Medicine and Health Sciences Commons^™

The Combined Effect Of In-Situ Tumor And Irradiation On Peritumoral Brain Vasculature, Janice Ann Zawaski

Theses and Dissertations (ETD)

In the USA, 200,000 brain tumors are diagnosed each year with glioma representing 8.4% of the 200,000. The standard treatment for glioma consists of surgical resection, when possible, followed by radiation therapy (RT) and/or chemotherapy. Radiation therapy is one of the most effective treatments of brain tumors; however, the therapeutic ratio of RT is limited by damage to the normal tissue. We hypothesize that tumor growth has an adverse effect on the peritumoral tissue through the angiogenic/inflammatory environment it creates rendering it susceptible to further damage by RT which may be prevented by using anti-angiogenic/anti-inflammatory agents. We have developed a …

Insights Into Btb-Cul3 Ubiquitin Ligases From The Structures Of Spop-Substrate Complexes, Min Zhuang

Theses and Dissertations (ETD)

Cullin-Ring ubiquitin ligases (CRLs) are E3 complexes that specifically recognize substrates through substrate adaptors. In the largest CRL subfamily, Cul3 binds a BTB domain, and a protein-interaction domain such as MATH recruits substrates for ubiquitination. Here we present biochemical and structural analyses of the MATH and BTB domain containing protein, SPOP, which regulates diverse signaling pathways. First, we identified a conserved SPOP Binding Consensus (SBC) motif in the transcriptional regulator Ci, the protein phosphatase Puc, and the chromatin component MacroH2A. The SBC motif specifically binds the MATH domain of SPOP, and is required for Puc ubiquitination in vitro and in …

A Pharmacokinetics And Pharmacodynamics (Pk/Pd) Guided Approach To Lead Optimization Of Nitrofuranylamide Anti-Tuberculosis Agents, Nageshwar Rao Budha

Theses and Dissertations (ETD)

Currently used treatment strategies for tuberculosis (TB) involve administration of multiple drug combinations for a minimum of 6-9 months. However, these prolonged regimens do not always achieve sterilization, as evidenced by post-therapy relapse in a subgroup of treated individuals. In an effort to develop novel therapeutic agents for TB a new class of chemical agents, nitrofuranylamides, is being developed at the University of Tennessee Health Science Center. We hypothesized that the application of an iterative pharmacokinetics and pharmacodynamics (PK/PD) guided approach would facilitate the optimization of nitrofuranylamide lead compounds suitable for further development.

First, we examined the biopharmaceutic properties and …

Structural And Kinetics Studies Of The Enzyme Dihydropteroate Synthase And The Implications For Antibiotic Resistance, Katherine A. Ayers

Theses and Dissertations (ETD)

The goal of this study is to develop small molecule inhibitors of DHPS for the treatment of a variety of infectious diseases. According to the World Health Organization (WHO), infectious diseases kill more than 13 million people worldwide every year making it the second leading cause of death behind cardiovascular disease. The sulfonamide class of drugs has been in use since the 1930’s to treat many infectious agents and act by targeting the enzyme dihydropteroate synthase (DHPS) of the prokaryotic and lower eukaryotic folate pathway.

DHPS is an ideal drug target because humans do not synthesize folate de novo and …

Effect Of Obesity On The Traditional And Emerging Cardiovascular Disease Risk Factors In African American Women, Queen Obiageli Henry-Okafor

Theses and Dissertations (ETD)

Background: Obesity is a growing health care concern with cardiovascular disease (CVD) implications. African American women (AAW) have the highest prevalence rate of obesity and highest CVD morbidity and mortality rate of all ethnic groups. The traditional CVD risk factors have not been sufficient to explain this disparity in disease prevalence and outcomes. Current knowledge is limited regarding the interaction between various levels of adiposity and both traditional and emerging CVD risk factors, particularly in AAW. This study sought to explore these interactions.

Methods: The study design was a cross-sectional, descriptive, correlational analysis of 48 AAW ages 18 to 45 …

Cognitive Inference And Resulting Behaviors In Response To Ambiguous Threat In The Coyote, Canis Latrans, Sarah Shawnee Dawson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

While antipredator strategies have been a focus of behavioral research for decades, scientists generally study the responses of prey toward overt, explicit threat. However, risk can also be significant when a threat is covert, such as when an ambush predator may be nearby or a secondary threat remains after a predator's departure. Little is known about the mechanism that prey use to assess risk in a predator's absence. Tests were conducted to determine the manner in which coyotes respond to these ambiguous threats. Specifically, I tested whether coyotes respond to prior anthropogenic activity that has occurred near their only food …

A Culturally-Relevant, Emergent Approach To Exploring The Needs, Strengths, And Priorities Of Tribal Communities In Virginia, Amy Prorock-Ernest

Theses and Dissertations

Little is known about the health of the indigenous peoples in Virginia. When compared to the total U.S. population, indigenous peoples nationwide disproportionately experience disparities in health status across multiple health indicators. Research shows that these disparities are largely due to the inequitable distribution of social and economic determinants present within indigenous communities. Because the indigenous peoples of Virginia are affected by inequitable social and economic conditions, there is reason to believe that health indicators may mirror that of the indigenous population at-large. Outlined in this paper is a framework for exploring the needs, strengths, and priorities of indigenous communities …

Biomedical Language Understanding And Extraction (Blue-Text): A Minimal Syntactic, Semantic Method, Parsa Mirhaji

Dissertations and Theses (Open Access)

Clinical text understanding (CTU) is of interest to health informatics because critical clinical information frequently represented as unconstrained text in electronic health records are extensively used by human experts to guide clinical practice, decision making, and to document delivery of care, but are largely unusable by information systems for queries and computations. Recent initiatives advocating for translational research call for generation of technologies that can integrate structured clinical data with unstructured data, provide a unified interface to all data, and contextualize clinical information for reuse in multidisciplinary and collaborative environment envisioned by CTSA program. This implies that technologies for the …

Advance Practice Nurses Readiness To Change Assessment Methods In Parents Of Obese Children, Carolyn Leach

UNLV Theses, Dissertations, Professional Papers, and Capstones

The worldwide epidemic of child and adolescent overweight and obesity continues to increase at disturbing rates. Unhealthy dietary practice is one of multiple causative behaviors leading to child and adolescent overweight and obesity. This behavior can contribute to short and long-term health consequences. Parents are modulators in the development of children's dietary behavior practices. It is recommended clinical practice that pediatric nurse practitioners (PNPs) assess readiness to change behavior in parents of overweight or obese children. The components of the Prochaska and DiClemente's Transtheoretical Model (TTM) in correlation with the counseling style of Rollnick, Miller and Butler's methods of Motivational …

An Evaluation Of Methicillin Resistant Staphylococcus Aureus Survival On Five Environmental Surfaces Under Two Different Humidities, With And Without The Addition Of Bovine Serum Albumin, Courtney Ann Coughenour

UNLV Theses, Dissertations, Professional Papers, and Capstones

The spread of antibiotic resistant bacteria is a major public health concern, as they result in greater healthcare costs and increased morbidity and mortality rates. Methicillin resistant Staphylococcus aureus (MRSA) is one organism of particular concern, with the number of infections increasing in epidemic proportion. Bacterial surface contamination with MRSA is significant, as it may serve as a reservoir for transmission and have negative health implications. The purpose of this study was to evaluate survival of MRSA on five environmental surface materials; glass, wood, vinyl, plastic, and cloth. The effect of relative humidity (RH) and bovine serum albumin (BSA) were …

An Evaluation Of Plastic Toys For Lead Contamination In Day Care Centers In The Las Vegas Valley, Joseph Alan Greenway

UNLV Theses, Dissertations, Professional Papers, and Capstones

The harmful effect of childhood exposure to environmental lead continues to be a major health concern. This study examined lead contamination within the plastic of children's toys. It was also hypothesized that the use of lead as a stabilizer would result in higher incidents of elevated lead (> 600 ppm) in polyvinyl chloride plastics (PVC) than non-PVC plastics. It was also hypothesized that, due to the use lead chromate, yellow toys would have higher incidents of elevated lead (> 600 ppm) than toys of other colors. Toy samples were limited to those found in day care centers in Las Vegas, …

Evaluation Of X-Ray Fluorescence (Xrf) Lead Detection Method For Candy, Ashley Marie Phipps

UNLV Theses, Dissertations, Professional Papers, and Capstones

The harmful effects of childhood exposure to environmental lead continue to be a major health concern. Due to the significance of this hazard, a Healthy People 2010 objective was set to reduce all young children's blood lead levels to less than 10 micrograms per deciliter. Identification and removal of lead-contaminated candies is an integral part of the primary prevention of lead poisoning in children.

This research examined the efficacy of a protocol to use a portable XRF to screen candies for lead contamination. Method Detection Limits (MDLs) of 5.45 ppm and 7.05 ppm were determined in the Bulk Sample and …

Molecular Epidemiology Of Methicillin-Resistant Staphylococcus Aureus (Mrsa), Ilene Bautista

UNLV Theses, Dissertations, Professional Papers, and Capstones

Staphylococcus aureus is a frequent cause of a myriad of infections in hospitals, healthcare facilities and community settings. It is often related to skin and soft tissue infection, but it can also cause other diseases varying from food poisoning to endocarditis and toxic shock syndrome. According to a study by Kuehnert et al. (2006) there has been a strong association between Staphylococcus aureus (S. aureus) carriage and risk factors for subsequent infections. Subsequent infection from S. aureus , including methicillin-resistant S. aureus (MRSA) infection, can be projected by looking at the presence of S. aureus nasal colonization. The purpose of …

The Effect Of Concurrent Cell Phone Use And Walking On Gait Characteristics, Jennifer Marie Aldridge

UNLV Theses, Dissertations, Professional Papers, and Capstones

This study examined the effects that cell phone use has on gait and situation awareness. Subjects (n=18; 24.67±3.60 yrs) completed three five-minute treadmill walking conditions (self-selected speed): (1) CONTROL (walking only), (2) TALK (walking and talking), and (3) TEXT (walking and texting). The number of detected lights presented in the visual field was recorded, as were kinematic data of the feet. Using a one-way repeated measure ANOVA it was determined light detection (F _(2,17) =39.777, p<0.001) and TC_min (F_(2,17) =8.574, p=0.001) were different between conditions. Specifically, light detection decreased during TEXT (2.6±2.2 lights) versus TALK (5.8±0.05 lights, p<0.001) or CONTROL (5.9±0.05 lights, p<0.001); TC_min decreased …

Photochemical Delivery Of Bleomycin In Malignant Glioma Cells, Joseph William Blickenstaff Iii

UNLV Theses, Dissertations, Professional Papers, and Capstones

Despite continued efforts, glioblastoma multiforme (GBM) remains an incurable form of primary brain cancer. Surgical resection followed by radiation treatment and chemotherapy have been the most effective modalities in prolonging median survival time to 14 months. This research aims to study the response of two different cell culture models to a novel drug delivery method termed photochemical internalization (PCI). PCI is a technique to improve the utilization of macromolecules (e.g. the chemotherapeutic drug bleomycin) in site-specific cancer therapy. This concept is based on the use of specially designed photosensitizers (e.g. aluminum phthalocyanine disulfonate; AlPcS _2a ) which preferentially localize in …

Mercury Concentrations In Muscle Tissue From Sportfish In Lake Mead, Nevada, Joanna L. Kramer

UNLV Theses, Dissertations, Professional Papers, and Capstones

Lake Mead is the largest reservoir by volume in the United States and provides fishing opportunities for numerous anglers. Considerable attention has been given to the bioaccumulation of methylmercury in fish tissues, however, no formal study utilizing approved USEPA methodology has been conducted to quantify the amount of mercury present in fish tissue from Lake Mead. The purpose of this study is to determine the concentrations of mercury present in the most commonly consumed fish from Lake Mead and to identify if any of the 4 major basins contain fish with elevated concentrations of mercury. Largemouth bass (n=49), striped bass …

Women's Health And Fitness Magazines: An Accurate Portrayal?, Jennifer M. Shymansky

UNLV Theses, Dissertations, Professional Papers, and Capstones

This study is a content analysis examining the representation of health information, particularly the leading causes of death, in cover story headlines of one year of the top women's health and fitness magazines: Prevention , Shape , Fitness , Self , Health and Women's Health. The study is grounded in social responsibility theory, a normative media theory. The findings show that women's health and fitness magazines' cover story headlines predominately discuss diet and exercise for weight loss, rather than the leading causes of death facing women. This under representation of actual societal health concerns can limit the vast readerships' health …

Asthma Severity In School-Children And The Quality Of Life Of Their Parents, Noelle S. Cerdan

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background . The everyday management of a child with asthma can affect the parent's quality of life. Past studies which examined the effects of asthma characteristics of the child on parental quality of life have not reached a consensus over findings. Few studies examine parent characteristics such as mental health and sociodemographics on the quality of life of parents of asthmatic children.

Purpose . To examine the effect of asthma severity of school-children and sociodemographic characteristics onthe caregiver's quality of life. It also investigates whether agreement exists between the caregiver's perception of asthma severity and physician-diagnosed asthma severity.

Methodology . …

Investigation Of Magnetic Resonance Imaging And Spectroscopy For The Detection Of Breast Cancer, Robert Thomas Etnire

UNLV Theses, Dissertations, Professional Papers, and Capstones

Magnetic resonance imaging (MRI) of the breast offers an alternative to screening mammography which may benefit those women at high risk for breast cancer, women under the age of 40, and those with dense breast tissue. One concern with MRI is the number of high false positives. Coupling MRI with magnetic resonance spectroscopy(MRS) may lower the number of false positives, and thus improve the diagnostic capabilities of MRI for the clinician. MRS for breast imaging focuses on the total choline containing compounds in the spectra in the suspected breast lesion to analyze areas of concern. The results of the study …

Using Thermoluminescent Dosimeters To Measure The Dose From High And Low Energy X-Ray Sources, Wesley Leon Boyd

UNLV Theses, Dissertations, Professional Papers, and Capstones

This work focused on using thermoluminescent dosimeters (TLDs) to measure the doses from two x-ray sources, a CT scanner and a linear accelerator (linac). In addition ion chambers were used to determine the isodose contours from the operation of the linac. 1mm x 1mm x 1mm TLD-100 microcubes were placed in a Lawrence Livermore National Laboratory (LLNL) torso phantom and irradiated using a Toshiba Aquilion One 320-Slice CT Scanner. Different thicknesses of shielding composed of antimony and bismuth powder suspended in an ethylene copolymer were used to shield the phantom and the dose reduction was measured. The dose reduction ranged …

Predictors Of Hpv Knowledge And Awareness In Rural America, Melanee Dulfon

UNLV Theses, Dissertations, Professional Papers, and Capstones

Prior research has shown that overall awareness of HPV is low among women in America. Yet limited research exists as to the awareness of HPV among rural U.S. women. The goal of this study was to determine the factors that predict women's knowledge and awareness of HPV in rural America. Secondary data was utilized from the National Cancer Institute Health Information National Trends Survey (HINTS) of 2005. The sample consisted of 788 adult women over the age of 18 from rural areas of the U.S. Results indicated younger age, increased education, receipt of a Pap exam in over three years, …

An Art Therapy Approach To Addressing Burnout Prevention Among Pediatric Healthcare Providers, Hoilam Tang

EVMS School of Health Professions Theses & Dissertations

The purpose of this thesis was to develop an art therapy based burnout prevention program for pediatric healthcare providers who work directly with chronically and/or terminally ill children. This program will run for a period of four months. Eight sessions were designed to implement self-care skills, promote a supportive working environment and professional boundaries, address grief and loss, and process work-related stress and distress in order to reduce burnout and compassion fatigue among pediatric healthcare providers. This program was designed for 5 to 7 healthcare providers who have similar experiences and work duties. This program will run as a peer …

Exploring American Indian Adolescents’ Needs Through A Community-Driven Study, Emily Gordon Basto

EVMS School of Health Professions Theses & Dissertations

The current higher rates of substance use and abuse for Native American adolescents compared to non-Natives is an issue that requires attention. There are a variety of risk factors that lead to this current situation, including discrimination and acculturation pressures. Past treatment options have been limited with American Indians and have not incorporated community involvement. Art therapy has been utilized in substance abuse programs and with American Indian populations; however, art therapy has been limited in taking a community-driven approach. As art therapy and community involvement are seen as culturally relevant, together they may serve as a model for assessing …

Stories, Ethics And The Interpretation Of Meaning: Bearing Witness To Mothers' Stories Of Their Neonatal Intensive Care Unit Experience, Angela Chisum Blackburn

Dissertations

This study grounded in narrative perspectives was conducted to uncover mothers' experience of having a baby in the Neonatal intensive Care Unit (NICU). The purpose of this study was to describe and interpret mothers NICU experiences, and to sensitize health care professionals about the importance of mothers' personal experience stories.

The NICU experience began with mothers' birth experience or the incident that led up to her infant requiring care in the NICU and her experience extended beyond the NICU with future concerns about the health and wellbeing of her baby.

Stories of mothers' experience were gleaned from data generated from …

Intuitive Eating And Its Relationship With Physical Activity Motivation, Amy Campbell Nielson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Research has shown that restrictive eating, or dieting, can be devastating to one's health. A new paradigm, intuitive eating, suggests that individuals eat based off of their physiological cues, and not for emotional or social ones. To date, restrictive eating has been extensively researched, but intuitive eating has not. The main purpose of this study was to examine the relationship between intuitive eating and its relationship between physical activity motivation and physical activity maintenance, using the self-determination theory.

Participants completed a survey to determine their intuitive eating level, their physical activity motivation, and their physical activity maintenance (n = …

An Epistemological Checkup: The Explication, Application, And Evolution Of An Integrated Theory Of Couples Therapy, Mckenzie Leigh Christensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

When clinicians practice therapy without a clear theoretical foundation, they lack direction and purpose. When training to be a marriage and family therapist, understanding and clarifying one's integrated theory and practice of therapy is essential. This mixed methods study was designed to elucidate and apply my theory of therapy in order to identify fidelity to my model as well as understand the effects it has on clients. This study also focused on how experiences in each session of therapy influenced the next session.

Three couples who presented for therapeutic services at the Utah State University Marriage and Family Therapy clinic …

The Chakra Energy System And Art Therapy: A Heuristic Study, Linda Scalise

EVMS School of Health Professions Theses & Dissertations

The chakra system, an Eastern philosophy relating to an individual’s health, energy, and consciousness, has been integrated into the healing practices of psychology, meditation, yoga, and alternative medicine. Dale (2005), Judith (1996), and Myss (1996) described the chakra energy system as focal points embedded within the human energy system that regulate all components of the self. Researchers in complementary therapies and energy medicine have long been interested in how the integration of this system affects the progression of disease, human behavior, and emotional disturbances. However, although rich in symbolism and metaphor, no literature has been found demonstrating the use of …

The Role Of Metnase In Dna Replication Fork Stress Response And Dna Damage, Leyma Pérez De Haro

Biomedical Sciences ETDs

Metnase is a recently evolved human protein with methylase (SET) and nuclease domains that is widely expressed, especially in proliferating tissues. Metnase promotes plasmid and viral DNA integration, and through an interaction with topoisomerase IIα (TopoIIα) it promotes chromosome decatenation. Metnase interacts with DNA ligase IV, promotes non-homologous end-joining (NHEJ), and repression causes mild hypersensitivity to ionizing radiation. TopoIIα has a proposed role in relaxing positive supercoils in front of replication forks. NHEJ factors have been implicated in the replication stress response. Here we show that Metnase promotes cell proliferation, but does not affect replication fork elongation as measured by …

Role Of Mmps And Timp-3 In Neuronal Death And White Matter Injury Following Transient Global Ischemia, Espen Jacob Walker

Biomedical Sciences ETDs

Transient global ischemia to the mouse results in hypoxic hypoperfusion to the brain and can lead to regional vulnerability, inflammation, and cell death. Characterization of this model with varying occlusion and reperfusion times allowed us to provide evidence for a consistent injury to the hippocampus and white matter regions of the brain. Following 30 minutes of bilateral carotid artery occlusion (BCAO) and 7 days of reperfusion, hippocampal injury to the CA2 region was consistently identified with neuronal loss and fluorojade positive cells. Three days following reperfusion, white matter injury was demonstrated by increased astrocyte reactivity and oligodendrocyte loss. With a …

Regulation Of N-Formyl Peptide Receptor Trafficking And Signaling By Arrestins, Brant Michael Wagener

Biomedical Sciences ETDs

This work focuses on how arrestin regulates trafficking and signaling of the N-formyl peptide receptor (FPR), a G protein-coupled receptor (GPCR). GPCRs are involved in almost all physiologic processes and numerous pathologic processes. There is an intimate relationship between GPCR trafficking and signaling that controls many cellular processes. However, the protein-protein interactions that control post-endocytic trafficking and signaling of GPCRs are poorly understood. Our previous reports demonstrated that three events take place upon FPR activation in the absence of arrestins: accumulation of FPR in the perinuclear recycling endosome, lack of FPR recycling and apoptosis. All of these phenotypes were rescued …