Medicine and Health Sciences Commons^™

Blood Haematological-Cholesterol Profile And Antibody Titer Response Of Broilers With Added Probiotic Containing Both Bacteria And Yeast Or An Antibiotic In Drinking Water, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

An experiment was conducted to investigate the haematological and cholesterol profiles and antibody titer responses to both Newcastle disease (ND) and Infectious bronchitis (IB) vaccines in broiler chickens after drinking water supplemented with either a commercial probiotic (an Active Elements®; AE) containing Lactobacillus plantarum and Saccharomyces cerevisiae, an antibiotic Chlortetracycline (CTC), or the probiotic plus the antibiotic. Four hundred one-day-old male Cobb broiler chicks were randomly allocated to 4 treatment groups designated as follow: a non-treated control (T1), a 0.05% CTC (T2), a 1:500 AE (T3), and a combination of 0.05% CTC and 1:500 AE (T4), respectively. The chicks were …

Single Daily Dosing Ceftriaxone And Metronidazole Vs Standard Triple Antibiotic Regimen For Perforated Appendicitis In Children: A Prospective Randomized Trial., Shawn D. St Peter, Kuojen Tsao, Troy L. Spilde, G W. Holcomb Iii, Susan W. Sharp, J Patrick Murphy, Charles L. Snyder, Ronald J. Sharp, Walter S. Andrews, Daniel J. Ostlie

Manuscripts, Articles, Book Chapters and Other Papers

INTRODUCTION: Appendicitis is the most common emergency condition in children. Historically, a 3-drug regimen consisting of ampicillin, gentamicin, and clindamycin (AGC) has been used postoperatively for perforated appendicitis. A retrospective review at our institution has found single day dosing of ceftriaxone and metronidazole (CM) to be a more simple and cost-effective antibiotic strategy. Therefore, we performed a prospective, randomized trial to compare efficacy and cost-effectiveness of these 2 regimens.

METHODS: After internal review board approval (IRB no. 04 12-149), children found to have perforated appendicitis at appendectomy were randomized to either once daily dosing of CM (2 total doses per …

Effects Of The Addition Of Probiotic Containing Both Bacteria And Yeast Or An Antibiotic On Performance Parameters, Mortality Rate And Antibiotic Residue In Broilers, Boonrit Thongsong, Sarinee Kalandakanond-Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

The experiment was conducted to investigate the effects of supplementing a commercially available probiotic (an Active Elements®; AE) containing Lactobacillus plantarum and Saccharomyces cerevisiae, an antibiotic; Chlortetracycline (CTC), and the probiotic plus antibiotic, via the drinking water on broiler production performance, mortality rate and the antibiotic residue in meat. Four hundred one-day-old male Cobb broiler chicks were randomly allocated to 4 treatments; a non-treated control, a 0.05% CTC, a 1:500 AE, and a combination of CTC and AE. Chicks were fed with a commercial broiler diet ad libitum. They were reared under an environmentally controlled house for 6 weeks. The …

Monitoring Lys-TrnaLys Phosphatidylglycerol Transferase Activity, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

In some bacteria Lys-tRNA^Lys is used both in translation and for the specific addition of Lys to phosphatidylglycerol in the cytoplasmic membrane. This reaction is catalyzed by the membrane protein MprF, and the lysyl-phosphatidylglycerol formed contributes to the resistance of these bacteria to various cationic antibacterial molecules. Obtaining proteins and reconstituting an in vitro system mimicking membrane conditions is a major challenge to studying the function of membrane proteins, especially when labile substrates such as Lys-tRNA^Lys are required. Here we report methods to obtain a stable enriched membrane fraction containing MprF, and the techniques necessary to quantitatively monitor …

In Vitro Analysis Of Tobramycin-Treated Pseudomonas Aeruginosa Biofilms On Cystic Fibrosis-Derived Airway Epithelial Cells, Gregory G. Anderson, Sophie Moreau-Marquis, Bruce A. Stanton, George A. O'Toole

Dartmouth Scholarship

P. aeruginosa forms biofilms in the lungs of individuals with cystic fibrosis (CF); however, there have been no effective model systems for studying biofilm formation in the CF lung. We have developed a tissue culture system for growth of P. aeruginosa biofilms on CF-derived human airway cells that promotes the formation of highly antibiotic-resistant microcolonies, which produce an extracellular polysaccharide matrix and require the known abiotic biofilm formation genes flgK and pilB. Treatment of P. aeruginosa biofilms with tobramycin reduced the virulence of the biofilms both by reducing bacterial numbers and by altering virulence gene expression. We performed microarray analysis …

In Vitro Activity Of Tigecycline Against Acinetobacter Baumannii Strains Isolated From Nosocomial Infections, Esragül Akinci, İpek Mumcuoğlu, Pinar Öngürü, F. Nurhayat Bayazit, Sevi̇nç Ersoy Şen, Ayşe Erbay, Neri̇man Balaban, Hürrem Bodur

Turkish Journal of Medical Sciences

Aims: Acinetobacter species usually affect hospitalized patients and are involved in many infections associated with a high mortality rate. Management of infections caused by these strains is very difficult, as the strains often display multiple drug resistance, including carbapenem resistance. Tigecycline is the most recently developed semi-synthetic broad spectrum glycylcycline antibiotic. The present study was conducted to evaluate the in vitro susceptibility of Acinetobacter baumannii strains isolated from the hospitalized patients to tigecycline. Materials and Methods: Acinetobacter isolates were collected from hospitalized patients with documented nosocomial infections between June 2005 and October 2006. Only one isolate per patient was accepted. …