Medicine and Health Sciences Commons^™

Alternative Regulation Of The Alginate Algd Operon By An Activated Algb In Nonmucoid Pseudomonas Aeruginosa Is Dependent On Sigma 54, Jean Kim

Theses and Dissertations

Alginate overproduction by Pseudomonas aeruginosa, which causes a mucoid phenotype, is a major virulence factor associated with chronic pulmonary infections in cystic fibrosis patients. Expression of the algD operon for alginate biosynthesis requires three major regulators in association with the ECF sigma factor, σ22, in mucoid strains that are typically defective in anti-sigma factor, MucA. One such algD regulator is AlgB, a member of the NtrC family of two-component systems, which typically utilize σ54. However, neither σ54 nor the cognate sensor kinase (KinB) of AlgB are required for algD expression in such mucoid strains. I hypothesized that KinB-phosphorylated AlgB must …

In-Vitro Metabolism And Protein Binding Of 5-Hmf, A Potential Antisickling Agent, Taghrid Obied

Theses and Dissertations

Purpose. 5-HMF is a potential antisickling agent forming a Schiff-adduct with hemoglobin (Hb). In-vitro studies were designed to identify the metabolic pathways of 5-HMF in human hepatic cytosol, to assess inter-species differences in its hepatic metabolism, and to predict in-vivo PK properties. Moreover, metabolism of 5-HMF in human RBCs was investigated. Finally, in-vitro studies were done to characterize 5-HMF binding kinetics with human Hb and albumin (HSA). Methods. NAD+ reduction was monitored at 340 nm in human hepatic cytosol for 5-HMF (26 mM) and prototypical ADH and ALDH substrates in the presence or absence of their inhibitors. Furthermore, concentration-dependency studies …

Prediction Of Human Systemic, Biologically Relevant Pharmacokinetic (Pk) Properties Based On Quantitative Structure Pharmacokinetic Relationships (Qspkr) And Interspecies Pharmacokinetic Allometric Scaling (Pk-As), Prajakta Badri

Theses and Dissertations

This research developed validated QSPKR and PK-AS models for predicting human systemic PK properties of three, preselected, pharmacological classes of drugs, namely opioids, β-adrenergic receptor ligands (β-ARL) and β-lactam antibiotics (β-LAs) using pertinent human and animal systemic PK properties (fu,, CLtot, Vdss, fe) and their biologically relevant unbound counterparts from the published literature, followed by an assessment of the effect of different molecular descriptors on these PK properties and on the PK-AS slopes for CLtot and Vdss from two species (rat and dog). Lipophilicity (log (D)7.4) and molecular weight (MW) were found to be the most statistically significant and biologically …

Development Of Novel Ahr Antagonists, Hyosung Lee

University of Kentucky Doctoral Dissertations

Aryl hydrocarbon receptor (AHR) is a sensor protein, activated by aromatic chemical species for transcriptionally regulating xenobiotic metabolizing enzymes. AHR is also known to be involved in a variety of pathogenesis such as cancer, diabetes mellitus, cirrhosis, asthma, etc. The AHR signaling induced by xenobiotics has been intensively studied whereas its physiological role in the absence of xenobiotics is poorly understood. Despite a number of ligands of AHR have been reported thus far, further applications are still hampered by the lack of specificity and/or the partially agonistic activity. Thus, a pure AHR antagonist is needed for deciphering the AHR cryptic …

Investigation Of Speech Samples From Typically Developing Preschool Age Children: A Comparison Of Single Words And Imitated Sentences Elicited With The Paba-E, Matthew William Olsen

Dissertations and Theses

Assessment of speech sound production in young children provides the basis for diagnosis and treatment of speech sound disorders. Standardized single-word articulation tests are typically used for identification of speech sound errors because they can provide an efficient means of obtaining a speech sample for analysis and comparison to same-age peers. A major criticism of single-word articulation tests is that they may not accurately reflect speech sound production abilities in conversation. Comparison of performance in single-word and conversational contexts has produced conflicting results in the available research.

The purpose of the present study was to compare speech samples obtained using …

Observer-Dependent Model For Analyzing Subjective Parameters In Epidemiology, Milad Zarei

Open Access Theses & Dissertations

Although medical technologies for preventing the contagion and spread of infectious diseases have improved steadily throughout the last century, new infectious diseases are still emerging and spreading swiftly. The modeling of infectious disease spread is crucial in addressing the lack of predictive ability in epidemiology. Managing the spread of infectious diseases requires processing quantitative epidemiological data and the ability to capture the dynamics of the infectious disease in order to provide a measure of control.

In this thesis, I have introducing cognitive biases in diseases spread modeling. For the first time, to the author's knowledge, the human subjective experience has …

Neighborhood Deprivation, Neighborhood Aculturation, And The Retail Food Environment In A U.S.-Mexico Border Urban Area, Teresa Mercedes Anchondo

Open Access Theses & Dissertations

The prevalence of obesity in the United States is increasing at a rapid rate, and is the result of an imbalance of caloric intake and expenditure. Obese individuals are at significantly greater risk for coronary artery disease, high blood pressure, diabetes, and cancer, as compared to non-obese individuals. Both individual correlates and neighborhood conditions contribute to the risk for obesity. For example, family history, race/ethnicity, and residence in a low socioeconomic status neighborhood are all associated with increased risk of obesity. The retail food environment is one aspect of low socioeconomic status neighborhoods that may contribute to increased obesity risk. …

Vocabulary Acquisition Of Bilingual Students Through The Implementation Of Dialogic Shared Storybook Reading Techniques, Samantha Kamille Ariaz

Open Access Theses & Dissertations

Children who are learning English as a second language and whose caregivers speak only Spanish were chosen to participate in the study. Parents were trained using Dialogic Shared Storybook Reading (DSSR) techniques (Whitehurst, 1988). The study was a single subject multiple baseline design across behaviors. Reading conditions were counterbalanced to control for acquisition of new vocabulary. There were a total of five participants, three children and two mothers (n=5). The participants were separated into two families. Each family was exposed to three reading book conditions while the parents implemented the specified techniques: 1. Shared reading with an electronic, bilingual Spanish/ …

Experimental Study Of The Effects Of Green Tea, Alejandra Avila

Open Access Theses & Dissertations

Background. Leishmaniasis is a parasitic disease caused by an intracellular parasite belonging to the genus Leishmania. The cutaneous form of the disease causes often significant disfigurement, accelerates progression to clinical AIDS and tuberculosis, and is associated with a number of adverse economic, psychosocial, and nutritional consequences. An estimated 350 million persons are at risk for this globally distributed disease and 1.5-2 million new cases occur annually. Leishmaniasis is endemic in 88 countries on five continents. It is distributed throughout most of the Americas ranging from southern Texas to northern Argentina. It is also regarded as a threat to the readiness …

The Relationship Between Soft Body Composition And Bone Mineral Density In Premenopausal Hispanic And Caucasian Women, Clarelouise Anita Highfield

Open Access Theses & Dissertations

Investigating the relationship between soft body composition and bone mineral density of premenopausal Hispanic and Caucasian women may help in determining strategies to lower the risk of osteoporosis in postmenopausal women. PURPOSE: To investigate the relationship between BMD and soft tissue body composition, including lean mass and fat mass, of premenopausal Caucasian and Hispanic women. METHODS: Participants were 76 Hispanic [mean ± SD age: 42.7 ± 4.6 y; ht: 161.3 ± 5.66 cm; body mass: 70.14 ± 15.09 kg; BMI: 26.92 ± 15.09 kg/m2] and 46 White [age: 43.8 ± 4.3 y; ht: 165.5 ± 6.16 cm; body mass: 66.37 …

A Specific Regulatory Role For Sgtα On The Maturation And Activation Of Steroid Hormone Receptors, Atanu Paul

Open Access Theses & Dissertations

Steroid hormone receptors (SHRs) are ligand-dependent transcription factors belonging to the nuclear receptor superfamily. These receptors regulate various physiological functions in higher ordered eukaryotes. In the absence of hormone these receptors form a complex with molecular chaperones such as Hsp90 and Hsp70 and other cochaperones in the cytoplasm. Association with the Hsp90-Hsp70 chaperone machinery leads to the maturation of the Ligand Binding Domain (LBD) of the receptors and enables the receptors to bind hormone with high affinity. Upon binding with a specific hormone the receptors are translocated into the nucleus where they initiate transcription of specific genes. Although the involvement …

The Latina/O Mental Health Paradox Or Racial/Ethnic Disparities? Depression Among New Immigrants To The U.S, Sandra Iveth Ramirez

Open Access Theses & Dissertations

The Latino/a Mental Health Paradox states that the foreign-born immigrants have better mental health outcomes when compared with native-born groups of various ethno-racial backgrounds, with the gap in mental stress minimizing overtime. The objective of this study is to examine whether Latina/os immigrants suffer an ethno-racial disparity in mental health or if they have favorable mental health outcomes as predicted by the Paradox. Expanding on the Latina/o Mental Health Paradox, rather than comparing Latina/o immigrants with native-born, this study will compare depression among recently legalized Latina/os with recent legalized European immigrants.

Health Risk And Health-Seeking Behaviors Of Migrant And Seasonal Farmworkers On The Us-Mexico Border, Claudia Delilah Saenz

Open Access Theses & Dissertations

Background and Significance

The estimated 1–3 million migrant and seasonal farmworkers (MSFW) employed in the U.S. play a crucial role in assuring the success of the multi-billion dollar agricultural industry and the nation's access to a safe and nutritious food supply. This predominantly Mexican immigrant occupational group is documented to be at–risk for chronic and infectious disease morbidity and premature mortality but published studies are limited which describe their reproductive/sexual health, mental health, substance abuse, and workplace violence situation. Prior studies are especially scant regarding U.S.–Mexico border MSFW.

Objectives and Hypothesis

The major objective of the pilot study was to …

Factors Related To The Recruitment And Retention Of Nurse Practitioners In Rural Areas, Dayle Boynton Sharp

Open Access Theses & Dissertations

For the past several decades, rural America has experienced significant health disparities. Changes in the demographics of rural locations in the U.S. have led to a) an increase in an aging population, b) an increase in minority populations, and c) a large number of unemployed individuals due to the shifting of jobs. In some locations, a large number of these unemployed or underemployed are uninsured or underinsured. Parallel to the changes in demographics, there has been a decrease in primary care providers in rural areas. Consequently, rural America is facing reduced and unequal access to healthcare. One solution to limited …

The Co-Existence Of Diabetes Mellitus Type 2 And Depression Symptoms In Mexican American Adults: Its Relation To Glucose Control, Perceived Stress And Physical Health, Guillermina Rincon Solis

Open Access Theses & Dissertations

Type 2 diabetes is a common chronic metabolic disease affecting 23.6 million people (7.8%) in the U.S. and is the 6th leading cause of death among adults (CDC, 2007). Mexican-Americans, the largest group within the Hispanic U.S population, have double the rate of type 2 diabetes. Long-term glucose control (HbA1C) is essential to lessen the risk of diabetes complications; self-care demands for diabetes management may be overwhelming and creates stress that predisposes individuals to depression and affects long-term glucose control (Anderson et al., 2000; Black, 1999; Black, Markides, & Ray, 2003). The purpose of this study was to evaluate the …

Understanding Environmental Health Disparities Of Roma Populations In Romania, Jennifer L. Hall

Dissertations and Theses @ UNI

The Roma are the largest and most marginalized minority group of Central and Eastern Europe (CEE) with the highest number residing in Romania. Their problems and struggles are multi-faceted and deeply rooted in a long history of discrimination and stigmatization. Over the years, many strategies have been developed and implemented across CEE, yet very few studies have addressed their environmental and occupational health issues. Moreover, because the vast majority of these studies have employed quantitative methodology, they have had the unfortunate effect of dehumanizing and devaluing the Roma people through scientific reductionism.

The purpose of this study was to gain …

Impact Of The Iowa Smoke-Free Ban Among The College Students Of The University Of Northern Iowa, Jean Marie Vianney Hirwa Kavamahanga

Dissertations and Theses @ UNI

The purpose of this study was to measure the effects of the Iowa Smoke-Free Ban impact on: smoking behavior, knowledge of the ban and knowledge of smoking health effect, reported smoking in both indoor and outside venues; support for smoke-free public venues; and self-reported effects of the law on consumption and quitting. A non-random sample of 113 students (49.44% male and 50.44% female) participated in this study.

The 35-item questionnaire was used to evaluate the impact of the Iowa smoke-free ban among college students of the University of Northern Iowa. Descriptive statistics were used to analyze the survey responses. The …

The Role Of The Methyl Dna Binding Domain Protein 2 (Mbd2) In Breast Cancer, Omar Mian

Theses and Dissertations

Methyl-CpG Binding Proteins (MCBPs) are thought to function as the interpreters of epigenetic information encoded in cytosine methylation. Their ability to translate DNA methylation into local transcriptional repression has sparked interest in the role of Methyl-Binding Domain Proteins (MBDs) in cancer, where repatterning of CpG methylation is common. In this dissertation I summarize and discuss observations made in the Ginder Lab linking MCBPs to the progression of neoplastic disease. It is clear from our work that the Methyl Binding Domain Protein 2 (MBD2) is necessary for the persistent repression of critical tumor suppressor genes in breast cancer. We show that …

Dendritic Spine Density Varies Between Unisensory And Multisensory Cortical Regions, Moazzum Bajwa

Theses and Dissertations

In the brain, the dendritic spine is a point of information exchange that extends the neuronal surface on which synapses occur, as well as facilitates and stabilizes those contacts. Furthermore, dendritic spines dynamically change in shape and number in response to a variety of factors. Dendritic spine numbers are reduced in mental retardation, enhanced during development, sensory enrichment or physical exercise, or fluctuate during the reproductive cycle. Thus, for a given neuron type, it might be expected that dendritic spine number might achieve a dynamic optimum. Indeed, many studies of spine density of pyramidal neurons in sensory cortex indicate that …

The Prognostic Potential Of The Epidermal Growth Factor Receptor And Nuclear Factor Kappa B Pathways And Associated Therapeutic Strategies In Patients With Squamous Cell Carcinoma Of The Head And Neck, Pamela Wirth

Theses and Dissertations

Little is known about the signaling pathways that contribute to treatment response in advanced stage head and neck tumors. Increased expression of epidermal growth factor receptor (EGFR) and downstream pathways such as nuclear factor kappa B (NFκB) are implicated in aggressive tumor phenotypes and limited response to therapy. This study explored the rationale for combining the proteasome inhibitor bortezomib with the EGFR inhibitor gefitinib in a subset of head and neck squamous cell carcinomas with high EGFR gene amplification. Drug responses of gefitinib and bortezomib as single agents and in combination within head and neck squamous cell carcinoma cell lines …

Discrimination Of Tone Contrasts In Mandarin Disyllables By Naïve American English Listeners, Shari Salzhauer Berkowitz

Dissertations, Theses, and Capstone Projects

The present study examined the perception of Mandarin disyllabic tones by inexperienced American English speakers. Participants heard two naturally-produced Mandarin disyllables, and indicated if the two were the same or different. A small native Mandarin-speaking control group participated as well. All 21 possible Mandarin contrasts where the initial syllable varied but the final syllable stayed the same were tested. Acoustic analysis was performed on the stimuli under study. Mandarin subjects scored at ceiling on all contrasts. American English subjects performed poorly on contrasts where the difference in mean F0 was small, or where the difference in the offset F0 of …

Body Type And Perceived Physical Competence In College Female Dancers, Brenna Marie Vogel

Dissertations and Theses @ UNI

The relationship between body type and perceived physical competence was determined among college female company dancers. Participants were from one of four technique based dance companies at a midwestern university. They were questioned as to their perceived physical competence in seven different styles of dance, all of which had been offered at the selected university over the previous academic year. Body type was calculated using both the Body Mass Index Scale and the Body Composition Analyzer, which is a bioelectrical impedance analysis of one's body fat percentage, total body water, and body weight. A total of 44 participants took part …

The Relationship Between Post Reach Exit Exam (E2) Failure Remediation And Nclex-Rn Success Of Graduates Of Baccalaureate Nursing Programs, Patricia Gale Allen

Student Theses and Dissertations

An ex post facto study was conducted to determine whether any relationship exists between remediation post Reach Exit Exam (E2) failure and NCLEX-RN success of graduates of baccalaureate nursing programs. Data was gathered from responses to the seventh annual validity study (V7S) offered to deans and directors of nursing programs by the Director of Research for Elsevier, Incorporated. Data focused on test groups which received administration of the E2 between September 1, 2006 and August 31, 2007. Responses from 28 baccalaureate nursing programs were included in the study. After considering each program offering of either version one, version two or …

Predicting Flavonoid Ugt Regioselectivity With Graphical Residue Models And Machine Learning., Arthur Rhydon Jackson

Electronic Theses and Dissertations

Machine learning is applied to a challenging and biologically significant protein classification problem: the prediction of flavonoid UGT acceptor regioselectivity from primary protein sequence. Novel indices characterizing graphical models of protein residues are introduced. The indices are compared with existing amino acid indices and found to cluster residues appropriately. A variety of models employing the indices are then investigated by examining their performance when analyzed using nearest neighbor, support vector machine, and Bayesian neural network classifiers. Improvements over nearest neighbor classifications relying on standard alignment similarity scores are reported.

Comparison Of Ammonia And Particulate Matter Air Sample Concentrations At Task-Locations Within Swine Confinement Buildings., Earl Dan Bembry

Electronic Theses and Dissertations

Introduction: A task-associated analysis of ammonia and particulate matter concentrations was conducted on swine farms in breeding and gestation barns. The purpose of this study was to determine if specific tasks performed by workers increase concentrations of ammonia and particulate matter exposure.

Methods: An exploratory, descriptive design was used to determine if swine confinement workers have increased ammonia and particulate matter exposure when performing assigned tasks in breeding and gestation barns. A convenience sample of 8 workers volunteered from among all breeding and gestation workers (n=24). Data collection occurred in the morning at each of the 8 farms …

Soothability And Growth In Preterm Neonates, Holly Johanna Diesel

Dissertations

This proposal seeks to understand the relationship between soothability, weight gain and length of hospital stay in premature infants when the infants receive a simple, non-invasive treatment to help them cope with the noxious environment of the special care nursery. Thousands of premature infants are born every year in the United States, and the numbers are increasing. Prematurity is the leading cause of mortality in infants. Despite numerous technological and medical advances in treatment and care, premature infants still have difficulty adapting to life outside the uterus as a result of immature nervous systems and significant differences in the pre …

Rx For Change: Nurses' Response To A Smoking Cessation Intervention, Laura Louise Bisch Ochoa

Dissertations

Problem: An evaluation of a smoking cessation educational intervention for direct care RNs. Design: A randomized controlled trial. Methods: The study was conducted at a large Midwestern academic medical center. Direct care Registered nurses (dcRN’s) employed at least .7 FTE and spending 80% of their time in direct patient care on general medicine or general surgery units were recruited. Recruitment occurred between April and May, 2009. Outcome data was abstracted from charts of patients receiving care from the intervention group. Interventions: Participants were randomized to either ‘Rx for Change’ which was a training program regarding tobacco cessation or to a …

Antibiotic Use By Members Of The American Association Of Endodontics: A National Survey For 2009- A Follow Up From The Report In 1999, Pye Kyu

Theses and Dissertations

The purpose of this study was to determine the changes in prescribing habits of active members of American Association of Endodontics (AAE) with regards to antibiotics in comparison to the findings reported by Yingling et al. in 1999. The invitations to take the online survey were sent via email to 2593 active members. A response rate of 37.75% was obtained. It was determined to be adequate for analysis and for comparison to the results obtained by Yingling et al. Comparisons between the percentages shown in this survey and the previous survey were tested using a z-test. An ANOVA model was …

A Head Injury Teaching Module For Pre-Hospital Assessment: Using The Four Score, Chris A. Wolf

Theses and Graduate Projects

The purpose of this project is to create a teaching module and evaluation tool for a United States Air Force Aeromedical Evacuation Squadron for the pre-hospital assessment of patients with head injuries using the FOUR Score assessment scale. Specifically, this project integrates theory, relevant literature, and reflection on service to and caring for others. Information from published literature supports the development and implementation of a new assessment tool, such as the FOUR Score, to assess patients who have suffered a head injury. Ultimately, a teaching module is presented, described, and evaluated, along with potential questions to be addressed in the …

Strengthening Nurse/Physician Collaborative Relationships To Promote Patient Safety, Julie Lynch Weegman

Theses and Graduate Projects

The purpose of this project is to identify implementable best practices that promote effective nurse physician collaboration in the hospital setting at the unit level for strengthening the culture of patient safety. These best practices will be shared with nurses and physicians at a workshop using the methods of powerpoint presentation and appreciative inquiry. The goal for this workshop is to inspire nurses and physicians to spread the seeds of collaborative practice throughout the organization through the path of shared governance.