary FB1 in …

Aflatoxin B1 Albumin Adducts In Plasma And Aflatoxin M1 In Urine Are Associated With Plasma Concentrations Of Vitamins A And E, Francis A. Obuseh, Pauline E. Jolly, Yi Jiang, Faisal M. B. Shuaib, John Waterbor, William O. Ellis, Chandrika J. Piyathilake, Renee A. Desmond, Evans Afriyie-Gyawu, Timothy D. Phillips

Evans Afriyie-Gyawu

Background: Although aflatoxin exposure has been shown to be associated with micronutrient deficiency in animals, there are few investigations on the effects of aflatoxin exposure on micronutrient metabolism in humans.
Objective: To examine the relationship between aflatoxin B1 (AFB1) albumin adducts (AF-ALB) in plasma and the aflatoxin M1 (AFM1) metabolite in urine and plasma concentrations of retinol (vitamin A) and α-tocopherol (vitamin E) in Ghanaians.
Methods: A cross-sectional study of 147 adult participants was conducted. Blood and urine samples were tested for aflatoxin and vitamins A and E levels.
Results: Multivariable analysis showed that participants with high AF-ALB (≥ 0.80 …

Association Between Birth Outcomes And Aflatoxin B1-Biomarker Levels Among Pregnant Women In Kumasi, Ghana, Faisal Mohammed Barbas Shuaib

All ETDs from UAB

Aflatoxins are fungal metabolites that contaminate staple food crops in many developing countries. Although studies have linked these toxins to adverse health outcomes, few studies have investigated the association between birth outcomes and aflatoxin B1-lysine adduct levels among pregnant women. The objective of this dissertation was to investigate the association between birth outcomes and aflatoxin B1- lysine adduct levels among pregnant women in Kumasi, Ghana. In a cross-sectional study of 755 pregnant women, Aflatoxin B1-lysine adduct (AF-ALB) levels were determined by High Performance Liquid Chromatography. AF-ALB associations were assessed as both continuous and categorical values. Analysis of variance (ANOVA) was …

Evaluation Of Aflatoxicosis In Hens Fed With Commercial Poultry Feed, Dharumadurai Dhanasekaran, Annamalai Panneerselvam, Nooruddin Thajuddin

Turkish Journal of Veterinary & Animal Sciences

The effect of aflatoxin in the growth of hens was histopathologically analyzed. Mycotoxigenic fungi were isolated and characterized as Aspergillus flavus and Aspergillus niger. The aflatoxin was extracted from Aspergillus flavus and their impact on the growth pattern of hens was evaluated. The histopathological analysis reveals that more lesions were found in the vital organs of hens in comparison with the control chick group. In the present study, it is concluded that the quality of poultry feed plays the most important role in a poultry farm. It is suggested that the use of chicks resistant to aflatoxicosis will help in …

Effects Of Two Different Types Of Montmorillonite On Growth Performance And Serum Profiles Of Broiler Chicks During Aflatoxicosis, Yinghua Shi, Zirong Xu, Yu Sun, Chengzhang Wang, Jianlei Feng

Turkish Journal of Veterinary & Animal Sciences

Experiments were conducted to compare the efficacy of montmorillonite (M) and montmorillonite nanocomposite (MN) to reduce the toxicity of aflatoxin (AF) in broiler chicks from 0 to 42 days of age. A total of 240 chicks were assigned to 6 dietary treatment groups (0 g of adsorbents and 0 mg of AF/kg feed, 3 g of M/kg feed, 3 g of MN/kg feed, 0.11 mg of AF/kg feed, 0.11 mg of AF plus 3 g of M/kg feed, and 0.11 mg of AF plus 3 g of MN/kg feed). Compared to the control, AF alone significantly decreased ADG and feed …

Effects Of Dietary Aflatoxin And Hydrate Sodium Calcium Aluminosilicate On Triiodothyronine, Thyroxine, Thyrotrophin And Testosterone Levels In Quails, Gökhan Eraslan, Mehmet Akdoğan, Bi̇lal Cem Li̇man, Murat Kanbur, Namik Deli̇baş

Turkish Journal of Veterinary & Animal Sciences

This study was performed on 80 male 14-day-old Coturnix coturnix japonica breed quails. The quails were divided into 8 groups with 10 animals in each as 1 control and 7 trial groups. While the control group was fed a commercial basal ration, groups 2-8 received, respectively, 2.5 g/kg feed hydrate sodium calcium aluminosilicate (HSCAS), 5.0 g/kg feed HSCAS, 10.0 g/kg feed HSCAS, 2.5 ppm aflatoxin (AF B_1 78.30%, AF B_2 14.60%, AF G_1 4.50%, AF G_2 2.60%); 2.5 ppm AF with 2.5 g/kg feed HSCAS, 2.5 ppm AF with 5.0 g/kg feed HSCAS, and 2.5 ppm AF with 10.0 g/kg …

Aflatoxin Contamination Of Commercial Maize Products During An Outbreak Of Acute Aflatoxicosis In Eastern And Central Kenya, Karen E. Gieseker, Lauren Lewis, Mary Onsongo, Henry Njapau, Helen Schurz Rogers, George Luber, Stephanie Kieszak, Jack Nyamongo, Lorraine Backer, Abdikher Mohamud Dahiye, Ambrose Misore, Kevin Decock, Carol Rubin, Kenya Aflatoxicosis Investigation Group

Public Health Faculty Publications

n April 2004, one of the largest aflatoxicosis outbreaks occurred in rural Kenya, resulting in 317 cases and 125 deaths. Aflatoxin-contaminated homegrown maize was the source of the outbreak, but the extent of regional contamination and status of maize in commercial markets (market maize) were unknown. We conducted a cross-sectional survey to assess the extent of market maize contamination and evaluate the relationship between market maize aflatoxin and the aflatoxicosis outbreak. We surveyed 65 markets and 243 maize vendors and collected 350 maize products in the most affected districts. Fifty-five percent of maize products had aflatoxin levels greater than the …

Case-Control Study Of An Acute Aflatoxicosis Outbreak, Kenya, 2004, Karen E. Gieseker, Eduardo Azziz-Baumgartner, Kimberly Lindblade, Helen Schurz Rogers, Stephanie Kieszak, Henry Njapau, Rosemary Schleicher, Leslie F. Mccoy, Ambrose Misore, Kevin Decock, Carol Rubin, Lawrence Slutsker, Aflatoxin Investigative Group

Public Health Faculty Publications

OBJECTIVES: During January-June 2004, an aflatoxicosis outbreak in eastern Kenya resulted in 317 cases and 125 deaths. We conducted a case-control study to identify risk factors for contamination of implicated maize and, for the first time, quantitated biomarkers associated with acute aflatoxicosis.

DESIGN: We administered questionnaires regarding maize storage and consumption and obtained maize and blood samples from participants.

PARTICIPANTS: We recruited 40 case-patients with aflatoxicosis and 80 randomly selected controls to participate in this study.

EVALUATIONS/MEASUREMENTS: We analyzed maize for total aflatoxins and serum for aflatoxin B1-lysine albumin adducts and hepatitis B surface antigen. We used regression and survival …

The Effects Of Aflatoxin And Sodium Bentonite Combined And Alone On Some Blood Electrolyte Levels In Broiler Chickens, Gökhan Eraslan, Di̇nç Eşsi̇z, Mehmet Akdoğan, Fatma Şahi̇ndokuyucu, Levent Altintaş

Turkish Journal of Veterinary & Animal Sciences

The aim of the study is to determine the effects of aflatoxin and sodium bentonite given alone and in combination on some of blood electrolyte levels in broiler chicks. For this aim, 72 male one-day-old Avian broiler chicks were used. The animals were separated into 6 groups, as one control (Group 1) and the others trial (Groups 2 to 6). While commercial feed was given to Group 1 (control), the other groups received feed containing 0.25% sodium bentonite, 0.50% sodium bentonite, 1 ppm aflatoxin (approximately, 85% B_1, 10% B_2, 3% G_1 and 2% G_2), 1 ppm aflatoxin with 0.25% sodium …

The Effects Of Aflatoxins On Oxidative Stress In Broiler Chickens, Gökhan Eraslan, Mehmet Akdoğan, Ender Yarsan, Fatma Şahi̇ndokuyucu, Di̇nç Eşsi̇z, Levent Altintaş

Turkish Journal of Veterinary & Animal Sciences

Sixty 1-day-old male Ross-PM3 broiler chickens were used in this study. The chickens were divided into 5 groups, including one control and 4 experimental. The control group was fed commercial broiler feed free from aflatoxin (AF), whereas the experimental groups, namely, groups 2, 3, 4 and 5 were given feed containing, respectively, 0.05 ppm, 0.1 ppm, 0.5 ppm and 1.0 ppm of AF (approximately, 81.30% AF B_{1}, 10.40% AF B_{2}, 5.75% AF G_{1} and 2.55% AF G_{2}) for 45 days. Blood samples were collected on days 15, 30 and 45 of the study. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase …

Efficacy Of Tribasic Copper Chloride (Tbcc) To Reduce The Harmful Effects Of Aflatoxin In Broilers, Sefa Çeli̇k, Zeynep Erdoğan, Suat Erdoğan, Ramazan Bal

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to evaluate the effects of copper as tribasic copper chloride on serum biochemical values and growth performance of broilers intoxicated with aflatoxins. Aflatoxins significantly decreased the level of albumin (P < 0.001), total protein (P < 0.001) and total cholesterol (P < 0.01), and increased the activities of alanine amino transferase, L-lactic dehydrogenase and alkaline phosphatase (P £ 0.001) in serum. These AF-induced changes were significantly improved by adding tribasic copper chloride to the diet. The decrease in serum copper concentration induced by aflatoxin was reversed by tribasic copper chloride. Serum zinc concentrations were not affected in tribasic copper chloride and tribasic copper chloride plus aflatoxin groups. Serum iron levels decreased in all the treatment groups significantly (P < 0.05). Aflatoxins caused significant decrease in the body weight gain and significant increase in the feed conversion ratio. Adverse effects of aflatoxins on feed conversion ratio were reversed by tribasic copper chloride supplementation to the diet. It is suggested that tribasic copper chloride might be used for reducing the adverse effects of aflatoxins in broiler production.

The Relationship Between Lameness, Fertility And Aflatoxin In A Dairy Cattle Herd, Serhat Özsoy, Kemal Altunatmaz, Huri̇ye Horoz Kaya, Güven Kaşikçi, Serhat Alkan, Tanay Bi̇lal

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the relationship between aflatoxins taken with feed, laminitis, lameness and impaired fertility. Lesions were identified in the claw and hock region, causing lameness in 45 cattle in an establishment of 300 Holstein dairy cattle. Of these lame cattle, 27 had cystic ovaries and 10 had cystic ovaries together with clinical metritis. The increase in lameness and fertility problems occurring in this herd, living under the same management and feeding conditions, was determined to start with the change in feed concentrates and, in order to investigate the relationship with mycotoxins, Aflatoxin B_1 (AFB_{1}) analysis …

Effect On Aflatoxin Formation Of Different Storage Conditions In Tulum Cheese Inoculated With Aspergillus Parasiticus Nrrl 2999, Mustafa Gürses, Ahmet Erdoğan, Selahatti̇n Sert

Turkish Journal of Veterinary & Animal Sciences

Tulum cheese was divided into 2 equal parts. One part was inoculated with 10^4 spores/g of aflatoxin-producing Aspergillus parasiticus NRRL 2999 strain. The other part, the control group, was not inoculated. All the samples were ripened in glass jars at 2 different temperatures (10 ± 1 and 20 ± 1 °C) and relative humidities (85 ± 2% and 95 ± 2%) and analyzed for aflatoxin formation on days 1, 15, 30, 45, 60 and 90 of storage using thin layer chromatography. Aflatoxins were determined in the inoculated samples after 15 days with incubation at 20 ± 1 °C, while they …

The Effect Of Aflatoxin On Kidney Function In Broiler Chicks, Gökhan Eraslan, Erdal Karaöz, Ali̇ Bi̇lgi̇li̇, Mehmet Akdoğan, Meral Öncü, Di̇nç Eşsi̇z

Turkish Journal of Veterinary & Animal Sciences

In this study, eighty, day-old female Avion breed, broiler chicks were used. Chicks were separated into five groups, including one control. While the control group (Group I) received normal feed, the experimental groups received feed supplemented with aflatoxin at the rate of 0.05 ppm (Group II), 0.1 ppm (Group III), 0.5 ppm (Group IV), and 1 ppm (Group V) for 45 days. On days 15, 30 and 45 blood was taken from the control and experimental animals in order to perform biochemical analyses and kidney tissue was dissected for histopathological examination. As a result, a number of pathological findings were …

The Contamination Of Aflatoxin In Food Products, W. Waenlor, V. Wiwanitkit

Chulalongkorn Medical Journal

No abstract provided.

The Effect Of Aflatoxin B1 On The Vitamin A Storage In The Livers Of Broiler Chicks, Berri̇n Salmanoğlu

Turkish Journal of Veterinary & Animal Sciences

The effect of AFB1 in feed on the liver and serum vitamin A and b-carotene levels and the ability of the liver to stor vitamin A in broiler chicks were investigated. Twenty day-old Ross broiler chicks were randomly allocated into 4 groups with 5 each in the control group, group I, group II, and group III. The chicks of the control group and group I were fed ad libitum on broiler chick growing feed. The chicks of group II and group III were fed ad libitum on feed with AFB1 (4 ppm/kg) feed. On the seventh day, vitamin A palmitate …

Aflatoxin B1 Metabolism In Mammalian Pulmonary Tissue, J. Michael Eichelberger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Aflatoxin B₁ (AFB₁) is a potent dietary hepatocarcinogen and may be a lung carcinogen when inhaled. To study the relative ability of lung and liver to metabolize AFB₁, a susceptible (Swiss-Webster rat) and resistant species (Syrian golden hamster) were pretreated with inducing agents in order to identify specific AFB₁ metabolizing enzymes in each tissue.

Analysis of AFB₁-exo-epoxide (AFBO) formation, O-dealkynation assays, and protein immunoblots demonstrated that cytochrome P450 (CYP) 1A proteins were overexpressed in both the lung and liver of hamsters pretreated with 3-methylchyolanthrene (3-MC). Only CYP1A1 was expressed in the lung …