Medicine and Health Sciences Commons^™

Distal Urogenital Anatomy And Epithelial Morphology In The Male Cajun Chorus Frog, Pseudacris Fouquettei (Anura: Hylidae), S.E. Trauth

Journal of the Arkansas Academy of Science

I examined the microanatomy of the distal urogenital system in male Cajun Chorus Frogs (Pseudacris fouquettei) from a small sample (n = 6) collected in central Arkansas in March 2018 and February 2023. Specifically, my primary objectives were to investigate the following: (1) the paired Wolffian (urogenital) ducts caudally from the kidneys to their merging with the urodeum of the cloaca, (2) the structure of seminal vesicles (if present), and (3) the epithelial morphologies among the distal urogenital tract, the posterior alimentary canal, and cloaca. My histological analysis confirmed the presence of ampullae (sperm storage structures) as mid-duct expansions of …

Use Of Google Earth Streetview To Evaluate Nesting By The Cliff Swallow (Petrochelidon Pyrrhonota) In Lowlands Of Eastern Arkansas, With Reports Of New Records, R. Tumlison, Z. Autrey

Journal of the Arkansas Academy of Science

Cliff Swallows (Petrochelidon pyrrhonota) typically construct their mud nests under the overhangs of cliffs. However, construction of concrete bridges and overpasses has provided suitable nesting structure in the last few decades. As the number of such nesting sites have increased, the breeding range also has expanded. We evaluated the use of Google Earth Streetview as a means of cheaply harvesting data regarding nesting distribution. The gourd-shaped nests of Cliff Swallows were easily discernible via available imagery, and we were able to document previously unreported nest sites of this species in 6 counties in eastern Arkansas (5 from imagery, 1 from …

Shell Damage Patterns In Limpkin Mussel Middens At Bois D’Arc Lake, Arkansas, G.R. Graves

Journal of the Arkansas Academy of Science

The Limpkin (Aramus guarauna) is rapidly colonizing the lower Mississippi Valley from recently established Gulf Coast populations. Anecdotal reports indicate that it feeds on freshwater mussels and gastropods in Arkansas, but without documentation. Here I report an analysis of Limpkin shell middens from Bois d’Arc Lake in Hempstead Co. Limpkins at this location preyed heavily on Pyganodon grandis (Giant Floater), a large mussel species widely distributed in the Mississippi Valley. Limpkins employ vigorous bill blows to pierce holes in mussels and usually attack the anterior end of the shell. Video and photographs of feeding birds provide new insight on the …

Rapid Expansion Of The Limpkin Aramus Guarauna Across Anglo-America, A. Alam, R. Kaddoura, J. Jackson, R. Kannan

Journal of the Arkansas Academy of Science

We quantitatively document and describe the recent rapid expansion of the Limpkin Aramus guarauna across Anglo-America (United States and Canada). Using data downloaded from eBird (www.eBird.org), we present graphs showing the explosive growth in reports of number of individual birds and frequencies in checklists for this species in several parts of Anglo- America outside Florida (its traditional Anglo- American range). We also provide geographic progression maps to illustrate the expansion through time (2013-2023) and furnish qualitative information on foods consumed by the bird in new areas, based on a survey of 274 images uploaded by citizen scientists in eBird. Possible …

X-Ray Diffraction And Petrographic Analysis Of Magnet Cove Carbonatite Core, Arkansas, R.E. Mero, D. Mayo, M.P. Testa

Journal of the Arkansas Academy of Science

Geothermal activity such as hot springs are known to precipitate calcium carbonate (CaCO3) minerals, producing rocks such as tufa, travertine and sometimes associated with carbonatite. The precipitation of CaCO is caused by the reduction of CO, which is less soluble in warmer waters. Geothermal heating of water in natural springs drives this precipitation of CaCO3 in areas of Arkansas including Magnet Cove. Magnet Cove, Arkansas is an alkalic igneous rock complex that is composed of a series of ring dikes post-Mississippian in age. These dikes have intruded into faulted and folded Paleozoic sedimentary rocks. One of these igneous dikes is …

The Management And Outcomes Of Hepatocellular Carcinoma In Sub-Saharan Africa: A Systematic Review., Sanju Sobnach, Urda Kotze, Wendy Spearman, Mark Sonderup, Pueya Nashidengo, Chikwendu Ede, Elie Keli, Onesai Chihaka, Luiz Zerbini, Yifan Li, Karan Gandhi

General Surgery, East Africa

Background: Hepatocellular carcinoma (HCC) is a leading cause of mortality in sub-Saharan Africa (SSA). This systematic review aimed to appraise all population-based studies describing the management and outcomes of HCC in SSA.

Methods: A systematic review based on a search in PubMed, PubMed Central, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), AfricaWide and Cochrane up to June 2023 was performed. PRISMA guidelines for systematic reviews were followed. The study protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO) (registration no: CRD42022363955).

Results: Thirty-nine publications from 15 of 48 SSA countries were …

Carotenoids And The Associations With Gut Microbiota Bacterial Populations In Chronic Kidney Disease Hemodialysis Patients, Andrea Kendall

Theses and Dissertations

Background: With increased prevalence and mortality of chronic kidney disease (CKD), treatment by renal replacement therapy using hemodialysis (HD) has increased in parallel. Emerging evidence suggests that gut microbiota play an important role in the progression of CKD; influential dietary factors have not been studied exhaustively to date.

Objective: To explore the relationships between dietary carotenoid intake, the abundance of specific gut microbiota, and symptom-related outcomes in patients with CKD on HD.

Methods: This cross-sectional study investigated the gut microbiota characteristics relative to carotenoid intake in CKD subjects on HD in central Illinois (n = 20). The Block Dialysis …

Communication Supports To Enhance The Community Participation Of Young Children With Complex Communication Needs: A Utilization-Focused Evaluation, Amy L. Yacucci

Theses and Dissertations

This utilization-focused evaluation study aimed to describe augmentative and alternative communication (AAC) supports to enhance communication accessibility for children with complex communication needs in a children’s museum. Utilizing feedback from museum staff and parents of children with communication disabilities, the study sought to identify practical strategies to improve the effectiveness of AAC supports in creating more inclusive community spaces. A utilization-focused evaluation framework (Patton & Campbell-Patton, 2022) guided the assessment of embedded-aided communication supports within the museum, ensuring that findings were relevant and actionable for stakeholders. The evaluation revealed three key insights: (a) the importance of communication access through multiple …

Improving Patient Engagement Post-Percutaneous Coronary Intervention Using The Systematic Introduction Of A Cardiac Rehabilitation Bundle In A Community Hospital: A Quality Improvement Project, Rebecca Vela

Doctor of Nursing Practice Final Project Abstract

PURPOSE This quality improvement project aimed to increase patient participation and program completion rates in cardiac rehabilitation (CR) following percutaneous coronary intervention (PCI) by 10% of the historical baseline within 3 months.

BACKGROUND Heart disease is the leading cause of death in the United States. PCI is a standard treatment for symptomatic heart disease. CR is a post-PCI secondary strategy proven to reduce mortality and improve quality of life. The project addressed below-average CR attendance at a community hospital in the greater Houston area.

METHODOLOGY An evidence-based, multidisciplinary bundle that included education, signage, defined quality metrics and dashboard, patient-centered care, …

Decreasing Compassion Fatigue In Inpatient Nurses Using Debriefing And Group Care Program, Hanh Nguyen

Doctor of Nursing Practice Final Project Abstract

Purpose: The quality improvement project's purpose was to use a group care program to decrease compassion fatigue (work-related stress) in nurses. The project aimed to reduce compassion fatigue in nurses by 25% and nurse turnover rates by 10% post-intervention.

Background: The project was implemented on two inpatient units in Texas. Decrease in compassion fatigue can help improve nurse well-being and nurse retention, promoting safe quality nursing care.

Methodology: The Professional Quality of Life Scale (ProQOL) survey measured compassion satisfaction and burnout pre-intervention. Twelve weekly program sessions were completed using strategies: debriefing, breathing exercise, and muscle relaxation. A …

Implementation Of Same Day Cervical Cancer And Chlamydia Screening In A University Clinic Setting, Amanda Rodriguez

Doctor of Nursing Practice Final Project Abstract

Implementation of same day cervical cancer and chlamydia screening in a university clinic setting

PURPOSE The purpose of this quality improvement project was to implement a screening questionnaire in a university student health center to increase the rates of up-to-date cervical cancer and chlamydia screening.

BACKGROUND The project was implemented at a university-based student health center with a student population of 31,559 with 60.3% of the population being female.

METHODOLOGY A screening questionnaire regarding pap smear results and last chlamydia testing was developed for all female students age 18-65 to complete at check in for their appointments. Medical staff were …

Impact Of A Nurse Manager Development Program On Manager Competency And Intent To Leave, Yashamika Short

Doctor of Nursing Practice Final Project Abstract

PURPOSE The purpose of this Quality Improvement Project is to create an evidence-based onboarding and orientation process for novice and assistant nurse managers that positively impacts nurse manager competency and intent to leave.

BACKGROUND Many organizations don’t provide formal leadership training for their nurse managers. Nurse managers are typically selected for their positions based on clinical expertise rather than leadership and management competency.

METHODOLOGY American Organization for Nursing Leadership Manager Competency Assessment tool and Dr. Nora Warshawsky Nurse Manager Intent to Leave Survey were utilized pre/post training. The framework has 3 domains: the science (managing the business), the art (leading …

Enhancing Medication Safety Through Standardized Protocols And Skills Competency Checklist: A Quality Improvement Project In Group Homes For Individuals With Intellectual Disabilities, Mary Chilaka

Doctor of Nursing Practice Final Project Abstract

PURPOSE

This quality improvement (QI) project aimed to enhance medication safety in group homes for individuals with intellectual disabilities by implementing standardized protocols and a skills competency checklist for Unlicensed Assistive Personnel (UAPs).

BACKGROUND

Medication errors in group homes often result from inconsistent training and oversight among UAPs. This project addressed these gaps by introducing structured training, a competency checklist, and workflow improvements to enhance adherence to best practices.

METHODOLOGY

Using the Plan-Do-Study-Act (PDSA) cycle, key interventions included bi-weekly training, hands-on shadowing, competency assessments, and "do not disturb" signage to minimize distractions. Data collection included pre- and post-training surveys, weekly …

Relationships Of Secondary Traumatic Stress, Compassion Fatigue, And Compassion Satisfaction Among Maternity Nurses Caring For Patients Who Succumbed To Maternal Mortality, Debra Joy Mendelson

Theses & Dissertations

Background:

Maternity nurses work where positive outcomes are the norm, leaving them more vulnerable to the adverse effects of dealing with death. Limited data indicate that caring for a maternity patient who dies may result in nurses developing secondary traumatic stress and associated compassion fatigue, which may affect their job performance. Research is warranted to promote a better understanding of nurses’ experiences, especially since the increase in maternal mortality has resulted in more nurses being exposed to maternal death.

Purpose:

The purpose of this study was to explore relationships between secondary traumatic stress, compassion fatigue, and compassion satisfaction of maternity …

Autoimmunities After Covid: An Interview With Cindy Patton, Cindy Patton, Travis Alexander, Nishant Shahani

English Faculty Publications

Taken collectively, Patton’s scholarship and activism has laid the foundation for insights in the health humanities, particularly AIDS studies, that consider the inextricable connections between epidemiology and ideology. Patton’s theorizations of stigma and discrimination patterns, her deconstruction of “truth” discourses subtending science, her critical re-evaluations of axioms associated with risk, safe sex, community, and knowledge production have been crucial interventions in the understanding of health and illness as cultural and discursive scripts. Among Patton’s most enduring contributions has been her theorization of how “African AIDS” was invented and circulated—that is, the notion of geographically bifurcated HIV pandemics split by the …

Mindfulness Meditation To Address Stress And Quality Of Work Life, Mari Eastland

Doctor of Nursing Practice Final Project Abstract

PURPOSE

The purpose of this quality improvement project was to implement the mindfulness intervention of the 3-minute breathing space (3MBS). The project was intended to decrease stress by 5% and increase professional quality of work life by 5% in healthcare workers.

BACKGROUND

The project was implemented in an outpatient center for rehabilitation and sports medicine in a rural community 60 miles outside of Houston, Texas.

METHODOLOGY

The Perceived Stress Scale and the Professional Quality of Life scale were used in a pre- and post-intervention format to track results. Daily usage of the 3MBS was tracked by participants on a physical …

N(Alpha)-Acetyltransferase 40-Mediated Histone Acetylation Plays An Important Role In Ecdysone Regulation Of Metamorphosis In The Red Flour Beetle, Tribolium Castaneum, Sharath Chandra Gaddelapati, Smitha George, Anilkumar Moola, Karthi Sengodan, Subba Reddy Palli

Entomology Faculty Publications

Histone acetylation, a crucial epigenetic modification, is governed by histone acetyltransferases (HATs), that regulate many biological processes. Functions of HATs in insects are not well understood. We identified 27 HATs and determined their functions using RNA interference (RNAi) in the model insect, Tribolium castaneum. Among HATs studied, N-alpha-acetyltransferase 40 (NAA40) knockdown caused a severe phenotype of arrested larval development. The steroid hormone, ecdysone induced NAA40 expression through its receptor, EcR (ecdysone receptor). Interestingly, ecdysone-induced NAA40 regulates EcR expression. NAA40 acetylates histone H4 protein, associated with the promoters of ecdysone response genes: EcR, E74, E75, and HR3, and causes an increase …

A Social Media Analysis Of Patient-Perceived Complications Following Periacetabular Osteotomy (Pao): A Retrospective Observational Study, Bretton Laboret, Ryan Bialaszewski, John M. Gaddis, Emily Middleton, Brittany Kendall, Katie Lynch, Adina Stewart, Joel Wells

School of Medicine Publications

Background: Social media is a popular resource for patients seeking medical information and sharing experiences. Periacetabular osteotomy (PAO) is an accepted treatment for symptomatic acetabular dysplasia with a low published complication profile in specialty centers. Little is known regarding patient reporting of complications on social media following PAO. The purpose of this study was to describe the patient-perceived complications of PAO posted on social media and analyze how additional factors (postoperative timeframe, concomitant surgery) correlate with these complication posts.

Methods: Facebook and Instagram were queried from 02/01/18-02/01/23; Twitter was searched over an extended range back to 02/01/11. Facebook posts (1054) …

Hour Of Life At Enteral Feeding Initiation And Associated Clinical Morbidity In Extremely Low-Birth-Weight Infants, Melissa Thoene, Lauren Ridgway, Elizabeth Lyden, Ann Anderson-Berry

Journal Articles: Pediatrics

BACKGROUND/OBJECTIVES: Identifying nutritional interventions in extremely low-birth-weight (ELBW) infants (<1000 >g) that are associated with favorable clinical outcomes is important. Delayed enteral feeding initiation (>3 days) has been associated with increased odds of developing morbidity. Therefore, the aim of this study is to evaluate the relationship between hour of life at enteral feeding initiation and associated clinical outcomes.

METHODS: An IRB-approved retrospective chart review evaluated ELBW infants. Birth acuity was evaluated using CRIB II scoring and incidence of various morbidities (bronchopulmonary dysplasia (BPD), retinopathy of prematurity (ROP), necrotizing enterocolitis (NEC), and spontaneous intestinal perforation (SIP)) and mortality was assessed …

Human Cell Modeling Of Cdkl5-Deficiency Disorder With Patient-Derived Organoids And Hd-Mea, Alyssa Paynton

Cal Poly Humboldt theses and projects

CDKL5 deficiency disorder (CDD) is an X-linked disorder that leads to severe cognitive impairments as well as infantile epilepsy. The only FDA approved therapy helps to reduce seizure frequency, however there are currently no cures approved. While murine models have proven useful in characterizing CDD, there are limitations due to differences in brain structure and development across species that may lead to the reported lack of spontaneous seizures in the mouse, a hallmark in humans affected by this disorder. By the utilization of patient-specific iPSCs generated into cerebral organoids, a more comprehensive and in-depth analysis into rare neurological disorders, such …

Changes In Deadlift Six Repetition Maximum, Countermovement Jump Performance, Barbell Velocity, And Perceived Exertion Over The Duration Of A Microcycle, Stuart N. Guppy, Tsuyoshi Nagatani, Wayne C. K. Poon, Kristina L. Kendall, Jason P. Lake, G. Gregory Haff

Research outputs 2022 to 2026

The primary aim of this study was to investigate the stability of the six-repetition maximum (6RM) deadlift over the length of a five-day microcycle and whether the fatigue induced by maximal effort testing detrimentally impacted preparedness. Twelve participants performed four testing sessions, comprising a one-repetition maximum test and three 6RM tests separated by 48 hours. Countermovement jumps were performed before each testing session, and barbell velocity was measured during each warm-up set to assess changes in preparedness. The 6RM deadlift was not statistically different between any of the testing sessions (p  =  .056; η_p²  =  0.251). Similarly, …

Excitatory Drive To Spinal Motoneurones Is Necessary For Serotonin To Modulate Motoneurone Excitability Via 5-Ht2 Receptors In Humans, Tyler T. Henderson, Janet L. Taylor, Jacob R. Thorstensen, Justin J. Kavanagh

Research outputs 2022 to 2026

Serotonin modulates corticospinal excitability, motoneurone firing rates and contractile strength via 5-HT2 receptors. However, the effects of these receptors on cortical and motoneurone excitability during voluntary contractions have not been explored in humans. Therefore, the purpose of this study was to investigate how 5-HT2 antagonism affects corticospinal and motoneuronal excitability with and without descending drive to motoneurones. Twelve individuals (aged 24 ± 4 years) participated in a double-blind, placebo-controlled, crossover study, whereby the 5-HT2 antagonist cyproheptadine was administered. Transcranial magnetic stimulation (TMS) was delivered to the motor cortex to produce motor evoked potentials (MEPs), and electrical stimulation at the cervicomedullary …

In Vivo Human Medial Gastrocnemius Fascicle Behaviour And Belly Gear During Submaximal Eccentric Contractions Are Not Affected By Concentric Fatiguing Exercise, Matheus Daros Pinto, Kazunori Nosaka, Anthony J. Blazevich

Research outputs 2022 to 2026

Changes in muscle geometry and belly gearing during eccentric contractions influence fibre strain and susceptibility to muscle damage. They are modulated by the interaction between connective tissues and intracellular-intrafascicular fluid pressures and external pressures from neighbouring structures. Fatiguing exercise triggers fluid shifts (muscle swelling) and muscle activation changes that may influence these modulators. Our purpose was to measure medial gastrocnemius (MG) geometric changes in vivo during eccentric contractions before and after maximal concentric muscle work to test the hypothesis that fatigue would reduce fascicle rotation and muscle gear and provoke greater fascicle strain. Submaximal eccentric plantar flexor contractions at 40% …

Quantitative Comparison Of Presenilin Protein Expression Reveals Greater Activity Of Ps2-Γ-Secretase, Melissa K. Eccles, Nathan Main, Rodrigo Carlessi, Ayeisha M. Armstrong, Miheer Sabale, Brigid Roberts-Mok, Janina E. E. Tirnitz-Parker, Mark Agostino, David Groth, Paul E. Fraser, Giuseppe Verdile

Research outputs 2022 to 2026

γ-secretase processing of amyloid precursor protein (APP) has long been of interest in the pathological progression of Alzheimer's disease (AD) due to its role in the generation of amyloid- . The catalytic component of the enzyme is the presenilins of which there are two homologues, Presenilin-1 (PS1) and Presenilin-2 (PS2). The field has focussed on the PS1 form of this enzyme, as it is typically considered the more active at APP processing. However, much of this work has been completed without appropriate consideration of the specific levels of protein expression of PS1 and PS2. We propose that expression is an …

The Gut Microbiome Modulates Associations Between Adherence To A Mediterranean-Style Diet, Abdominal Adiposity, And C-Reactive Protein In Population-Level Analysis, Amy Jennings, Tilman Kühn, Nicola P. Bondonno, Sabina Waniek, Corinna Bang, Andre Franke, Jan Kassubek, Hans-Peter Müller, Marcus Both, Katharina S. Weber, Wolfgang Lieb, Aedín Cassidy

Research outputs 2022 to 2026

Background: Adherence to a Mediterranean-style dietary pattern is likely to have variable effects on body composition, but the impact of gut microbiome on this relationship is unknown. Objectives: To examine the potential mediating effect of the gut microbiome on the associations between Alternate Mediterranean Diet (aMed) scores, abdominal adiposity, and inflammation in population-level analysis. Design: In a community-based sample aged 25 to 83 y (n = 620; 41% female) from Northern Germany, we assessed the role of the gut microbiome, sequenced from 16S rRNA genes, on the associations between aMed scores, estimated using validated food-frequency questionnaires, magnetic resonance imaging-determined visceral …

Topical Antibiotics Prophylaxis For Infections Of Indwelling Pleural/Peritoneal Catheters (Tap-Ipc): A Pilot Study, Estee P. M. Lau, Sam Faber, Chloe Charlesworth, Sue Morey, Sona Vekaria, Pierre Filion, Aron Chakera, Y. C.Gary Lee

Research outputs 2022 to 2026

Background and Objective: Indwelling pleural catheter (IPC) and indwelling peritoneal catheter (IPeC) have established roles in the management of malignant pleural and peritoneal effusions but catheter-related infections remain a major concern. Topical mupirocin prophylaxis has been shown to reduce peritoneal dialysis catheter infections. This study aimed to assess the (i) compatibility of IPC with mupirocin and (ii) feasibility, tolerability and compliance of topical mupirocin prophylaxis in patients with an IPC or IPeC. Methods: (i) Three preparations of mupirocin were applied onto segments of IPC thrice weekly and examined with scanning electron microscope (SEM) at different time intervals. (ii) Consecutive patients …

Editorial: Neurotoxins In Alzheimer's Disease And Other Dementias, Alexandre Henriques, Philippe L. L. Poindron, Binosha Fernando, Kevin N. Hascup

Research outputs 2022 to 2026

Alzheimer's disease (AD) and other dementias are neurodegenerative disorders characterized by a progressive decline in cognition and independence from activities of daily living. Dementia is multifactorial with numerous risk factors including age, genes, molecules, lifestyle, and environmental contributions to disease onset and progression. In recent years, an emerging focus on neurotoxins has added a new layer of complexity to our understanding of dementia. This editorial aims to discuss recent updates regarding the role of neurotoxins in the pathogenesis of dementia.

Investigation Of Campylobacter Concisus Gastric Epithelial Pathogenicity Using Ags Cells, Christopher Y. M. Luk, Seul A. Lee, Nicholas Naidovski, Fang Liu, Alfred C. Y. Tay, Liang Wang, Stephen Riordan, Li Zhang

Research outputs 2022 to 2026

Campylobacter concisus is an oral bacterium. Recent studies suggest that C. concisus may be involved in human gastric diseases. The mechanisms, however, by which C. concisus causes human gastric diseases have not been investigated. Here we examined the gastric epithelial pathogenicity of C. concisus using a cell culture model. Six C. concisus strains and the human gastric epithelial cell line AGS cells were used. IL-8 produced by AGS cells after incubation with C. concisus was measured using enzyme-linked immunosorbent assay (ELISA), and AGS cell apoptosis was determined by caspase 3/7 activities. The effects of C. concisus on actin arrangement in …

Staff Perceptions On The Effectiveness Of Grip-S, A New Approach To Clinical Supervision Incorporating Safewards: An Interpretive Phenomenological Analysis, Jennifer Hamilton, Amanda Cole, Richard Bostwick, Irene Ngune

Research outputs 2022 to 2026

This study explored the impact of an innovative approach to clinical supervision for mental health nurses which integrates Safewards, named Group Reflective integrated Practice with Safewards–GRiP-S. Qualitative data was collected through 10 individual semi-structured interviews with nursing staff who had participated within the clinical supervision approach. Interviews provided insights into the nursing staff’s perception and experience of the clinical supervision approach. Through interpretive phenomenological analysis six themes emerged (i) illuminating embodied practice of Safewards, (ii) building confidence through empowering connections, (iii) creating a culture of positive change, (iv) identifying internal motivation for and external barriers to supervision engagement, (v) navigating …

Airway Epithelium Respiratory Illnesses And Allergy (Aerial) Birth Cohort: Study Protocol, Elizabeth Kicic-Starcevich, David G. Hancock, Thomas Iosifidis, Patricia Agudelo-Romero, Jose A. Caparros-Martin, Yuliya V. Karpievitch, Desiree Silva, Lidija Turkovic, Peter N. Le Souef, Anthony Bosco, David J. Martino, Anthony Kicic, Susan L. Prescott, Stephen M. Stick

Research outputs 2022 to 2026

Introduction: Recurrent wheezing disorders including asthma are complex and heterogeneous diseases that affect up to 30% of all children, contributing to a major burden on children, their families, and global healthcare systems. It is now recognized that a dysfunctional airway epithelium plays a central role in the pathogenesis of recurrent wheeze, although the underlying mechanisms are still not fully understood. This prospective birth cohort aims to bridge this knowledge gap by investigating the influence of intrinsic epithelial dysfunction on the risk for developing respiratory disorders and the modulation of this risk by maternal morbidities, in utero exposures, and respiratory exposures …