Medicine and Health Sciences Commons^™

Human Schwann Cells In Vitro I. Nerve Tissue Processing, Pre-Degeneration, Isolation, And Culturing Of Primary Cells, Gabriela I. Aparicio, Paula V. Monje

Markey Cancer Center Faculty Publications

This paper presents versatile protocols to prepare primary human Schwann cell (hSC) cultures from mature peripheral nervous system tissues, including fascicles from long spinal nerves, nerve roots, and ganglia. This protocol starts with a description of nerve tissue procurement, handling, and dissection to obtain tissue sections suitable for hSC isolation and culturing. A description follows on how to disintegrate the nerve tissue by delayed enzymatic dissociation, plate the initial cell suspensions on a two-dimensional substrate, and culture the primary hSCs. Each section contains detailed procedures, technical notes, and background information to aid investigators in understanding and managing all steps. Some …

Phosphorylation Of Ahr By Plk1 Promotes Metastasis Of Luad Via Dio2-Th Signaling, Chaohao Li, Derek B. Allison, Daheng He, Fengyi Mao, Xinyi Wang, Piotr G. Rychahou, Ibrahim A. Imam, Yifan Kong, Qiongsi Zhang, Yanquan Zhang, Jinghui Liu, Ruixin Wang, Xiongjian Rao, Sai Wu, B. Mark Evers, Qing Shao, Chi Wang, Zhiguo Li, Xiaoqi Liu

Markey Cancer Center Faculty Publications

Metastasis of lung adenocarcinoma (LUAD) is a major cause of death in patients. Aryl hydrocarbon receptor (AHR), an important transcription factor, is involved in the initiation and progression of lung cancer. Polo-like kinase 1 (PLK1), a serine/threonine kinase, acts as an oncogene promoting the malignancy of multiple cancer types. However, the interaction between these two factors and their significance in lung cancer remain to be determined. In this study, we demonstrate that PLK1 phosphorylates AHR at S489 in LUAD, leading to epithelial-mesenchymal transition (EMT) and metastatic events. RNA-seq analyses reveal that type 2 deiodinase (DIO2) is responsible for EMT and …

Synthetic Contraceptive Hormones Occlude The Ability Of Nicotine To Reduce Ethanol Consumption In Ovary-Intact Female Rats, Erin E. Maher, Ashley M. White, Ashley Craig, Shailesh N. Khatri, Percell T. Kendrick Jr., Mary E. Matocha, Emma O. Bondy, Nikhil Pallem, Grace W. Breakfield, Madison Botkins, Olivia Sweatt, William C. Griffin, Brent A. Kaplan, Jessica Jane Weafer, Joshua S. Beckmann, Cassandra D. Gipson

Markey Cancer Center Faculty Publications

Rates of tobacco and alcohol use in women are rising, and women are more vulnerable than men to escalating tobacco and alcohol use. Many women use hormonal birth control, with the oral contraceptive pill being the most prevalent. Oral contraceptives contain both a progestin (synthetic progesterone) and a synthetic estrogen (ethinyl estradiol; EE) and are contraindicated for women over 35 years who smoke. Despite this, no studies have examined how synthetic contraceptive hormones impact this pattern of polysubstance use in females. To address this critical gap in the field, we treated ovary-intact female rats with either sesame oil (vehicle), the …

Machine Learning-Enabled Detection Of Unruptured Cerebral Aneurysms Improves Detection Rates And Clinical Care, Hyun-Woo Kim, Anjan Ballekere, Iman Ali, Sergio Salazar Marioni, Rania Abdelkhaleq, Arash Niktabe, Hussain Azeem, Ananya Iyyangar, Omri Segev, Orin Bibas, Dan Paz, Christopher J Love, Luca Giancardo, Sunil A Sheth

Faculty, Staff and Student Publications

Background: Unruptured cerebral aneurysms (UCAs) have a relatively low prevalence of ≈3%, but detection can prevent devastating consequences of subarachnoid hemorrhage. Here, we assess the performance of a machine learning algorithm to identify UCAs and determine whether routine use of the algorithm improves detection and patient care.

Methods: From a prospectively maintained multicenter registry across 8 certified stroke centers (1 comprehensive and 7 primary), we identified patients who underwent computed tomography angiography for evaluation of possible stroke from March 14, 2021, to November 31, 2021. A convolutional deep neural network (Viz ANEURYSM) trained to identify UCAs at least 4 mm …

Evaluating A Newborn Infant With Pancytopenia For Whim Syndrome, Shayan Sazdar, Thao Le, Nikita Raje

Posters

Introduction Warts, hypogammaglobulinemia, infections, and myelokathexis (WHIM) syndrome is a rare immunodeficiency caused by a gain-of-function mutation in CXCR4. A complication of WHIM syndrome is severe neutropenia, which can result in fatal bacterial infections. Given the rarity of the disease and variability of clinical presentations, WHIM syndrome can be difficult to diagnose and manage. Case Description A full term male infant was hospitalized for hypoxic ischemic encephalopathy after delivery. Physical exam was unremarkable. During his hospitalization, he was found to have progressive pancytopenia. Initial lymphocyte subsets showed a significant T cell lymphopenia of 1325 mm3 CD3+, 920 mm3 CD4+, 390 …

Evaluating Patients With Recurrent Fever And Elevated Igd, Thao Le, Nikita Raje

Posters

Introduction: Hyperimmunoglobulinemia D with periodic fever syndrome (HIDS) often presents within the first year of life. Symptoms include fevers, chills, headache, lymphadenopathy, and abdominal pain. Patients may be misdiagnosed as other autoinflammatory syndromes, resulting in delay of treatment. Case: A 16-year-old female presented for evaluation of recurrent fever. Her fevers started at two months of age, occurred one to two times a month, and were associated with headache, abdominal pain, and fatigue . At 18-months, she was diagnosed with periodic fever, aphthous stomatitis, pharyngitis, adenitis (PFAPA). She was treated with prednisone and an adenotonsillectomy but her fevers persisted. . At …

Understanding Macrophage Phagocytosis In Pediatric Leukemia, Molly Leyda, Jacqelyn Nemechek, John Szarejko, Fang Tao, Tykeem Manor, Douglas Myers, John M. Perry

Posters

Macrophages are a diverse and widespread type of innate immune cell which play an important role in homeostasis and defense. In a process called phagocytosis, macrophages engulf dying cells, foreign substances, and pathogens. Importantly, macrophages can present antigens from phagocytosed cells allowing them to initiate an adaptive immune response against remaining cells of the same type. Despite the immunosuppressive nature of the tumor microenvironment, macrophages often infiltrate tumors where they can either promote or inhibit cancer development, though the specific conditions influencing their pro- vs anti-cancer activity remain enigmatic. Recently, macrophages have been brought into the spotlight of immunotherapy research …

A Case Report Of A 48-Year-Old Female With Cauda Equina Syndrome In The Setting Of Disseminated Mrsa Infection, Aimee Lambeth, Sarah Eickmeyer, Stephanie Fortune

Posters

To present an interesting case of cauda equina syndrome due to disseminated MRSA, where inpatient EMG was useful in diagnosis and treatment planning.

Population-Specific Mutation Patterns In Breast Tumors From African American, European American, And Kenyan Patients, Wei Tang, Flora Zhang, Jung S. Byun, Shahin Sayed, Tiffany H. Dorsey, Harris G. Yfantis, Anuoluwapo Ajao, Huaitian Liu, Margaret Pichardo, Catherine Pichardo

Pathology, East Africa

Abstract: Women of African descent have the highest breast cancer mortality in the United States and are more likely than women from other population groups to develop an aggressive disease. It remains uncertain to what extent breast cancer in Africa is reminiscent of breast cancer in African American or European American patients. Here, we performed whole-exome sequencing of genomic DNA from 191 breast tumor and non-cancerous adjacent tissue pairs obtained from 97 African American, 69 European American, 2 Asian American, and 23 Kenyan patients. Our analysis of the sequencing data revealed an elevated tumor mutational burden in both Kenyan and …

Literature Review On The Effectiveness Of Poc Lactate Tests For Post-Operative Sepsis Discharge, Blaire Adler, Paarth Jain, Nathan Rim, Rachel Monane

Phase 1

Background: Every year in the USA, there are at least two million surgery-related infections; around 11% are derived from decompression spinal cord surgery. Generally, later detection of sepsis leads to significant burdens on the healthcare system; this manifests financially, temporally, and in poorer patient outcomes. “SepsisCheckToGo” is a toolkit aimed to minimize the number of sepsis complications in spinal surgery patients postoperatively; the kit would include a pulse oximeter, thermometer, and fingertip POC lactate tests to detect early sepsis signs after discharge and before the follow-up visit (which would occur around one month after initial discharge).

Methods: Our team did …

Coaching And Laissez-Faire Leadership Styles, Michelle C. Dziurgot Dds

The Journal of the Michigan Dental Association

Editor Dr. Michelle C. Dziurgot discusses the differences between coaching and laissez-faire leadership styles in dentistry. Coaching leaders motivate and educate their team members, fostering collaboration and improvement. In contrast, laissez-faire leaders take a more hands-off approach, allowing team members to make decisions independently. The author, a dentist, describes how she combines both styles in her practice, emphasizing the importance of educating and coaching team members while allowing them to take ownership of their roles. The editorial highlights the need for patient engagement in oral health care and the importance of coaching patients to take an active role in their …

Advocacy Spotlight: Important: State Issues Changes To Delegation Of Duties Rules, Neema Katibai Jd

The Journal of the Michigan Dental Association

The Michigan Board of Dentistry has introduced changes to the rules governing the delegation of duties to dental auxiliaries, including the reclassification of "dental assistants" (those not formally trained or licensed) as "unregistered dental auxiliaries" (UDAs). The classification Registered Dental Assistant (RDA) remains. Changes involve the supervision levels for specific tasks and guiding training UDAs for orthodontic duties. Notable changes include allowing UDAs to perform certain orthodontic tasks and expanding their scope of work to include duties such as taking impressions, polishing, and providing nutritional counseling. Dental professionals should know these changes and the supervision required for delegation to UDAs …

Staff Matters: How Can We Be More Creative With Our Benefits?, Jodi Schafer Sphr, Shrm-Scp

The Journal of the Michigan Dental Association

As open enrollment for health benefits approaches, this column discusses creative and cost-effective benefit offerings to attract and retain employees. The focus is on innovative benefits beyond traditional health, life insurance, and retirement plans. Suggestions include pet insurance, paid volunteer time, lunch and learn sessions, and flexible scheduling to accommodate employees' needs, particularly working parents and those with caregiving responsibilities. The importance of seeking employee input in benefit planning is emphasized for discovering practical, employee-desired options.

The Back Page Commentary: Let’S Talk About The Real World, Danielle Gehlert Dds

The Journal of the Michigan Dental Association

Dr. Danielle Gehlert, a private practitioner, writes that in the realm of continuing education for dental professionals, there's a need for a shift in focus from idealized, "sexy" cases to real-world patient scenarios. This commentary calls for seminars and programs emphasizing long-term restorative planning, including successes and failures. The practical considerations of patient financial limitations and willingness to engage in dental care must be integral to treatment plans. Real-world cases should reflect the diverse situations dental practitioners encounter and the ethical responsibility to provide affordable and effective treatment options.

Council Appeals For Member Input, Lance Plunkett Jd, Llm

The New York State Dental Journal

NYSDA's Council on Ethics is engaged in a review of the Association's Code of Ethics. It's a process it wants members to participate in.

Don't Throw Out The Employee Dentist Covenant-Not-To-Compete Baby With The Exploitive Employer Bathwater, Chester Gary Dds, Jd

The New York State Dental Journal

In our legal system's attempts to curb overreaching employer conduct, it must legislate strict controls, rather than overbroadly ban reasonable covenants not to compete that protect the dentist-patient relationship and incentivize dental practices to nurture one of society's most valuable assets: patient goodwill.

Use Of A 3d Intraoral Scanner For Prosthetic Rehabilitation Of Cocaine-Induced Oronasal Fistula, Anita Agarwal Bds, Dmd, Mph, Timothy Levine Dmd, Victor Badner Dmd, Mph

The New York State Dental Journal

A novel method using a 3D intraoral scanner (IOS) is used to overcome difficulties in obtaining conventional impressions to fabricate an obturator for a posteriorly located oronasal fistula secondary to chronic nasal cocaine use.

Verruciform Xanthoma, Khanh Trinh Dmd, Daniel Nassimi Dds, Daria Vaislyeva Dds, Scott Peters Dds

The New York State Dental Journal

Verruciform xanthoma (VX) is a benign epithelial proliferation typically found on the masticatory mucosa. VX usually affects patients in their 5 th to 6 th decade of life. VX is treated with simple excision with a low chance of recurrence after surgery.

Adenoid Ameloblastoma With Dentinoid, Eric Silver Dmd, Md, Stephen Roth Dds, Lydia Lam Dds, John Fantasia Dds, Steve Yusupov Dds, Md

The New York State Dental Journal

Adenoid ameloblastoma with dentinoid (AAD) is a rare benign neoplasm of odontogenic epithelium, with under 50 cases having been reported in the literature. AAD shares histopathological characteristics with both ameloblastomas and adenomatoid odontogenic tumors. They are unique because of dentinoid deposition without enamel. We present a case of AAD in the maxilla of a 13 year old. The patient was treated with left infrastructure maxillectomy and left partial infratemporal fossa dissection. This case reports on an extremely rare disease process in an uncommon location in a pediatric patient.

Mda Foundation: Mda Foundation Teams Up With Victors For Veterans Program, Anne Berquist

The Journal of the Michigan Dental Association

The MDA Foundation partners with the Victors for Veterans (V4V) program to provide dental care to impoverished veterans in Michigan, especially those with disabilities, homelessness, or limited income. The program, established in 2012, currently operates in the VINA Community Dental Center in Brighton, Michigan, providing free dental services to veterans in the region. The MDA Foundation supports this initiative with grants to secure clinic space, enabling 30 to 60 veterans to receive comprehensive dental care annually. Dental students at the University of Michigan School of Dentistry volunteer their time and skills and precept dentists oversee patient care.

Anterior Cervical Discectomy And Fusion After Presentation To The Acute Care Setting: A Comparison Of Post-Operative Outcomes, Alex Miller, James Whaley, Daniel Park

Conference Presentation Abstracts

Introduction: For patients presenting with cervical radiculopathy or cervical myelopathy, surgical treatment is typically pursued after discussion in the outpatient setting. Yet, there exists a group of patients presenting to the acute care setting with intractable pain or progressive neurologic deficits who are indicated for operative treatment outside of outpatient evaluation. Given the differences in initial management and pre-operative optimization, it is unclear if surgical and post-operative outcomes differ between these populations. Materials and Methods: This was a retrospective cohort study from an academic medical center. Surgical case logs from the institution’s electronic medical record were used to identify patients …

Corrigendum To 'Pressure-Mediated Reflection Spectroscopy Criterion Validity As A Biomarker Of Fruit And Vegetable Intake: A 2-Site Cross-Sectional Study Of 4 Racial Or Ethnic Groups' [J Nutr 2022;152:107-116], Stephanie B Jilcott Pitts, Nancy E Moran, Qiang Wu, Lisa Harnack, Neal E Craft, Neil Hanchard, Ronny Bell, Stacey G Moe, Nevin Johnson, Justice Obasohan, Pamela L Carr-Manthe, Melissa N Laska

Children’s Nutrition Research Center Staff Publications

No abstract provided.

Examining Potential Modifiers Of Human Skin And Plasma Carotenoid Responses In A Randomized Trial Of A Carotenoid-Containing Juice Intervention, Stephanie B Jilcott Pitts, Qiang Wu, Nancy E Moran, Melissa N Laska, Lisa Harnack

Children’s Nutrition Research Center Staff Publications

Background: Skin carotenoid measurements are emerging as a valid and reliable indicator of fruit and vegetable intake and carotenoid intake. However, little is known about the extent to which skin carotenoid responsivity to dietary changes differs based on demographic and physiologic characteristics.

Objectives: This study examined potential effect modifiers of skin carotenoid and plasma carotenoid responses to a carotenoid-rich juice intervention.

Methods: We leveraged data from 2 arms of a 3-site randomized controlled trial of a carotenoid-containing juice intervention (moderate dose = 6 ounces juice, 4 mg total carotenoids/d, high dose = 12 ounces juice, 8 mg total carotenoids/d) (n …

N Y State Dent J November 2023

The New York State Dental Journal

In the November 2023 issue, the reader will find the following feature articles:

• Verruciform Xanthoma
• Use of a 3D Intraoral Scanner for Prosthetic Rehabilitation of Cocaine-Induced Oronasal Fistula
• Adenoid Ameloblastoma with Dentinoid

This issue includes regular columns with regional news impacting the New York membership including: editorial and perspectives columns, legal, association activities, component news, continuing education opportunities, and classifieds.

U-M’S Sindecuse Museum Of Dentistry: A Treasure In Our Own Backyard, Tamara Barnes

The Journal of the Michigan Dental Association

Discover the University of Michigan's Gordon H. Sindecuse Museum of Dentistry, a captivating journey through dental history. Founded in 1992 with contributions from Dr. Gordon Sindecuse, a 1921 dental school graduate, this museum offers a treasure trove of over 15,000 artifacts from the 18th to the 21st century. The main exhibits depict the evolution of dentistry from its humble origins, showcasing groundbreaking innovations, including electrification, anesthesia, and dental hygiene. Special exhibits explore dental practices' social and cultural aspects, such as teeth modifications for self-expression. The museum embraces technology with QR codes and offers diverse programming, making it an educational and …

High Acceptability, Convenience And Reduced Carbon Emissions Of Tele-Neurology Outpatient Services At A Regional Referral Centre In Kenya, Fazal Yakub, Jasmit Shah, Dilraj Sokhi

Internal Medicine, East Africa

Background: There is severe shortage of neurologists in sub-Saharan Africa. Tele-neurology consultations (TNC) can bridge this service gap, but there is very little published evidence on TNC in our setting, which we addressed through our study.

Methods: We prospectively enrolled patients at our neurology outpatients from October 2020 to October 2021. We administered a post-TNC questionnaire which captured satisfaction/acceptability using Likert scales. A sub-group of participants who also did in-person consultations (IPC) were additionally administered post-IPC questionnaires. Statistical comparisons were made using the paired student t-test, and descriptive data expressed as median (inter-quartile range).

Results: From 219 enrolled patients, 66.7% …

The Pattern Of Admission, Clinical Characteristics, And Outcomes Among Patients Admitted To The Intensive Care Unit Of A Tertiary Hospital In Tanzania: A 5-Year Retrospective Review, Nadeem Kassam, Philip Adebayo, Iris Martin Matei, Eric Aghan, Samina Somji, Samwel Paul Kadelya, Yasson Abha, Frank Elton Swai, Mangaro Mabusi, Kamran Hamid

Internal Medicine, East Africa

Introduction: Despite the implementation of complex interventions, ICU mortality remains high and more so in developing countries. The demand for critical care in Sub-Saharan Africa is more than ever before as the region experiences a double burden of rising rates of non-communicable diseases (NCD) in the background battle of combating infectious diseases. Limited studies in Tanzania have reported varying factors associated with markedly high rates of ICU mortality. Investigating the burden of ICU care remains crucial in providing insights into the effectiveness and challenges of critical care delivery.

Material and Methods: A single-center retrospective study that reviewed records of all …

Captive Samples Are Not The Answer To Survey Response Rates., Bryce A Ringwald

Medical Education Articles

No abstract provided.

Large Vessel Occlusion Prediction Scale Thresholds That Are Sensitive For Dawn Trial Patients., Kevin J Keenan, Wade S Smith, Ashutosh P Jadhav, Diogo C Haussen, Ronald F Budzik, Alain Bonafé, Parita Bhuva, Dileep R Yavagal, Marc Ribò, Christophe Cognard, Ricardo A Hanel, Ameer E Hassan, Cathy A Sila, Jeffrey L Saver, David S Liebeskind, Tudor G Jovin, Raul G Nogueira

Neuroscience Articles

BACKGROUND: Large vessel occlusion (LVO) prediction scales are used to triage prehospital suspected stroke patients with a high probability of LVO stroke to endovascular therapy centers. The sensitivities of these scales in the 6-to-24-h time window are unknown. Higher scale score thresholds are typically less sensitive and more specific. Knowing the highest scale score thresholds that remain sensitive could inform threshold selection for clinical use. Sensitivities may also vary between left and right-sided LVOs.

METHODS: LVO prediction scale scores were retrospectively calculated using the National Institutes of Health Stroke Scale (NIHSS) scores of patients enrolled in the DAWN Trial. All …

Transposable Elements Are Co-Opted As Oncogenic Regulatory Elements By Lineage-Specific Transcription Factors In Prostate Cancer, Giacomo Grillo, Tina Keshavarzian, Simon Linder, Christopher Arlidge, Lisanne Mout, Ankita Nand, Mona Teng, Aditi Qamra, Stanley Zhou, Ken J Kron, Alex Murison, James R Hawley, Michael Fraser, Theodorus H Van Der Kwast, Ganesh V Raj, Housheng Hansen He, Wilbert Zwart, Mathieu Lupien

Faculty, Staff and Student Publications

Transposable elements hold regulatory functions that impact cell fate determination by controlling gene expression. However, little is known about the transcriptional machinery engaged at transposable elements in pluripotent and mature versus oncogenic cell states. Through positional analysis over repetitive DNA sequences of H3K27ac chromatin immunoprecipitation sequencing data from 32 normal cell states, we report pluripotent/stem and mature cell state–specific “regulatory transposable elements.” Pluripotent/stem elements are binding sites for pluripotency factors (e.g., NANOG, SOX2, OCT4). Mature cell elements are docking sites for lineage-specific transcription factors, including AR and FOXA1 in prostate epithelium. Expanding the analysis to prostate tumors, we identify a …