Medicine and Health Sciences Commons^™

The Development Of A Pcr Assay For The Detection And Identification Of Candida In Oral Samples, Oranart Matangkasombut, Jarumon Siraprapa, Issara Wongpraparatana

Chulalongkorn University Dental Journal

Objective To 0.07develop a PCR-based method for the detection and identification of Candida albicans and C. glabrata in oral samples, with a comparison of sample preparations by DNA extraction and boiling. Materials and methods Oral rinse samples were collected from 10 healthy volunteers. A portion of the samples was used for cultivation and species identification by growth on Chromogenic Candida agar, chlamydospore formation and biochemical assays. The second portion was subjected to DNA extraction, while the third portion was washed and boiled for 10 minutes. The samples were than tested by PCR using fungal specific primers (ITS) and species specific …