Medicine and Health Sciences Commons^™

Expanding Access To Malaria Diagnosis Through Retail Shops In Western Kenya: What Do Shop Workers Think?, Andria Rusk, Catherine Goodman, Violet Naanyu, Beatrice Koech, Andrew Obala, Wendy Prudhomme O'Meara

Collected Faculty and Staff Scholarship

Background. The common symptoms of malaria reduce the specificity of clinical diagnosis. Presumptive treatment is conventional but can lead to overdiagnosis of malaria, delay of appropriate treatment, overprescription of antimalarials, and drug resistance. Routine use of diagnostic tests can address many of these concerns. Though treatment is often procured from retailers, there is low availability of rapid diagnostic tests for malaria (MRDTs), a simple, inexpensive, and accurate diagnostic solution. We know little about the challenges to expanding access to diagnostics through these outlets. Methods. To understand the perceptions of the benefits and challenges to selling rapid diagnostic tests for malaria, …

Genotyping Of Plasmodium Falciparum By Allele-Specificamplification Of The Merozoite Surface Protein-1 Locus, W Aichanapun, S Seethamchai, T Tia, C. Putaporntip

Chulalongkorn Medical Journal

Problem/background : Allele-specific amplification is an important tool for a largescale study of geographic distribution of pathogens harboringpolymorphic genes. In this study, we have developed a simplemethod to differentiate strains of Plasmodium falciparum basedon polymorphic block 2 located at the 5’ portion of the merozoitesurface protein-1 gene (PfMsp-1). Block 2 of PfMsp-1, a strongcandidate for asexual blood-stage vaccine, contains 3 basicsequence types with MAD20 type, K1 type and RO33 type asrepresentative alleles.Objective : To develop a polymerase chain reaction (PCR) method capableof detecting PfMsp-1 genotypes.Design : Experimental study.Setting : Department of Parasitology, Faculty of Medicine, ChulalongkornUniversity.Materials and Methods : The …

Identifikasi Vektor Malaria, Solikhah Solikhah

Kesmas

Kabupaten Kulonprogo merupakan salah satu daerah endemis malaria di Daerah Istimewa Yogyakarta dan Jawa Tengah. Lonjakan jumlah kasus malaria terjadi hampir dua kali lipat sejak tahun 2010 hingga bulan Agustus 2011. Penelitian ini bertujuan untuk mengetahui jenis Plasmodium pada penyakit malaria. Penelitian eksplorasi ini menggunakan desain studi cross sectional. Diagnosis kasus malaria diperiksa menggunakan pemeriksaan darah tebal secara mikroskopis dan untuk mengetahui faktor sosiodemografi dilakukan wawancara menggunakan kuesioner. Populasi studi adalah penduduk di wilayah Kabupaten Kulonprogo yang diambil secara proporsional pada bulan Januari sampai bulan Juni 2012. Data dianalisis secara univariat dalam bentuk grafik dan tabulasi. Hasil penelitian ini adalah …

Perception Of Disease Risk And Vulnerability As A Function Of Proximity To National Park Boundaries In East Africa, Irene Bridget Feretti

Honors Theses and Capstones

Studies suggest households closest to parks and protected areas (PAs) are more likely to sustain park-related losses, but the relationship between human sickness and PAs has not been fully explored. Existing literature primarily focuses on human-wildlife conflicts (i.e. crop raiding) and the potential for zoonotic disease spillover and emergence at the human-livestock-wildlife interface at PA boundaries. Understanding local perceptions of disease risk and vulnerability is essential for assessing human health relative to conservation areas. This understanding will promote better-informed consideration of human health impacts in decision making for conservation. Data from surveys taken at 301 households around Kibale National Park …

Persepsi, Peluang Aksi, Dan Infomasi Serta Perilaku Pencegahan Malaria, Elviera Gamelia, Siwi Pramatama Mars Wijayanti

Kesmas

Di dunia, malaria masih menjadi masalah kesehatan masyarakat terutama di negara-negara tropis termasuk Indonesia. Di Jawa Tengah, kasus malaria terus memperlihatkan kecenderungan yang meningkat, salah satu daerah endemis malaria di Banyumas adalah Puskesmas II Sumpiuh. Berdasarkan survei pendahuluan masih banyak ditemukan perilaku berisiko yang dapat mengakibatkan terjadi malaria. Penelitian ini bertujuan mengetahui persepsi masyarakat, kemungkinan aksi, dan informasi tentang malaria yang berpengaruh terhadap perilaku pencegahan malaria di Puskesmas Sumpiuh II Banyumas. Penelitian observasional dengan pendekatan cross sectional ini dilakukan pada populasi kepala keluarga di Puskesmas II Sumpiuh. Jumlah sampel sebanyak 95 kepala keluarga ditarik menggunakan metode simple random sampling. Pengumpulan …

Automated Point-Of-Care Image Processing Methodology For The Diagnosis Of Malaria, Michael B. Jorgensen

Master's Theses

Malaria has profoundly influenced human history for over four thousand years and despite numerous attempts at eradication, the prevention, diagnosis, and treatment of malaria have been largely ineffective. More than five hundred million people are affected by malaria every year resulting in over one million deaths. Drug resistance development by the parasite has diminished the effectiveness of numerous treatment options due, in part, to overtreatment of negative patients based on insufficient clinical algorithms and diagnostic methods. The goal of this research was to develop an image analysis algorithm to diagnose malaria with a high degree of sensitivity and specificity in …

Faktor Lingkungan Dan Perilaku Masyarakat Tentang Malaria Di Kecamatan Kupang Timur Kabupaten Kupang, Karolus Ngambut, Oktafianus Sila

Kesmas

Penelitian ini bertujuan untuk menggambarkan faktor lingkungan dan perilaku masyarakat tentang malaria di Kecamatan Kupang Timur Kabupaten Kupang. Dengan desain studi potong lintang, populasi penelitian adalah seluruh rumah tangga di Kecamatan Kupang Timur dengan metode simple random sampling dan diperoleh 185 rumah tangga yang dijadikan sampel. Hasil penelitian menunjukkan prevalens malaria klinis adalah 108 (14,4%) pada satu tahun terakhir. Gambaran faktor lingkungan rumah penderita malaria yaitu dinding rumah terbuat dari bebak dan papan masing-masing 43,6% dan 7,9%. Selain itu, atap rumah terbuat dari alang-alang atau daun lontar (21,1%). Sementara letak rumah dekat dengan breding places nyamuk anopheles, yaitu sawah dan …

Usefulness Of Plasmodium Falciparum-Specific Rapid Diagnostic Tests For Assessment Of Parasite Clearance And Detection Of Recurrent Infections After Artemisinin-Based Combination Therapy, B. Aydin-Schmidt, M. Mubi, U. Morris, M. Petzold, B.E. Ngasala, Zul Premji, A. Bjorkman, A. Martensson

Pathology, East Africa

Background: Rapid diagnostic test (RDT) is an important tool for parasite-based malaria diagnosis. High specificity of RDTs to distinguish an active Plasmodium falciparum infection from residual antigens from a previous infection is crucial in endemic areas where residents are repeatedly exposed to malaria. The efficiency of two RDTs based on histidine-rich protein 2 (HRP2) and lactate dehydrogenase (LDH) antigens were studied and compared with two microscopy techniques (Giemsa and acridine orange-stained blood smears) and real-time polymerase chain reaction (PCR) for assessment of initial clearance and detection of recurrent P. falciparum infections after artemisinin-based combination therapy (ACT) in a moderately high …

Malaria Diagnosis And Treatment Practices Following Introduction Of Rapid Diagnostic Tests In Kibaha District, Coast Region, Tanzania, M. Mubi, D. Kakoko, B. Ngasala, Zul Premji, S. Peterson, A. Bjorkman, A. Martensson

Pathology, East Africa

Background: The success of the universal parasite-based malaria testing policy for fever patients attending primary health care (PHC) facilities in Tanzania will depend highly on health workers' perceptions and practices. The aim of this study was, therefore, to assess the present use of malaria diagnostics (rapid diagnostic tests (RDTs) and microscopy), prescription behaviour and factors affecting adherence to test results at PHC facilities in Kibaha District, Coast Region, Tanzania.

Methods: Exit interviews were conducted with fever patients at PHC facilities and information on diagnostic test performed and treatment prescribed were recorded. Interviews with prescribers to assess their understanding, perceptions and …

Temporal Trends Of Molecular Markers Associated With Artemether- Lumefantrine Tolerance/Resistance In Bagamoyo District, Tanzania, M. Malmberg, B. Ngasala, P.E. Ferreira, E. Larsson, I. Jovel, A. Hjalmarsson, M. Petzold, Zul Premji, J.P. Gil, A. Bjorkman, A. Martensson

Pathology, East Africa

Background: Development and spread of Plasmodium falciparum resistance to artemisinin-based combination therapy (ACT) constitutes a major threat to recent global malaria control achievements. Surveillance of molecular markers could act as an early warning system of ACT-resistance before clinical treatment failures are apparent. The aim of this study was to analyse temporal trends of established genotypes associated with artemether-lumefantrine tolerance/resistance before and after its deployment as first-line treatment for uncomplicated malaria in Tanzania 2006.

Methods: Single nucleotide polymorphisms in the P. falciparum multidrug resistance gene 1 (pfmdr1) N86Y, Y184F, D1246Y and P. falciparum chloroquine transporter gene (pfcrt) K76T were analysed from …

Community Knowledge, Attitude And Practice About Malaria In A Low Endemic Setting Of Shewa Robit Town, Northeastern Ethiopia, Andargie Abate, Abraham Degarege, Berhanu Erko

Journal Articles: Epidemiology

BACKGROUND: Since malaria is one of the foremost public health problems in Ethiopia, assessment of situation of the disease, and communities' knowledge and perceptions about malaria is necesary to institute appropriate preventive and control measures. Thus, the aim of this study was to assess malaria prevalence and knowledge, attitude and practice (KAP) about the disease among ShewaRobit Town community, northeastern Ethiopia.

METHODS: A community-based cross-sectional study was conducted in Shewa Robit Town from October to November 2011. A multi-stage random sampling technique was used to select the study participants. A total of 425 individuals were examined for malaria using thin …

The Effect Of Drug Resistance On Plasmodium Falciparum Transmission And Gametocyte Development, Samantha Olivia Aylor

USF Tampa Graduate Theses and Dissertations

In order to reduce malaria prevalence worldwide, a better understanding of parasite transmission and the effect of drug resistance is needed. The effect of drug resistance on malaria transmission has been examined for some drugs, but not for mitochondrial inhibitors such as atovaquone and the current basis of malaria therapy, artemisinin. Therefore, the goal of this study was to produce gametocytes, the life cycle stage that transmits from mosquito to human, in several different drug resistant patient isolates as well as to determine the effect of drug resistance on gametocyte development and transmission. Previous studies have shown that the mutation …

Genetic Diversity Of Plasmodium Vivax Clinical Isolates From Southern Pakistan Using Pvcsp And Pvmsp1 Genetic Markers, Afsheen Raza, Najia K. Ghanchi, Ali M. Thaver, Sana Jafri, Mohammad A. Beg

Department of Pathology and Laboratory Medicine

Background:
Plasmodium vivax is the prevalent malarial species accounting for 70% of malaria burden in Pakistan; however, there is no baseline data on the circulating genotypes. Studies have shown that polymorphic loci of gene encoding antigens pvcsp and pvmsp1 can be used reliably for conducting molecular epidemiological studies. Therefore, this study aimed to bridge the existing knowledge gap on population structure on P. vivax from Pakistan using these two polymorphic genes.
Methods:
During the period January 2008 to May 2009, a total of 250 blood samples were collected from patients tested slide positive for P. vivax, at the Aga Khan …