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Involvement Of Akt In Er-To-Golgi Transport Of Scap/Srebp: A Link Between A Key Cell Proliferative Pathway And Membrane Synthesis, X Du, Ika Kristiana, Jenny Wong, A Brown
Involvement Of Akt In Er-To-Golgi Transport Of Scap/Srebp: A Link Between A Key Cell Proliferative Pathway And Membrane Synthesis, X Du, Ika Kristiana, Jenny Wong, A Brown
Illawarra Health and Medical Research Institute
Akt is a critical regulator of cell growth, proliferation, and survival that is activated by phosphatidylinositol 3-kinase (PI3K). We investigated the effect of PI3K inhibition on activation of sterol regulatory element binding protein-2 (SREBP-2), a master regulator of cholesterol homeostasis. SREBP-2 processing increased in response to various cholesterol depletion approaches (including statin treatment) and this increase was blunted by treatment with a potent and specific inhibitor of PI3K, LY294002, or when a plasmid encoding a dominant-negative form of Akt (DN-Akt) was expressed. LY294002 also suppressed SREBP-2 processing induced by insulin-like growth factor-1. Furthermore, LY294002 treatment down-regulated SREBP-2 or -1c gene …