Medicine and Health Sciences Commons^™

Cooperation Between Somatic Ikaros And Notch1 Mutations At The Inception Of T-All, Yovina Sontani, Gavin Chapman, Peter Papathanasiou, Sally Dunwoodie, Christopher C. Goodnow, Gerard F. Hoyne

Health Sciences Papers and Journal Articles

To understand the interactions between Notch1 and Ikaros in the evolution of T cell acute lymphoblastic leukemia (T-ALL), we traced the evolution of T-ALL in mice with an inherited Ikaros mutation, Ikzf1^Plstc which inactivates DNA binding. DNA-binding Ikaros repressed Notch1 response in transfected cell lines and in CD4+8+ (DP) thymocytes from young pre-leukemic Ikzf1^Plstc heterozygous mice. In DP thymocytes, a 50-1000 fold escalation in mRNA for Notch1 target genes Hes1 and Dtx1 preceded thymic lymphoma or leukemia and was closely correlated with the first detectable differentiation abnormalities loss of heterozygosity (LOH) eliminating wild-type Ikzf1, and multiple missense …

Visualizing The Role Of Cbl-B In Control Of Islet-Reactive Cd4 T Cells And Susceptibility To Type 1 Diabetes, Gerard F. Hoyne, Eleanor Flening, Mehmet Yabas, Charis Teh, John A. Altin, Katrina Randall, Christine B F Thien, Wallace Y. Langdon, Christopher C. Goodnow

Health Sciences Papers and Journal Articles

The E3 ubiquitin ligase Cbl-b regulates T cell activation thresholds and has been associated with protecting against Type 1 diabetes, but its in vivo role in the process of self-tolerance has not been examined at the level of potentially auto-aggressive CD4+ T cells. Here we visualize the consequences of Cbl-b deficiency on self-tolerance to lysozyme antigen expressed in transgenic mice under control of the insulin promoter (insHEL). By tracing the fate of pancreatic islet-reactive CD4+ T cells in pre-diabetic 3A9-TCR x insHEL double-transgenic mice, we find that Cbl-b deficiency contrasts with AIRE or IL-2 deficiency because it does not affect …

Lifestyle And Demographic Correlates Of Poor Mental Health In Early Adolescence, Monique Robinson, Garth Kendall, Peter Jacoby, Beth P. Hands, Lawrence Beilin, Sven Silburn, Steve Zubrick, Wendy Oddy

Health Sciences Papers and Journal Articles

Aim: To determine the constellation of lifestyle and demographic factors that are associated with poor mental health in an adolescent population.

Methods: The Raine Study 14-year follow-up involved primary care givers and their adolescent children (n = 1860). The Child Behaviour Checklist (CBCL) was used to assess adolescent mental health. We examined diet, socio-demographic data, family functioning, physical activity, screen use and risk-taking behaviours with mental health outcomes using linear regression.

Results: Adolescents with higher intakes of meat and meat alternatives and ‘extras’ foods had poorer mental health status. Adverse socioeconomic conditions, higher hours of screen use and ever partaking …

Mechanisms That Regulate Peripheral Immune Responses To Control Organ Specific Autoimmunity, Gerard F. Hoyne

Health Sciences Papers and Journal Articles

The immune system must balance the need to maintain a diverse repertoire of lymphocytes to be able to fight infection with the need to maintain tolerance to self proteins. The immune system places strict regulation over the ability of T cells to produce the major T cell growth factor interleukin 2 as this cytokine can influence a variety of immune outcomes. T cells require the delivery of two signals one through the antigen receptor and a second through the costimulatory receptor CD28. The immune system uses a variety of E3 ubiquitin ligases to target signaling proteins that function downstream of …

The Associations Between Physical Activity, Screen Time And Weight From 6 To 14 Years: The Raine Study, Beth P. Hands, Paola Chivers, Helen Parker, Garth Kendall, Lawrence Beilin, Dawne Larkin

Health Sciences Papers and Journal Articles

To examine the strength and direction of the relationship between physical activity level, screen use and BMI in a cohort at ages 6, 8, 10 and 14 yrs as part of a prospective longitudinal cohort study. The sample comprised 1403 males and females who participated in the follow-up survey at 14 yrs of age between 2003 and 2005. Exploratory structural equation modelling was used to examine the interrelationships between physical activity level, BMI and screen time at 6, 8, 10 and 14 yrs. Predictors of BMI at 6, 8, 10 and 14 yrs explained 1.3, 76.1, 80.1 and 73.1 percent …

Differential Requirement For The Cd45 Splicing Regulator Hnrnpll For Accumulation Of Nkt And Conventional T Cells, Mehmet Yabas, Dale I. Godfrey, Christopher C. Goodnow, Gerard F. Hoyne

Health Sciences Papers and Journal Articles

Natural killer T (NKT) cells represent an important regulatory T cell subset that develops in the thymus and contains immature (NK1.1^lo) and mature (NK1.1^hi) cell subsets. Here we show in mice that an inherited mutation in heterogeneous ribonucleoprotein L-like protein (hnRNPLL^thunder), that shortens the survival of conventional T cells, has no discernible effect on NKT cell development, homeostasis or effector function. Thus, Hnrpll deficiency effectively increases the NKTT cell ratio in the periphery. However, Hnrpll mutation disrupts CD45RA, RB and RC exon silencing of the Ptprc mRNA in both NKT and conventional T cells, …

Extra Food Intake And Depressive Symptoms In Adolescents: Is There A Relationship?, Elizabeth Bradshaw

Theses

This pilot study explored the potential role of food on young people’s mental health. Specifically, the aim was to determine if a relationship exists between extra food intake and depressive symptoms in adolescents’ age 12-17 years. It has been documented that mental health problems in young people result from a complex interaction of risk and protective factors. Eating behaviours and food intake has been investigated in early adolescence (age 10-14), however the specific contribution of extra food on depressive symptoms in mid adolescence has not been determined.

Extra food intake and depressive symptoms were collected from girls and boys in …