Medicine and Health Sciences Commons^™

Circulating Plasma Exosomal Proteins Of Either Shiv-Infected Rhesus Macaque Or Hiv-Infected Patient Indicates A Link To Neuropathogenesis, Partha K. Chandra, Stephen E. Braun, Sudipa Maity, Jorge A. Castorena-Gonzalez, Hogyoung Kim, Jeffrey G. Shaffer, Sinisa Cikic, Ibolya Rutkai, Jia Fan, Jessie J. Guidry, David K. Worthylake, Chenzhong Li, Asim B. Abdel-Mageed, David W. Busija

School of Medicine Faculty Publications

Despite the suppression of human immunodeficiency virus (HIV) replication by combined antiretroviral therapy (cART), 50–60% of HIV-infected patients suffer from HIV-associated neurocognitive disorders (HAND). Studies are uncovering the role of extracellular vesicles (EVs), especially exosomes, in the central nervous system (CNS) due to HIV infection. We investigated links among circulating plasma exosomal (crExo) proteins and neuropathogenesis in simian/human immunodeficiency virus (SHIV)-infected rhesus macaques (RM) and HIV-infected and cART treated patients (Patient-Exo). Isolated EVs from SHIV-infected (SHIV-Exo) and uninfected (CTL-Exo) RM were predominantly exosomes (particle size < 150 nm). Proteomic analysis quantified 5654 proteins, of which 236 proteins (~4%) were significantly, differentially expressed (DE) between SHIV-/CTL-Exo. Interestingly, different CNS cell specific markers were abundantly expressed in crExo. Proteins involved in latent viral reactivation, neuroinflammation, neuropathology-associated interactive as well as signaling molecules were expressed at significantly higher levels in SHIV-Exo than CTL-Exo. However, proteins involved in mitochondrial biogenesis, ATP production, autophagy, endocytosis, exocytosis, and cytoskeleton organization were significantly less expressed in SHIV-Exo than CTL-Exo. Interestingly, proteins involved in oxidative stress, mitochondrial biogenesis, ATP production, and autophagy were significantly downregulated in primary human brain microvascular endothelial cells exposed with HIV+/cART+ Patient-Exo. We showed that Patient-Exo significantly increased blood–brain barrier permeability, possibly due to loss of platelet endothelial cell adhesion molecule-1 protein and actin cytoskeleton structure. Our novel findings suggest that circulating exosomal proteins expressed CNS cell markers—possibly associated with viral reactivation and neuropathogenesis—that may elucidate the etiology of HAND.

Neural Mechanisms Of Language Development In Infancy, Scott Huberty, Christian O'Reilly, Virginia Carter Leno, Mandy Steiman, Sara J. Webb, Mayada Elsabbagh, The Basis Team

Publications

Understanding the neural processes underpinning individual differences in early language development is of increasing interest, as it is known to vary in typical development and to be quite heterogeneous in neurodevelopmental conditions. However, few studies to date have tested whether early brain measures are indicative of the developmental trajectory of language, as opposed to language outcomes at specific ages. We combined recordings from two longitudinal studies, including typically developing infants without a family history of autism, and infants with increased likelihood of developing autism (infant-siblings) (N = 191). Electroencephalograms (EEG) were recorded at 6 months, and behavioral assessments at …

A Case Of Obstructed Left Diaphragmatic Hernia, Shikha Agarwal Dr.

Digital Journal of Clinical Medicine

Diaphragmatic hernia is protrusion of abdominal viscera into the chest cavity. Most commonly seen due to congenital defects in the diaphragm, hence causing herniation of abdominal contents including stomach, bowel, pancreas, omentum. Acquired diaphragmatic hernia can be secondary to injury to diaphragm. Patient presents with respiratory distress, due to reduced lung expansion. Early correlation with radiological scans is required for appropriate timely management, to prevent further deterioration of condition.

Development And Implementation Of Telemetry Devices To Identify And Characterize Sources Of Intraocular Pressure Variability In Rats, Christina M. Nicou

USF Tampa Graduate Theses and Dissertations

Eye health depends partially on intraocular pressure (IOP) as abnormal levels can lead to ocular tissue damage. Glaucoma is a neurodegenerative disease that affects nearly 80 million people worldwide [1]. It is associated with elevated IOP, which can lead to irreversible blindness. Relatively little is known about IOP dynamics and the physiological factors that affect it as IOP is typically monitored using tonometry. Tonometry is a common tool used by clinicians and researchers to measure IOP noninvasively. It provides a good estimate of IOP mean but not variance because data collection takes time. Readings can also be influenced by subject …

Potassium-Selective Channelrhodopsins, Elena G Govorunova, Oleg A Sineshchekov, John L Spudich

Faculty, Staff and Student Publications

Since their discovery 21 years ago, channelrhodopsins have come of age and have become indispensable tools for optogenetic control of excitable cells such as neurons and myocytes. Potential therapeutic utility of channelrhodopsins has been proven by partial vision restoration in a human patient. Previously known channelrhodopsins are either proton channels, non-selective cation channels almost equally permeable to Na+ and K+ besides protons, or anion channels. Two years ago, we discovered a group of channelrhodopsins that exhibit over an order of magnitude higher selectivity for K+ than for Na+. These proteins, known as “kalium channelrhodopsins” or KCRs, lack the canonical tetrameric …

Editorial: Assessing And Addressing Health Inequities And Disparities: The Role Of Health Informatics, Gulzar H. Shah, Anjum Khurshid, Joanne Chopak-Foss

Health Policy & Community Health: Faculty Publications

No abstract provided.

Acupuncture Treatment For Post-Stroke Depression: Intestinal Microbiota And Its Role, Hailun Jiang, Shizhe Deng, Jieying Zhang, Junjie Chen, Boxuan Li, Weiming Zhu, Menglong Zhang, Chao Zhang, Zhihong Meng

Faculty, Staff and Student Publications

Stroke-induced depression is a common complication and an important risk factor for disability. Besides psychiatric symptoms, depressed patients may also exhibit a variety of gastrointestinal symptoms, and even take gastrointestinal symptoms as the primary reason for medical treatment. It is well documented that stress may disrupt the balance of the gut microbiome in patients suffering from post-stroke depression (PSD), and that disruption of the gut microbiome is closely related to the severity of the condition in depressed patients. Therefore, maintaining the balance of intestinal microbiota can be the focus of research on the mechanism of acupuncture in the treatment of …

Conjugation's Toolkit: The Roles Of Nonstructural Proteins In Bacterial Sex, Matthew B Cooke, Christophe Herman

Faculty, Staff and Students Publications

Bacterial conjugation, a form of horizontal gene transfer, relies on a type 4 secretion system (T4SS) and a set of nonstructural genes that are closely linked. These nonstructural genes aid in the mobile lifestyle of conjugative elements but are not part of the T4SS apparatus for conjugative transfer, such as the membrane pore and relaxosome, or the plasmid maintenance and replication machineries. While these nonstructural genes are not essential for conjugation, they assist in core conjugative functions and mitigate the cellular burden on the host. This review compiles and categorizes known functions of nonstructural genes by the stage of conjugation …

High Health Care Cost In The U.S. And Its Impact On An African American Church Community In East Orange, New Jersey, William Charles

Doctoral Dissertations and Projects

Access to affordable quality health care has been a long debating issue in the United States. Many scholarly research articles indicated that the cost of insurance premiums, deductibles, co-payments, and prescription drugs continue to rise every year. Despite various efforts to reduce the cost of health care in the United States, the trend remains the same. Au contraire, more Americans are unable to afford the cost of prescription drugs. More Americans postpone their medical treatments. More Americans find it difficult to financially address their health care needs. It can be inferred that high health cost has a significant impact in …

The Impact Of Juvenile Drug Court On Recidivism, Mallory Noelle Watkins

Doctoral Dissertations and Projects

The number of youths entering the juvenile justice system is a viable concern throughout the United States. A gap in the existing literature surrounding juvenile justice is recidivism rates among young offenders who are incarcerated or sanctioned to a community-based level of supervision after committing a transgression of a law. Federal juvenile justice practitioners, such as the Office of Juvenile Justice and Delinquency Prevention (OJJDP), the National Council of Juvenile and Family Court Judges (NCJFCJ), the United States Department of Justice (DOJ), and others, in addition to individual states and localities throughout the United States, have taken various approaches to …

Determining The Impact Of Nurse Residency Programs To Increase Retention Rate Among New Nurse Graduates: An Integrative Review, Guiselly Astrid Erazo-Romero

Doctoral Dissertations and Projects

New nurse graduates account for the largest group entering the profession, but they also have the lowest retention rates among all nursing staff. Due to the nursing shortage, newly licensed nurses undergo a shorter orientation to meet the complex needs of healthcare. Newly licensed nurses often report feelings of unpreparedness, lack of competence, and lack of confidence as they are expected to care for patients with complex needs. In response to the concern over low retention rates and the preparation-practice gap among new graduate nurses, Nurse Residency Programs (NRPs) were implemented. This program further eases the transition from the student …

Teamstepps And Organizational Culture, Amelia Arca Quinto

Doctoral Dissertations and Projects

Patient safety issues remain despite several strategies developed for their deterrence. While many safety initiatives bring about improvement, they are repeatedly unsustainable and short-lived. The index hospital’s goal was to build an organizational culture within a groundwork that improves teamwork and continuing healthcare team engagement. Teamwork influences the efficiency of patient care, patient safety, and clinical outcomes, as it has been identified as an approach for enhancing collaboration, decreasing medical errors, and building a culture of safety in healthcare. The facility implemented Team Strategies and Tools to Enhance Performance and Patient Safety (TeamSTEPPS), an evidence-based framework which was used for …

The People’S Social Epi Project: Pdx With Ryan Petteway, Ryan J. Petteway

PDXPLORES Podcast

In this episode of PDXPLORES, Ryan J. Petteway, Associate Professor at the Oregon Health & Science University-Portland State University School of Public Health, discusses the research behind The People's Social Epi Project: PDX. Providing a counternarrative to the settler-colonial and racial capitalist practices of traditional epidemiological research on health inequities, Petteway's PSEP: PDX initiative is a portfolio of three projects centering around youth-led participatory research, music, and poetry. PSEP: PDX seeks to "center the margins'' to advance health and epistemic justice.

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Cross-Modal Functional Connectivity Supports Speech Understanding In Cochlear Implant Users, Amanda M Fullerton, Deborah A Vickers, Robert Luke, Addison N Billing, David Mcalpine, Heivet Hernandez-Perez, Jonathan E Peelle, Jessica J M Monaghan, Catherine M Mcmahon

2020-Current year OA Pubs

Sensory deprivation can lead to cross-modal cortical changes, whereby sensory brain regions deprived of input may be recruited to perform atypical function. Enhanced cross-modal responses to visual stimuli observed in auditory cortex of postlingually deaf cochlear implant (CI) users are hypothesized to reflect increased activation of cortical language regions, but it is unclear if this cross-modal activity is "adaptive" or "mal-adaptive" for speech understanding. To determine if increased activation of language regions is correlated with better speech understanding in CI users, we assessed task-related activation and functional connectivity of auditory and visual cortices to auditory and visual speech and non-speech …

Vaccine Effectiveness Against Influenza A(H3n2)-Associated Hospitalized Illness: United States, 2022, Mark W Tenforde, Jennie H Kwon, Et Al.

2020-Current year OA Pubs

BACKGROUND: The COVID-19 pandemic was associated with historically low influenza circulation during the 2020-2021 season, followed by an increase in influenza circulation during the 2021-2022 US season. The 2a.2 subgroup of the influenza A(H3N2) 3C.2a1b subclade that predominated was antigenically different from the vaccine strain.

METHODS: To understand the effectiveness of the 2021-2022 vaccine against hospitalized influenza illness, a multistate sentinel surveillance network enrolled adults aged ≥18 years hospitalized with acute respiratory illness and tested for influenza by a molecular assay. Using the test-negative design, vaccine effectiveness (VE) was measured by comparing the odds of current-season influenza vaccination in influenza-positive …

Exercise Training To Improve Brain Health In Older People Living With Hiv: Study Protocol For A Randomized Controlled Trial, Sarah Cooley, Brittany M Nelson, Alexander Rosenow, Elizabeth Westerhaus, W Todd Cade, Dominic N Reeds, Florin Vaida, Kevin E Yarasheski, Robert H Paul, Beau M Ances

2020-Current year OA Pubs

BACKGROUND: With the advent of antiretrovirals, people living with HIV are living near-normal lifespans. However, people living with HIV are at greater risk of experiencing cognitive impairment and reduced brain integrity despite well-controlled viremia. A robust literature supports exercise interventions as a method of improving cognition and structural brain integrity in older individuals without HIV. The effects of exercise on cardiometabolic, neurocognitive, and neural structures in middle-aged to older people living with HIV are less well known, with few prospective studies examining these measures.

OBJECTIVE: This prospective randomized clinical trial will examine the effects of a 6-month exercise training intervention …

Allosteric Effects Of E. Coli Ssb And Recr Proteins On Reco Protein Binding To Dna, Min Kyung Shinn, Sumit K Chaturvedi, Alexander G Kozlov, Timothy M Lohman

2020-Current year OA Pubs

Escherichia coli single stranded (ss) DNA binding protein (SSB) plays essential roles in DNA maintenance. It binds ssDNA with high affinity through its N-terminal DNA binding core and recruits at least 17 different SSB interacting proteins (SIPs) that are involved in DNA replication, recombination, and repair via its nine amino acid acidic tip (SSB-Ct). E. coli RecO, a SIP, is an essential recombination mediator protein in the RecF pathway of DNA repair that binds ssDNA and forms a complex with E. coli RecR protein. Here, we report ssDNA binding studies of RecO and the effects of a 15 amino acid …

Prediction Of Coronary Artery Disease Using Urinary Proteomics, Dongmei Wei, Jesus D. Melgarejo, Lucas Van Aelst, Thomas Vanassche, Peter Verhamme, Stefan Janssens, Karlheinz Peter, Zhen-Yu Zhang

School of Medicine Publications

Aims: Coronary artery disease (CAD) is multifactorial, caused by complex pathophysiology, and contributes to a high burden of mortality worldwide. Urinary proteomic analyses may help to identify predictive biomarkers and provide insights into the pathogenesis of CAD.

Methods and results: Urinary proteome was analysed in 965 participants using capillary electrophoresis coupled with mass spectrometry. A proteomic classifier was developed in a discovery cohort with 36 individuals with CAD and 36 matched controls using the support vector machine. The classifier was tested in a validation cohort with 115 individuals who progressed to CAD and 778 controls and compared with two previously …

Increased Prevalence Of Suicide In The Adolescent Population Within The Lgbtq+ Community Compared To Adolescents In The Heterosexual Population: An Integrative Review, Natasha Denese Causey

Doctoral Dissertations and Projects

According to research, young Americans who identify as lesbian, gay, bisexual, transgender, queer, and more (LGBTQ+) are currently experiencing a mental health crisis. Suicide ideations reportedly begin sooner in LGBQ+ adolescents, 13 to 18 years of age. Suicide ideation is defined by the National Alliance of Mental Illness (NAMI), as having serious thoughts about suicide with a plan. This involves LGBTQ+ adolescents shifting from suicidal ideation to planning more quickly than their heterosexual (straight) peers. Adolescents who identify as LGBTQ+ showed a higher lifetime risk of suicide thoughts than their heterosexual peers (26.1 percent to 13 percent, respectively). Additionally, 5.4 …

Preceptor Training: Evaluation Of An On-Line Educational Module To Improve Preceptor Feedback, Karina Gonzalez, Matthew J. Drescher, Elizabeth R. Neil, Lindsey E. Eberman

Internet Journal of Allied Health Sciences and Practice

Purpose: Effective feedback delivery is critical to behavior modification and skill improvement in novice learners and athletic training programs often use annual training to teach preceptors to develop feedback skills. The purpose of this research project was to evaluate the effectiveness of an on-line asynchronous educational module for improving feedback delivery. Method: We used a single cohort, repeated measures design (pre, post, post-post) and an eAuthoring tool (Softchalk^©) and online assessments (Qualtrics® Provo, UT) to evaluate the educational module. Preceptors (n=351) from 17 post-baccalaureate programs began the study; 48 completed all 3 assessments. We delivered a content validated …

Prevalence Of Self-Reported Adhd Symptoms Among Collegiate Athletes, S. Andrew Cage, Lynzi Warner, Alan Cook, Rebecca Swindall, Carly Wadle, Brandon J. Warner

Internet Journal of Allied Health Sciences and Practice

Purpose: Attention deficit hyperactivity disorder (ADHD) is a neuropsychiatric disorder that impacts an individual’s ability to maintain attention span and focus their concentration at a normal level. While ADHD is one of the most well studied behavioral disorders, multiple researchers have stated the need for further research on the effects of ADHD on participation in sports. Therefore, the purpose of this study was to describe the prevalence of self-reported ADHD symptoms among collegiate student-athletes at an NCAA institution. A secondary purpose was to determine differences in self-reported ADHD symptoms based on demographic information and history of concussion. Methods: Subjects …

Inclusion Of Sexual Orientation And Gender Identity (Sogi) Cultural Competence In Higher Education Healthcare Programs: A Scoping Review, Kristin Willey, Jennifer K. Fortuna, Jessica Guerra, Amanda Gross, Samantha Turner, Tara Grant, Betsy Williams

Internet Journal of Allied Health Sciences and Practice

Purpose: Lack of sexual orientation and gender identity (SOGI) cultural competence in healthcare providers contributes to poor health outcomes in individuals who are lesbian, gay, bisexual, transgender, queer or questioning, intersex, asexual, and more (LGBTQIA+). However, SOGI is often overlooked in healthcare education. Existing research shows educational programs in the nursing, medical, and pharmacy professions are incorporating cultural competence training into the curricula. Few studies have explored how SOGI cultural competence is incorporated into occupational therapy (OT), physical therapy (PT), and speech-language pathology (SLP) curricula. Clear guidelines for training on SOGI cultural competence are lacking in these professions. It is …

Preceptors’ Experiences Training Public Health Interns In Remote Workplace Settings, Olivia S. Anderson, Ella August

Internet Journal of Allied Health Sciences and Practice

Background: The discourse around COVID-19 related challenges to classroom instruction has been highly visible, but little has been published on learning experiences outside the classroom that prepare students to work in the “real world.” Method: We interviewed five Master of Public Health internship preceptors about their experience in 2020 with student interns to formulate pertinent survey questions to distribute to a larger sample. Our questionnaire included closed- and open-ended questions regarding student attributes and skills that shaped the success of the internship. Data were collected from internships sites of one midwestern university. Results: Nearly all 26 preceptors that responded to …

Does Acute Fatigue Affect Lumbar Proprioception And Balance?, Ozde Depreli, Ender Angin

Internet Journal of Allied Health Sciences and Practice

Purpose: To investigate the acute effects of trunk extensor, abdominal, and calf muscle fatigue on lumbar proprioception and balance. Method: Seventy healthy individuals, aged between 18-25, without pain in the low back and lower extremities, with right dominant lower extremities, were included in the study. Dynamic balance with the Y-balance Test, lumbar proprioception sense with the application of the IOS software, and fatigue level with the 6-20 Borg Scale were evaluated before and immediately after the back extensor, abdominal, and calf muscle fatigue protocols were applied to the individuals at 72-hour intervals. Results: After all fatigue protocols, proprioception sense at …

The Abrupt Educational Switch: Impact Of Covid-19 And Lessons Learned For Health Science Faculty And Students, Kathleen Klein, Marylou Galantino, Tara Crowell, Renne Cavazza

Internet Journal of Allied Health Sciences and Practice

Purpose: The abrupt educational transition from in-class to virtual instruction during spring of 2020 found many faculty and students in uncharted territory. The purpose of this study was to survey both faculty and students in the School of Health Sciences to better understand the switch from face-to-face instruction to remote learning. Methods: Both qualitative and quantitative data was collected regarding this experience. The first task was to identify faculty’s responses and actions taken, perception of alternative teaching experience and impact on student learning, determine needs to prepare and address new course design and delivery, and explore perception of changes to …

Preparedness Of Doctor Of Athletic Training (Dat) Credentialed Athletic Trainers To Take Roles In Academia, Andrew J. Schweitzer, Matthew J. Rivera, Cailee E. Welch Bacon, Lindsey E. Eberman

Internet Journal of Allied Health Sciences and Practice

ABSTRACT

Purpose: The athletic training education landscape is shifting, which has opened new avenues for postprofessional education. Previous literature has investigated stakeholder perceptions and attitudes towards hiring a DAT degree holder in academia. Yet, there is no research investigating the experiences of DAT degree holders in academic roles. The purpose of this study was to explore the preparation and experiences of DAT degree holders related to their roles in academia. Methods: We used a consensual qualitative research design to explore the lived experiences of DAT degree holders in academia through semi-structured web-based interviews. Twelve DAT degree holders with at least …

Exploring The Transtheoretical Model Of Change, Barriers, And Motives To Telemedicine Adoption In Athletic Training, Gabrielle A. Griffin, Kenneth E. Games, David F. Gallegos, Samantha E. Scarneo-Miller, Amanda L. Kottak, Zachary K. Winkelmann

Internet Journal of Allied Health Sciences and Practice

Purpose: The delivery of healthcare services by athletic trainers (ATs) has evolved over the past two years as a result of the COVID-19 pandemic. Previous research has identified an integration of telemedicine as a necessary, urgent replacement for face-to-face patient encounters in athletic training; however, there is a lack of data to support the habitual and intentional change to one’s clinical practice that includes telemedicine. Therefore, the purpose of this study was to identify and analyze ATs respective stages of change related to telemedicine adoption, as well as explore motivating factors and barriers related to the adoption of telemedicine. Methods: …

Conservative Treatment Of A Symptomatic Os Vesalianum Pedis In A Professional Soccer Player: A Case Report, Mario F. Cruz, Lori A. Bolgla, Ryan Alexander, Peter J. Symbas, Jeremy Royal, Jordan Serrano-Dennis

Internet Journal of Allied Health Sciences and Practice

Objectives: Symptomatic os vesalianum pedis (OVP), a small accessory bone located adjacent to the base of the 5^th metatarsal of the foot, rarely affects individuals who participate in sports. The condition typically is treated with surgical intervention. This case report documents the examination, evaluation, and treatment approach for a professional soccer player treated conservatively with a symptomatic OVP. Methods: A nineteen-year-old professional soccer player presented with pain to the base of the left 5^th metatarsal. Physical examination and imaging resulted in a diagnosis of a symptomatic OVP. The player underwent a 5-stage criterion-based rehabilitation program. Results: The player …

Comparing Two Methods Of Clinical Supervision, Heather K. Anderson, Sandra Hayes, Julie P. Smith, Stephanie Currie

Internet Journal of Allied Health Sciences and Practice

Purpose: The purpose of this study was to compare two methods of clinical supervision, remote and in-person, in group speech-language therapy in a university-based outpatient clinic, in terms of student clinical behaviors that occur with each method. Clinical supervision is an essential part of graduate education programs in speech-language pathology and clinical supervisors in university-based speech and hearing clinics often employ remote supervision. The professional literature concerning the impact of remote supervision on student clinical behaviors is limited. The studies found advantages and disadvantages to in-person and remote supervision and no real preference for one method was noted by participants. …

The Current Youth Sport Culture And Its Impact On Sport Participation Experiences Of Low Socioeconomic Status Families, Mayrena I. Hernandez, Elena C. Miller, Laura A. Prieto, Luis Columna, Kevin M. Biese, David R. Bell

Internet Journal of Allied Health Sciences and Practice

Purpose: The rise of sport specialization can jeopardize the opportunities for families of low socioeconomic status (SES) to participate in organized sport. However, obtaining an athletic scholarship may be a motivating factor for low SES youth to sport specialize. Yet, the experiences of low SES athletes in sport participation are not well known. Method: We used an Interpretative phenomenological analysis (IPA) to explore experiences of low SES families with children participating in sport and how this may be impacted by the current youth sport culture that promotes sport specialization. We engaged in semi-structured interviews with 12 low SES parents. Results: …