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Effects Of Sperm Preparation On In Vitro Fertilization In Pig, Mongkol Techakumphu, Wichai Tantasuparak
Effects Of Sperm Preparation On In Vitro Fertilization In Pig, Mongkol Techakumphu, Wichai Tantasuparak
The Thai Journal of Veterinary Medicine
The aim of these experiments was to investigate the effects of sperm preparation on the success rate of in vitro fertilization in the pig. Three factors, the time period for capacitation, the type of sperm and the sperm motility-stimulating factors were studied by investigating their effect on the cleavage of pig follicular oocytes, after IVM-IVF-IVC. A total of 1,235 immature oocytes, collected from 3 to 6 mm follicles of gilt ovaries gathered at slaughterhouse were allocated to 3 experiments. Immature oocytes were cultivated in TCM199 NaHCO3 supplemented with 10 μg/ml of FSH/LH and 1 μg/ml of Estradiol-17ẞ for 44 hrs. …
The Effect Of Oocyte Preparation On Embryo Cleavage Rate After In Vitro Fertilization In The Pig, Mongkol Techakumphu, Wanpen Srianan
The Effect Of Oocyte Preparation On Embryo Cleavage Rate After In Vitro Fertilization In The Pig, Mongkol Techakumphu, Wanpen Srianan
The Thai Journal of Veterinary Medicine
The objective of this study was to determine the optimum condition for porcine oocyte preparation for in vitro fertilization. A total of 1,130 immature oocytes collected from superficial follicles, 3-6 mm in diameter were allocated to 3 experiments. In Experiment 1, the length of time for oocyte maturation were examined (n=469). Immature oocytes were cultivated in TCM199 NaHCO3 supplemented with 10 μg/ml of FSH/LH and 1 μg/ml of Estradiol-17ẞ for 24, 40 or 48 hrs. Approximately 20% of oocytes were fixed and stained with aceto orcein to observe the maturation rate and the remainder were fertilized in vitro by freshly …