Medicine and Health Sciences Commons^™

Integrated Mrna Sequence Optimization Using Deep Learning, Haoran Gong, Jianguo Wen, Ruihan Luo, Yuzhou Feng, Jingjing Guo, Hongguang Fu, Xiaobo Zhou

Faculty, Staff and Student Publications

The coronavirus disease of 2019 pandemic has catalyzed the rapid development of mRNA vaccines, whereas, how to optimize the mRNA sequence of exogenous gene such as severe acute respiratory syndrome coronavirus 2 spike to fit human cells remains a critical challenge. A new algorithm, iDRO (integrated deep-learning-based mRNA optimization), is developed to optimize multiple components of mRNA sequences based on given amino acid sequences of target protein. Considering the biological constraints, we divided iDRO into two steps: open reading frame (ORF) optimization and 5' untranslated region (UTR) and 3'UTR generation. In ORF optimization, BiLSTM-CRF (bidirectional long-short-term memory with conditional random …

Ontologies Applied In Clinical Decision Support System Rules: Systematic Review, Xia Jing, Hua Min, Yang Gong, Paul Biondich, David Robinson, Timothy Law, Christian Nohr, Arild Faxvaag, Lior Rennert, Nina Hubig, Ronald Gimbel

Faculty, Staff and Student Publications

BACKGROUND: Clinical decision support systems (CDSSs) are important for the quality and safety of health care delivery. Although CDSS rules guide CDSS behavior, they are not routinely shared and reused.

OBJECTIVE: Ontologies have the potential to promote the reuse of CDSS rules. Therefore, we systematically screened the literature to elaborate on the current status of ontologies applied in CDSS rules, such as rule management, which uses captured CDSS rule usage data and user feedback data to tailor CDSS services to be more accurate, and maintenance, which updates CDSS rules. Through this systematic literature review, we aim to identify the frontiers …

Impact Of Adherence To Procalcitonin Antibiotic Prescribing Guideline Recommendations For Low Procalcitonin Levels On Antibiotic Use, Brian E Malley, Jonathan G Yabes, Elizabeth Gimbel, Chung-Chou H Chang, Donald M Yealy, Michael J Fine, Derek C Angus, David T Huang, Proact Investigators

Faculty, Staff and Student Publications

BACKGROUND: The Procalcitonin Antibiotic Consensus Trial (ProACT) found provision of a procalcitonin antibiotic prescribing guideline to hospital-based clinicians did not reduce antibiotic use. Possible reasons include clinician reluctance to follow the guideline, with an observed 64.8% adherence rate. In this study we sought to determine the threshold adherence rate for reduction in antibiotic use, and to explore opportunities to increase adherence.

METHODS: This study is a retrospective analysis of ProACT data. ProACT randomized 1656 patients presenting to 14 U.S. hospitals with suspected lower respiratory tract infection to usual care or provision of procalcitonin assay results and an antibiotic prescribing guideline …

Assessing The Efficacy Of A Meditation Intervention On Dispositional Mindfulness Among Medical Students And Resident Physicians, Kelsey Stefan, Michael Villeneuve, Varun Konanki, Erica Rarity, Rahul Mhaskar, Liwei Chen, Amelia Phillips

Journal of Wellness

Introduction: Dispositional mindfulness is the act of paying attention to one’s thoughts and feelings with an open and non-judgemental attitude. Medical students and resident physicians are under immense pressure and stress daily, and they need effective tools to help increase their dispositional mindfulness. This project examined the impact of a meditation intervention on medical students’ dispositional mindfulness.

Methods: In 2020, medical students and resident physicians at the University of South Florida were given the option to practice daily meditation for thirty days; time spent on the practice varied depending on comfort with the practice. Dispositional mindfulness levels were assessed using …

Pgc-1Α Senses The Cbc Of Pre-Mrna To Dictate The Fate Of Promoter-Proximally Paused Rnapii, Xavier Rambout, Hana Cho, Roméo Blanc, Qing Lyu, Joseph M Miano, Joe V Chakkalakal, Geoffrey M Nelson, Hari K Yalamanchili, Karen Adelman, Lynne E Maquat

Children’s Nutrition Research Center Staff Publications

PGC-1α is well established as a metazoan transcriptional coactivator of cellular adaptation in response to stress. However, the mechanisms by which PGC-1α activates gene transcription are incompletely understood. Here, we report that PGC-1α serves as a scaffold protein that physically and functionally connects the DNA-binding protein estrogen-related receptor α (ERRα), cap-binding protein 80 (CBP80), and Mediator to overcome promoter-proximal pausing of RNAPII and transcriptionally activate stress-response genes. We show that PGC-1α promotes pausing release in a two-arm mechanism (1) by recruiting the positive transcription elongation factor b (P-TEFb) and (2) by outcompeting the premature transcription termination complex Integrator. Using mice …

Semi-Quantitative Detection Of Pseudouridine Modifications And Type I/Ii I/Ii Hypermodifications In Human Mrnas Using Direct Long-Read Sequencing, Sepideh Tavakoli, Mohammad Nabizadeh, Amr Makhamreh, Howard Gamper, Caroline A Mccormick, Neda K Rezapour, Ya-Ming Hou, Meni Wanunu, Sara H Rouhanifard

Department of Biochemistry and Molecular Biology Faculty Papers

Here, we develop and apply a semi-quantitative method for the high-confidence identification of pseudouridylated sites on mammalian mRNAs via direct long-read nanopore sequencing. A comparative analysis of a modification-free transcriptome reveals that the depth of coverage and specific k-mer sequences are critical parameters for accurate basecalling. By adjusting these parameters for high-confidence U-to-C basecalling errors, we identify many known sites of pseudouridylation and uncover previously unreported uridine-modified sites, many of which fall in k-mers that are known targets of pseudouridine synthases. Identified sites are validated using 1000-mer synthetic RNA controls bearing a single pseudouridine in the center position, demonstrating systematic …

Control Of Craniofacial Development By The Collagen Receptor, Discoidin Domain Receptor 2, Fatma F Mohamed, Chunxi Ge, Shawn A Hallett, Alec C Bancroft, Randy T Cowling, Noriaki Ono, Abdul-Aziz Binrayes, Barry Greenberg, Benjamin Levi, Vesa M Kaartinen, Renny T Franceschi

Faculty, Staff and Student Publications

Development of the craniofacial skeleton requires interactions between progenitor cells and the collagen-rich extracellular matrix (ECM). The mediators of these interactions are not well-defined. Mutations in the discoidin domain receptor 2 gene (DDR2), which encodes a non-integrin collagen receptor, are associated with human craniofacial abnormalities, such as midface hypoplasia and open fontanels. However, the exact role of this gene in craniofacial morphogenesis is not known. As will be shown, Ddr2-deficient mice exhibit defects in craniofacial bones including impaired calvarial growth and frontal suture formation, cranial base hypoplasia due to aberrant chondrogenesis and delayed ossification at growth plate …

A Training Curriculum For An Mhealth Supported Peer Counseling Program To Promote Exclusive Breastfeeding In Rural India, Roopa M. Bellad, Niranjana S. Mahantshetti, Umesh S. Charantimath, Tony Ma, Yukiko Washio, Vanessa L. Short, Katie Chang, Parth Lalakia, Frances J. Jaeger, Patricia J. Kelly, Geetanjali Mungarwadi, Chandrashekar C. Karadiguddi, Shivaprasad S. Goudar, Richard J. Derman

Department of Pediatrics Faculty Papers

BACKGROUND: Despite strong evidence about the benefits of exclusive breastfeeding, that is the baby receiving only breast milk, no other foods or liquids, rates have remained relatively unchanged over the past two decades in low- and middle-income countries. One strategy for increasing exclusive breastfeeding is through community-based programs that use peer counselors for education and support. The use of mobile health applications is also gaining increasing applicability in these countries. Minimal information is available about training peer counselors in the use of mobile technologies to support exclusive breastfeeding. The present article describes our curriculum in the state of Karnataka, India …

Circadian Rhythms As Modulators Of Brain Health During Development And Throughout Aging, Rachel Van Drunen, Kristin Eckel-Mahan

Faculty, Staff and Student Publications

The circadian clock plays a prominent role in neurons during development and throughout aging. This review covers topics pertinent to the role of 24-h rhythms in neuronal development and function, and their tendency to decline with aging. Pharmacological or behavioral modification that augment the function of our internal clock may be central to decline of cognitive disease and to future chronotherapy for aging-related diseases of the central nervous system.

Long-Term Bidirectional Association Between Asthma And Attention Deficit Hyperactivity Disorder: A Big Data Cohort Study, Hye Jin Park, Young Hyeon Kim, Da Ye Na, Seong Won Jeong, Min Gyu Lee, Jong-Hwan Lee, Yun Na Yang, Min Gu Kang, Sang Woo Yeom, Jong Seung Kim

Faculty, Staff and Student Publications

BACKGROUND: Previous studies have argued that attention deficit hyperactivity disorder (ADHD) is associated with asthma. However, reliable evidence to verify this association has not yet been reported.

OBJECTIVES: To investigate the bidirectional association between asthma and ADHD through a 12-year big data cohort study.

METHODS: The independent variable group was extracted from 3.5 million individuals randomly sampled by the National Health Insurance Service (NHIS). In Study 1, the incidence of ADHD according to asthma was evaluated, while in Study 2, the incidence of asthma according to ADHD was analyzed. Propensity score (PS) matching with several variables was used to obtain …

A Comprehensive Assessment Of Cell Type-Specific Differential Expression Methods In Bulk Data, Guanqun Meng, Wen Tang, Emina Huang, Ziyi Li, Hao Feng

Faculty, Staff and Student Publications

Accounting for cell type compositions has been very successful at analyzing high-throughput data from heterogeneous tissues. Differential gene expression analysis at cell type level is becoming increasingly popular, yielding biomarker discovery in a finer granularity within a particular cell type. Although several computational methods have been developed to identify cell type-specific differentially expressed genes (csDEG) from RNA-seq data, a systematic evaluation is yet to be performed. Here, we thoroughly benchmark six recently published methods: CellDMC, CARseq, TOAST, LRCDE, CeDAR and TCA, together with two classical methods, csSAM and DESeq2, for a comprehensive comparison. We aim to systematically evaluate the performance …

Ccsynergy: An Integrative Deep-Learning Framework Enabling Context-Aware Prediction Of Anti-Cancer Drug Synergy, Sayed-Rzgar Hosseini, Xiaobo Zhou

Faculty, Staff and Student Publications

Combination therapy is a promising strategy for confronting the complexity of cancer. However, experimental exploration of the vast space of potential drug combinations is costly and unfeasible. Therefore, computational methods for predicting drug synergy are much needed for narrowing down this space, especially when examining new cellular contexts. Here, we thus introduce CCSynergy, a flexible, context aware and integrative deep-learning framework that we have established to unleash the potential of the Chemical Checker extended drug bioactivity profiles for the purpose of drug synergy prediction. We have shown that CCSynergy enables predictions of superior accuracy, remarkable robustness and improved context generalizability …

Editorial: A Surgeon’S Framework For The Unplanned Intraoperative Consultation, Sunjay Kumar, Talar Tatarian, Francesco Palazzo

Department of Surgery Faculty Papers

Surgeons during their career will likely be called in to assist or offer advice with an unanticipated intraoperative event or finding. Yet, there is no practical framework of how to respond to these consults nor is there any formal training in this area.

Review of the limited literature and expert senior opinion can help explain the ethical components involved but do not address some of the practical aspects that the consulting surgeon may need to confront when responding to an unplanned intraoperative consultation.

In this manuscript we present a selected review of the available literature on the topic, establish some …

Ultrasound Assessment Of Spastic Muscles In Ambulatory Chronic Stroke Survivors Reveals Function-Dependent Changes, Javier González-Buonomo, Alexander H Pham, Jaskiran Ghuman, Aila Malik, Nuray Yozbatiran, Gerard E Francisco, Walter R Frontera, Sheng Li

Faculty, Staff and Student Publications

OBJECTIVE: To correlate ultrasound characteristics of spastic muscles with clinical and functional measurements in chronic stroke survivors.

METHODS: Ultrasound assessment and clinical and functional assessments were performed in 28 ambulatory stroke survivors (12 females, mean age 57.8 ± 11.8 years, 76 ± 45 months after stroke).

RESULTS: Muscle thickness in the affected side was decreased compared with the contralateral side (p < 0.001). The decrease was more evident in the upper limb muscles. On the affected side, the modified Heckmatt scale score was lowest (closer to normal) in the rectus femoris (RF) muscle compared with other muscles (biceps brachii (BB), flexor carpi ulnaris (FCU) and medial gastrocnemius (MG)). Muscle thickness and echogenicity of spastic muscles did not correlate with spasticity, as measured with the modified Ashworth scale (MAS), Fugl-Meyer motor assessment scores, age, or time since stroke. There was a significant negative correlation between grip strength and percentage decrease in muscle thickness for the spastic FCU muscle (r = -0.49, p = 0.008). RF muscle thickness correlated with ambulatory function (Timed Up and Go test (r = 0.44, p = 0.021) and 6-metre walk test (r = 0.41, p = 0.032)). There was no significant correlation between echogenicity and functional assessments Conclusion: Ambulatory chronic stroke survivors had function-dependent changes in muscle thickness on the affected side. Muscle thickness and echogenicity of spastic muscles did not correlate with spasticity, Fugl-Meyer motor assessment scores, age, or time since stroke.

Cortical Connectomic Mediations On Gamma Band Synchronization In Schizophrenia, Xiaoming Du, Stephanie Hare, Ann Summerfelt, Bhim M Adhikari, Laura Garcia, Wyatt Marshall, Peng Zan, Mark Kvarta, Eric Goldwaser, Heather Bruce, Si Gao, Hemalatha Sampath, Peter Kochunov, Jonathan Z Simon, L Elliot Hong

Faculty, Staff and Student Publications

Aberrant gamma frequency neural oscillations in schizophrenia have been well demonstrated using auditory steady-state responses (ASSR). However, the neural circuits underlying 40 Hz ASSR deficits in schizophrenia remain poorly understood. Sixty-six patients with schizophrenia spectrum disorders and 85 age- and gender-matched healthy controls completed one electroencephalography session measuring 40 Hz ASSR and one imaging session for resting-state functional connectivity (rsFC) assessments. The associations between the normalized power of 40 Hz ASSR and rsFC were assessed via linear regression and mediation models. We found that rsFC among auditory, precentral, postcentral, and prefrontal cortices were positively associated with 40 Hz ASSR in …

Urine Cell-Free Dna Multi-Omics To Detect Mrd And Predict Survival In Bladder Cancer Patients, Pradeep S Chauhan, Alexander Shiang, Irfan Alahi, Wenjia Feng, Cayce Nawaf, Kevin Chen, Peter K Harris, Faridi Qaium, Casey Hatscher, Anna Antiporda, Lindsey Brunt, Lindsey R Mayer, Brian C Baumann, Eric H Kim, Melissa A Reimers, Zachary L Smith, Aadel A Chaudhuri, Et Al.

2020-Current year OA Pubs

Circulating tumor DNA (ctDNA) sensitivity remains subpar for molecular residual disease (MRD) detection in bladder cancer patients. To remedy this problem, we focused on the biofluid most proximal to the disease, urine, and analyzed urine tumor DNA in 74 localized bladder cancer patients. We integrated ultra-low-pass whole genome sequencing (ULP-WGS) with urine cancer personalized profiling by deep sequencing (uCAPP-Seq) to achieve sensitive MRD detection and predict overall survival. Variant allele frequency, inferred tumor mutational burden, and copy number-derived tumor fraction levels in urine cell-free DNA (cfDNA) significantly predicted pathologic complete response status, far better than plasma ctDNA was able to. …

Pharmacological Management Of Disease By Overcoming Structural Adversity, Richard N Sifers

Center on Aging Staff Publications

In this issue of Cell Chemical Biology, Sun et al. utilize computational and protein expression analyses, plus pharmacological proteostasis network activation, to simultaneously correct two genetic diseases linked to a single protein rather than modify the responsible DNA.

Psychosocial Measures And Outcomes Among Caregivers Of Children With Tracheostomies: A Systematic Review, Darlene E Acorda, Jennifer N Brown, Elton M Lambert, Karen Divalerio Gibbs

Library Staff Publications

OBJECTIVE: Children with tracheostomies have complex medical issues that require long-term technology dependence and continuous medical care at home. Parents of tracheostomy-dependent children often assume the majority of their child's home care leading to a shift in family dynamics and a decrease in caregiver quality of life. This systematic review sought to identify instruments to measure caregiver psychosocial outcomes after their child's tracheostomy and report on the findings.

DATA SOURCES: A systematic review was performed using Medline, CINAHL, and EMBASE.

REVIEW METHODS: Studies that evaluated psychosocial outcomes in caregivers of tracheostomy-dependent children were included.

RESULTS: We screened a total of …

Virome Analysis And Association Of Positive Coxsackievirus B Serology During Pregnancy With Congenital Heart Disease, Mathieu Garand, Susie S Y Huang, Lisa S Goessling, Fei Wan, Donna A Santillan, Mark K Santillan, Anoop Brar, Todd N Wylie, Kristine M Wylie, Pirooz Eghtesady

2020-Current year OA Pubs

BACKGROUND: We have previously shown coxsackievirus B (CVB) to be a potent inducer of congenital heart disease (CHD) in mice. The clinical relevance of these findings in humans and the roles of other viruses in the pathogenesis of CHD remain unknown.

METHODS: We obtained plasma samples, collected at all trimesters, from 89 subjects (104 pregnancies), 73 healthy controls (88 pregnancies), and 16 with CHD-affected birth (16 pregnancies), from the Perinatal Family Tissue Bank (PFTB). We performed CVB IgG/IgM serological assays on plasma. We also used ViroCap sequencing and PCR to test for viral nucleic acid in plasma, circulating leukocytes from …

Semantic Wikis As Flexible Database Interfaces For Biomedical Applications, Marco Falda, Manfredo Atzori, Maurizio Corbetta

2020-Current year OA Pubs

Several challenges prevent extracting knowledge from biomedical resources, including data heterogeneity and the difficulty to obtain and collaborate on data and annotations by medical doctors. Therefore, flexibility in their representation and interconnection is required; it is also essential to be able to interact easily with such data. In recent years, semantic tools have been developed: semantic wikis are collections of wiki pages that can be annotated with properties and so combine flexibility and expressiveness, two desirable aspects when modeling databases, especially in the dynamic biomedical domain. However, semantics and collaborative analysis of biomedical data is still an unsolved challenge. The …

Socio-Demographic Determinants Of Non-Utilisation Of Antenatal Care Services By Women In The Northern Region Of Nigeria, Abubakar Yakubu Abbani, Yothin Sawangdee, Olusola Akintoye Omisakin, Maretalinia Maretalinia

Journal of Health Research

Background: A large proportion of women in the northern region of Nigeria do not utilise antenatal care (ANC) services. As a result, the region has the worst maternal and child health indicators. This study aims to identify the socio-demographic determinants of the non-utilisation of ANC services by pregnant women to provide evidence for policymakers to base decisions towards addressing the problem.

Methods: Data from the 2018 Nigeria Demographic and Health Survey was used with a sample of 14,421 women with a pregnancy history. Descriptive, bivariate, and hierarchical regression analyses were applied to the data using STATA software version 15.

Results: …

Online Dispositional Flow Enhancement Training For Chinese Recreational Runners: A Single-Group Mixed-Method Evaluation, Lijuan Yang, Rajitha Menon

Journal of Health Research

Background: There are limited studies examining the effectiveness of Mindful Sport Performance Enhancement (MSPE) on dispositional flow enhancement in the recreational population. This is the first empirical evaluation of an online 4-week MSPE designed to enhance dispositional flow in Chinese recreational runners.

Method: In this mixed-method study, a convergent design approach was adopted to examine the acceptability and effectiveness of the 4-week online MSPE in Chinese recreational runners (N = 41). The quantitative strand was designed with a single-group pre-test and post-test model in which the dispositional flow was assessed at 2-time points (i.e., pre and post) using the Chinese …

Living Healthier: A Grounded Theory Of Older Adults With Prehypertension, Junjira Seesawang, Pulawit Thongtang

Journal of Health Research

Background: To prevent hypertension and long-term risk of cardiovascular events, older adults with prehypertension have to adhere to preventive behaviors. Little is known about the experience of adherence to preventive behavior among older adults with prehypertension.

Method: A constructivist grounded theory approach was used to develop a model explaining the process of adherence to preventive behavior among older adults with prehypertension living in Thailand. Participants were recruited using purposive and theoretical samplings from two primary health care units in Western Thailand. Semi-structured interviews were conducted with 26 participants. All interview data collection was recorded, transcribed, and analyzed simultaneously. Initial and …

Health Workers’ Knowledge, Attitudes, And Practices On Reporting Adverse Events Following Immunization (Aefi) In Nigeria: A Cross-Sectional Study, Itse Olaoye, Fatiregun Akinola, Kyriaki Myrissa, Eirini Kelaiditi, Aniebet Ekong, Tsemaye Jacdonmi, Famokun Gboyega, Stephen Fagbemi

Journal of Health Research

Background: Reporting of adverse events following immunization (AEFI) is not the sole responsibility of health workers providing routine immunization services, but also of health workers providing clinical treatments and working in other health units. This study aimed to assess health workers’ level of knowledge as well as reporting attitudes and practices on AEFI in Ondo State, Nigeria.

Method: A cross‑sectional survey using a convenience sampling method was used to recruit Primary Health Care workers in four Local Government Areas (LGAs). A self‑reported questionnaire was adapted from a previous study and used to assess knowledge, attitudes, and practices on AEFI.

Results: …

Health Problems, Healthcare Service Utilisation And Its Associated Factors Among The Homeless In Malaysia: A Systematic Review, Fadly Syah Arsad, Rozita Hod, Mohd Rizal Abdul Manaf

Journal of Health Research

Background: Homelessness is a growing concern since it affects many individuals worldwide. Homeless people are vulnerable to health problems but tend to underutilise health services. Despite being a worldwide concern, there is little research on health issues and healthcare utilisation among homeless people in Malaysia.

Method: Three databases were used to search for relevant articles: Scopus, Web of Science, and PubMed. Studies published between 2011 and 2021 that examined the health problems and health service utilisation among homeless adults in Malaysia were considered. Manual searches in reference lists and grey literature were also performed to add reports. Data were extracted …

Hand Hygiene Behaviors Associated With Peritoneal Dialysis-Related Infection, Piyaporn Towannang, Aurawamon Sriyuktasuth, Doungrut Wattanakitkrileart, Talerngsak Kanjanabuch

Journal of Health Research

Background: Hand hygiene (HH) is an effective strategy to prevent infections. This study aimed to explore the association between HH behavior among peritoneal dialysis (PD) patients or caregivers and PD-related infection.

Method: A single-center, cross-sectional study was conducted among PD patients and primary caregivers of totally-dependent PD patients. Data were collected through a participant information form and three different questionnaires about knowledge of HH, facilities for HH, and HH behavior. Documentation of PD-related infections were obtained from patients’ medical records during the last 12 months. Descriptive statistics and binary logistic regression were used to analyze the data.

Results: A total …

Effectiveness Of Interactive Text Message Service At The Thailand National Quitline: A Randomized Controlled Trial, Suwimon Rojnawee, Chollada Jongsomjitt, Jintana Yunibhand

Journal of Health Research

Background: Automatic instant messaging systems are currently a well-known smoking reduction tool for public health. However, smokers’ engagement is lacking, as many prefer to communicate with experts during the quitting period so they can receive advice on dealing with the consequences of nicotine abstinence. Therefore, this study aimed to assess the effectiveness of an interactive text messaging service at the Thailand National Quitline.

Method: The study was conducted as a randomized controlled trial. A total of 314 smokers were eligible, 153 of whom were randomly assigned to receive two-way short messages encouraging them to quit smoking. After the date of …

Effect Of The Culturally Tailored Diabetes Prevention Program For At-Risk Thai Muslim People In Semi-Urban Areas, Yuparat Odglun, Kitti Sranacharoenpong, Nattvara Nirdnoy

Journal of Health Research

Background: Lifestyle intervention is effective in preventing type 2 diabetes mellitus (T2DM). The efficacy of intervention components across different sociocultural settings is relevant for real world implementation. Thus, this study aimed to evaluate the effectiveness of a culturally tailored diabetes prevention program (CTDPP) for at-risk Muslim people in Thailand.

Method: The CTDPP was developed and culturally adapted based on formative research. Participants at risk for diabetes were recruited and randomly assigned to either the intervention group (n=60) or the control group (n=59). Study group subjects participated in the CTDPP for 3 months. Control group subjects received only routine self-care recommendations …

Implicit Bias Training In The Student Nurse Practitioner’S Curriculum, Macdana Selecon

DNP Qualifying Manuscripts

Background: Implicit bias (IB) training is not provided in all graduate nursing programs. As a result, graduate nursing students are not prepared to address poor health outcomes related to healthcare biases. This literature review examines the evidence for effective IB training methodologies.

Methods: CINAHL, Fusion, PubMed, Google Scholar, and Cochrane Database of Systematic Reviews were searched for peer-reviewed articles published between 2010 and 2022. The identified studies were critically appraised using the Johns Hopkins Nursing Evidence-Based Practice Level and Quality Guide Tool.

Results: Six articles were identified as eligible. Five studies implemented implicit bias training for graduate nursing students and …

Prescription Drug Retail Sales In The Mountain West, Caren Royce Yap, Caitlin J. Saladino, William E. Brown Jr.

Health

This fact sheet synthesizes data on prescription drug retail sales in the Mountain West (Arizona, Colorado, Nevada, New Mexico, and Utah). "Retail Sales for Prescription Drugs Filled at Pharmacies by Payer," a 2019 report by the Kaiser Family Foundation, includes data on the amount of retail sales for prescription drugs made in each state by dollar amount, along with the method of coverage, including commercial, Medicare, Medicaid and cash payment.