Medicine and Health Sciences Commons^™

Inactivation Of Hit Cell Ca2+ Current By A Simulated Burst Of Ca2+ Action Potentials, L. S. Satin, S. J. Tavalin, P. D. Smolen

Pharmacology and Toxicology Publications

ABSTRACT A novel voltage-clamp protocol was developed to test whether slow inactivation of Ca2+ current occurs during bursting in insulin-secreting cells. Single insulin-secreting HIT cells were patch-clamped and their Ca2+ currents were isolated pharmacologically. A computed ,3-cell burst was used as a voltage-clamp command and the net Ca2+ current elicited was determined as a cadmium difference current. Ca2+ current rapidly activated during the computed plateau and spike depolarizations and then slowly decayed. Integration of this Ca2+ current yielded an estimate of total Ca influx. To further analyze Ca2+ current inactivation during a burst, repetitive test pulses to + 10 mV …

Lipopolysaccharide-Reactive Immunoglobulin E Is Associated With Lower Mortality And Organ Failure In Traumatically Injured Patients, Joseph T. Dipiro, Robert G. Hamilton, Thomas R. Howdieshell, N. Franklin Adkinson Jr., Arlie R. Mansberger Jr.

Publications from the Office of the Dean

Antilipopolysaccharide (anti-LPS) immunoglobulin G (IgG) and IgM have been associated with protection from LPS effects in vivo. We investigated the presence of IgE and anti-LPS in 32 patients that had experienced severe traumatic injury and in 35 healthy volunteers; we also investigated whether IgE anti-LPS was associated with important clinical events. Plasma samples were collected daily from patients in the intensive care unit and on one occasion from volunteers; the samples were assayed for IgE anti-LPS. IgE anti-LPS was assayed by enzyme-linked immunosorbent assay with monoclonal anti-human IgE as the capture antibody. Detection was accomplished with biotin-labeled LPS (Escherichia coli …

Virginia Dental Journal (Vol. 71, No. 3, 1994)

Virginia Dental Journal

No abstract provided.

Monitoring Neuromuscular Blockade At The Adductor Pollicis And Orbicularis Oculi With Split Dosing Of Mivacurium Chloride, Stephen F. Palmerton

Theses and Dissertations

Twenty ASA class I and II patients between the ages of 15 and 64 years undergoing surgical procedures requiring neuromuscular blockade and general anesthesia were selected at random to participate in this study. Patients taking medication known to interfere with neuromuscular blockade were excluded. All patients were given 2 mg of midazolam IV as a premedication. In the operating room, routine monitors were connected and baseline blood pressure, pulse and respirations were recorded with subsequent recordings at 5 and 10 minutes following induction of anesthesia.

Indirect stimulation of the ulnar nerve was achieved by placing ECG electrodes 2 cm and …

Modulation Of The Humoral Immune Response By The Sympathetic Nervous System, Tracey Lynn Spriggs

Theses and Dissertations

The immune system is critical for the maintenance of homeostasis. Due to the fact that a coordinated effort between organ systems is required for internal stability, it has been postulated that the immune system interacts with the neuroendocrine system. Clinical, anatomical, and receptor studies have provided evidence for a bi-directional communication between the nervous and immune systems. The goal of the present studies was to determine the potential influence of the sympathetic nervous system (SNS) on the primary antibody forming cell (AFC) response.

The adrenergic neurotoxin, 6-hydroxydopamine (6-OHDA), has been utilized extensively by researchers to explore the possible relationship between …

Review Of The P53 Tumor Suppressor Gene And Its Role In Gliomas, James Dearing Christian

Theses and Dissertations

The following review of the p53 tumor suppressor gene will be discussed with particular attention to its role in human gliomas, as well as the various advances that have brought this molecule to the forefront of cancer research. A review of tumorigenesis focuses on the molecular mechanisms that convey neoplastic characteristics upon a normal cell. It is discussed how the coordinate advances in chromosome analysis and molecular techniques enabled the p53 gene to be categorized as a "tumor suppressor gene" and applied to various forms of cancer, including colorectal and lung carcinoma. The focus of the review will be on …

Virginia Dental Journal (Vol. 71, No. 4, 1994)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 71, No. 2, 1994)

Virginia Dental Journal

No abstract provided.

Virginia Dental Journal (Vol. 71, No. 1, 1994)

Virginia Dental Journal

No abstract provided.

Radiosurgery For Malignant Brain Tumors, Michael Jino Yun

Theses and Dissertations

Radiosurgery using the Linear Accelerator or the Gamma Knife has proven to be an effective treatment modality for malignant brain tumors. In comparison to other treatments, radiosurgery can be performed on an outpatient basis and is noninvasive (Table 5). Due to the functional properties of radiosurgical devices, they are ideal for patients who are unable to undergo surgical removal of their brain tumors. The sharp dose drop—off beyond the tumor margin allows for high dosage tumor irradiation while sparing normal brain tissue. Many procedures that involve radiosurgery use it as a ”boost” therapy in conjunction with surgical resection and whole …

A Causal Model Of Hospital Volume, Structure And Process Indicators, And Surgical Outcomes, Myra Boles

Theses and Dissertations

This research developed and tested a conceptual model to explain why higher volumes of certain surgical procedures lead to better patient outcomes. The model incorporated hospital structural characteristics and process of care indicators to explain the volume-outcome relationship. The volume-outcome relationship was further examined longitudinally to determine stability over time and to substantiate the causality implied by the conceptual model.

A sample (n=1752) of acute-care, general hospitals was selected from hospitals that performed, in 1990, at least one surgical procedure on Medicare patients of the following: reduction of hip fracture, cholecystectomy, hip replacement, carotid endarterectomy, and pacemaker insertion. For the …