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Articles 1 - 4 of 4
Full-Text Articles in Medicine and Health Sciences
Ryanodine Receptor Adaptation, Michael Fill, A. Zahradníková, Carlos A. Villalba-Galea, I. Zahradník, A. L. Escobar, S. Györke
Ryanodine Receptor Adaptation, Michael Fill, A. Zahradníková, Carlos A. Villalba-Galea, I. Zahradník, A. L. Escobar, S. Györke
School of Pharmacy Faculty Articles
In the heart, depolarization during the action potential activates voltage-dependent Ca2+ channels that mediate a small, localized Ca2+ influx (ICa). This small Ca2+ signal activates specialized Ca2+ release channels, the ryanodine receptors (RyRs), in the sarcoplasmic reticulum (SR). This process is called Ca2+-induced Ca2+ release (CICR). Intuitively, the CICR process should be self-regenerating because the Ca2+ released from the SR should feedback and activate further SR Ca2+ release. However, the CICR process is precisely controlled in the heart and, consequently, some sort of negative control mechanism(s) must exist to …
Single-Channel Properties In Endoplasmic Reticulum Membrane Of Recombinant Type 3 Inositol Trisphosphate Receptor., D O Mak, S Mcbride, V Raghuram, Y Yue, Suresh K. Joseph, J K Foskett
Single-Channel Properties In Endoplasmic Reticulum Membrane Of Recombinant Type 3 Inositol Trisphosphate Receptor., D O Mak, S Mcbride, V Raghuram, Y Yue, Suresh K. Joseph, J K Foskett
Department of Pathology, Anatomy, and Cell Biology Faculty Papers
The inositol 1,4,5-trisphosphate receptor (InsP(3)R) is an intracellular Ca(2+)-release channel localized in endoplasmic reticulum (ER) with a central role in complex Ca(2+) signaling in most cell types. A family of InsP(3)Rs encoded by several genes has been identified with different primary sequences, subcellular locations, variable ratios of expression, and heteromultimer formation. This diversity suggests that cells require distinct InsP(3)Rs, but the functional correlates of this diversity are largely unknown. Lacking are single-channel recordings of the recombinant type 3 receptor (InsP(3)R-3), a widely expressed isoform also implicated in plasma membrane Ca(2+) influx and apoptosis. Here, we describe functional expression and single-channel …
Cell Surface Effects Of Androgens Between 1 Pm And 100 Nm On Rat Sertoli Cells And Two Human Prostatic Cell Lines, Lncap And Pc3: Evidence For Two Membrance Receptors, Fiona Lyng, G. R. Jones, F. F.G. Rommerts
Cell Surface Effects Of Androgens Between 1 Pm And 100 Nm On Rat Sertoli Cells And Two Human Prostatic Cell Lines, Lncap And Pc3: Evidence For Two Membrance Receptors, Fiona Lyng, G. R. Jones, F. F.G. Rommerts
Articles
Androgen-induced calcium fluxes and gap junctional intercellular communication (GJIC) were studied in three different cell types. A transient (2–3 min duration) increase in intracellular calcium levels was observed within 20–30 sec of androgen addition, which was followed by a plateau phase with steroid concentrations higher than 1 nM. The kinetics of the calcium responses were similar in immature rat Sertoli cells, which contain normal nuclear receptors; the human prostatic tumor cell line, LNCaP, which contains a mutated nuclear receptor; and the human prostatic cell line, PC3, which does not contain a nuclear receptor. The human A431 tumor cell line did …
Effect Of Calcium, Magnesium, Sodium And Potassium Levels In Seminal Plasma Of Holstein Bulls On Spermatological Characters, Seyfetti̇n Gür, Eşref Demi̇rci̇
Effect Of Calcium, Magnesium, Sodium And Potassium Levels In Seminal Plasma Of Holstein Bulls On Spermatological Characters, Seyfetti̇n Gür, Eşref Demi̇rci̇
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the levels calcium, magnesium, sodium and potassium in the seminal plasma of bulls, semen volume, sperm motility, sperm concentration and the correlation between these parametres. Five Holstein bulls constituted the material of this study. Twenty ejaculates were collected from each bull and the spermatological characteristics of these ejaculates were determined. Then, seminal plasma was separated from 3ml semen from each ejaculate by centrifugation at 1500g for 20 min at 4 °C. Calcium, magnesium, sodium and potassium concentrations in seminal plasma were determined by atomic absorption spectrophotometry. The average values for calcium, magnesium, sodium and …