Medicine and Health Sciences Commons^™

Effects Of Light On Riboflavin And Ascorbic Acid In Freshly Expressed Human Milk, Jimi Francis, Darby Dickton

Health and Kinesiology Faculty Publications and Presentations

Millions of dollars each year is spent funding dairy research to better understand every aspect of milk processing, storage, handling, and shelf life. The dairy industry has shown that in animal milks vitamin C is photo-oxidized when exposed to light, which can cause a cascade of other nutrients that may be affected. Expressed human breast milk has had limited research published, mainly recommendations for storage duration secondary to bacterial growth, with scant research on nutrient quality during handling compared to the animal models. In this study, freshly expressed human milk was placed in containers of varying color/UV sensitivity and exposed …

Electrolyte And Ascorbic Acid Supplementation For Heat Exposed Broilers In Thailand, Chackrit Nuengjamnong, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

The objective of this study was to compare the effects of ascorbic acid and mixed electrolytes on growth performance, ammonia emission and serum antioxidant enzyme activity in broilers during summer period. Two hundred and ten male and female Arbor Acre broilers during grower-finisher period (22-42 days old) were allocated into 3 groups with different additives in drinking water: 1,000 ppm ascorbic acid (T1); 2,000 ppm mixed electrolytes containing only Na+, K+ and HCO3- (T2) and none (T3 serving as control group). The experiment was conducted in an open broiler house during the summer period between April and May. Results indicated …

Ascorbic Acid And Ascorbate-2-Phosphate Decrease Hif Activity And Malignant Properties Of Human Melanoma Cells, Sarah Miles, Adam P. Fischer, Sandeep J. Joshi, Richard M. Niles

Biochemistry and Microbiology

Background: Hypoxia inducible factor-1 alpha (HIF-1α) is thought to play a role in melanoma carcinogenesis. Posttranslational regulation of HIF-1α is dependent on Prolyl hydroxylase (PHD 1–3) and Factor Inhibiting HIF (FIH) hydroxylase enzymes, which require ascorbic acid as a co-factor for optimal function. Depleted intra-tumoral ascorbic acid may thus play a role in the loss of HIF-1α regulation in melanoma. These studies assess the ability of ascorbic acid to reduce HIF-1α protein and transcriptional activity in metastatic melanoma and reduce its invasive potential.

Methods: HIF-1α protein was evaluated by western blot, while transcriptional activity was measured by HIF-1 HRE-luciferase reporter …