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Safety And Efficacy Of The Novel Surgical Dye From Blue Butterfly Pea Flower: An Ex Vivo And In Vitro Study, Rossukon Khotcharrat, Wanachat Thongsuk, Pussadee Paensuwan

Chulalongkorn Medical Journal

Background: The chemical dyes were used for anterior capsule staining with caution. There is no natural dye available. The extract from traditional Thai herb may be an alternative dye. Objectives: To assess the in vitro cytotoxicity of the novel surgical dye on corneal endothelial cells and evaluate the proper concentration for staining on anterior lens capsule during cataract surgery in ex vivo. Methods: Safety of the dye in various concentrations on human corneal endothelial cell line was evaluated by resazurin technique. The efficacy of human anterior capsular staining at different concentrations of the dye and different time-points was also studied …