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Full-Text Articles in Medicine and Health Sciences
Squamous Cell Carcinoma Response To Cisplatin Exposure, Khanh V. Luong
Squamous Cell Carcinoma Response To Cisplatin Exposure, Khanh V. Luong
Theses & Dissertations
Purpose: This thesis attempted to quantitatively analyze the individual cell fate choice in resistant head and neck UM-SCC38 cells exposed to cisplatin using the most current techniques available.
Methods: UM-SCC-38 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). They were treated with cisplatin and ATM/ATR inhibitors of known dosages. Using live cell imaging, one hundred cells were tracked in each experiment and their behaviors were analyzed and entered into Microsoft Excel Spreadsheet to generate cell profile graphs. HaCaT cells, non-tumorigenic keratinocyte cell line, were also analyzed using live cell imaging and their …
Integration Of Atm, Atr, And Dna-Pkcs Signaling Maintains Genome Integrity During Neurogenesis, Vanessa D. Enriquez-Rios
Integration Of Atm, Atr, And Dna-Pkcs Signaling Maintains Genome Integrity During Neurogenesis, Vanessa D. Enriquez-Rios
Theses and Dissertations (ETD)
The DNA damage response (DDR) orchestrates a network of cellular processes such as cell cycle progression, DNA repair, and apoptosis when complex DNA lesions arise to maintain genomic integrity. ATM, ATR, and DNA-PKcs (encoded by PRKDC) are related phosphatidylinositol-3-kinase like serine/threonine kinases (PIKK) that collectively regulate the DDR network. Studies have demonstrated these kinases can phosphorylate many of the same substrates, suggesting a significant potential for functional redundancy. However, deficiencies in these kinases have been linked to distinct neural degenerative and developmental disorders, underscoring their unique functions for maintain genomic integrity during nervous system development.
Here we utilized mouse genetic …