Medicine and Health Sciences Commons^™

The Ethics Of Ivf, Breanna Beers

Bioethics in Faith and Practice

In vitro fertilization (IVF) has promised hope to many couples struggling with the pain of infertility. However, as with any new medical technology, the ethical implications of this procedure must be examined, particularly in light of recent events such as the birth of the first genetically modified human beings, made possible by IVF. It is crucial to examine oppositions to IVF based on principle, as well as address concerns related to adjacent issues such as the discard of unused embryos; the selection, payment, privacy, and parental rights of donors and surrogates; the importance of genetic parenthood; new combinations of gametes; …

The Non-Coding Rnome After Splenectomy, Mihnea P Dragomir, Stefan Tudor, Keishi Okubo, Masayoshi Shimizu, Meng Chen, Dana Elena Giza, William Ruixian He, Cristina Ivan, George A Calin, Catalin Vasilescu

Faculty and Staff Publications

Splenectomy is a common surgical procedure performed in millions of people worldwide. Epidemiologic data show that splenectomy is followed by infectious (sepsis) and non-infectious complications, with unknown mechanisms. In order to explore the role of the non-coding transcripts involved in these complications, we analysed a panel of circulating microRNAs (miRNAs), which were previously reported to be deregulated in sepsis, in the plasma of splenectomized patients. MiR-223 was overexpressed immediately and late after splenectomy, while miR-146a was overexpressed immediately after splenectomy, returning latter to basal levels; and miR-16, miR-93, miR-26a and miR-26b were overexpressed only late after splenectomy, suggesting similarities with …

Advances In Gene Ontology Utilization Improve Statistical Power Of Annotation Enrichment, Eugene Waverly Hinderer Iii, Robert M. Flight, Rashmi Dubey, James N. Macleod, Hunter N. B. Moseley

Maxwell H. Gluck Equine Research Center Faculty Publications

Gene-annotation enrichment is a common method for utilizing ontology-based annotations in gene and gene-product centric knowledgebases. Effective utilization of these annotations requires inferring semantic linkages by tracing paths through edges in the ontological graph, referred to as relations. However, some relations are semantically problematic with respect to scope, necessitating their omission or modification lest erroneous term mappings occur. To address these issues, we created the Gene Ontology Categorization Suite, or GOcats—a novel tool that organizes the Gene Ontology into subgraphs representing user-defined concepts, while ensuring that all appropriate relations are congruent with respect to scoping semantics. Here, we demonstrate the …

Detection Of Genetic Thrombophilia (Fa V Leiden & Pt20210 Mutation) Using Genexpert Technology With Iqcp Implementation, Mariegrace Saquilayan

Natural Sciences and Mathematics | <br />Clinical Laboratory Sciences 2016 - 2019

Abstract

Individuals having Genetic Thrombophilia pose a higher risk of having a thrombotic event. It is crucial to determine these gene variants to prevent a possible episode of thromboembolism. With the current PCR method, it involves individual processing of DNA isolation, amplification, and detection using three (3) different instruments resulting to an increased turnaround time of 5 to 7 days and additional staff utilization. It is performed by repetitive manual sample pipetting and preparation of reagent master mixes in small vials. Results interpretations are entered manually to a worklist built initially for final verification. These processes increase the risk of …

Association Between Survivin Gene Polymorphism And Colorectal Cancer, Belal Montaser, Mohamed S. Amar, Mona Maamoun Ahmed, Waleed M. Fathy

Menoufia Medical Journal

Objective To study the possible association of genetic polymorphism of survivin gene (rs9904341) with the risk of developing colorectal cancer (CRC) in Egyptian patients and explore the mechanisms of survivin polymorphism in the development of CRC. Background Survivin gene is an inhibitor of apoptosis, plays an important role in cell cycle regulation, and may be involved in the development and progression of cancer. Structurally, the human survivin gene comprises four exons and three introns spanning 14.7 kb, which encodes a 16.5 kDa protein. Survivin gene is expressed mostly at the G2/M phase and declines rapidly in the G1 phase of …

Mitochondrial Superoxide Disrupts The Metabolic And Epigenetic Landscape Of Cd4, Cassandra M. Moshfegh, Christopher W. Collins, Venugopal Gunda, A. Vasanthakumar, J. Z. Cao, Pankaj K. Singh, L. A. Godley, Adam J. Case

Journal Articles: Eppley Institute

While the role of mitochondrial metabolism in controlling T-lymphocyte activation and function is becoming more clear, the specifics of how mitochondrial redox signaling contributes to T-lymphocyte regulation remains elusive. Here, we examined the global effects of elevated mitochondrial superoxide (O₂^-) on T-lymphocyte activation using a novel model of inducible manganese superoxide dismutase (MnSOD) knock-out. Loss of MnSOD led to specific increases in mitochondrial O₂^- with no evident changes in hydrogen peroxide (H₂O₂), peroxynitrite (ONOO^-), or copper/zinc superoxide dismutase (CuZnSOD) levels. Unexpectedly, both mitochondrial and glycolytic metabolism showed significant reductions …

Malignant Hyperthermia, Alexandra Mcguire

Nursing Student Class Projects (Formerly MSN)

Malignant Hyperthermia (MH) is a rare but life-threatening, genetic condition that can manifest when a patient is exposed to certain inhalation agents used in anesthesia as well as the depolarizing muscle relaxant succinylcholine (Rosenberg, Pollock, Schiemann, Bulger, & Stowell, 2015, p. 1). MH can develop during or after a surgical procedure and if left untreated, can cause major organ system damage and death (Seifert, Wahr, Pace, Cochrane, & Bagnola, 2014, p. 189). Creating awareness of this rare genetic condition allows for quicker interventions in reversing MH.