Medicine and Health Sciences Commons^™

Analysis Of The Transcriptional Promoter Which Regulates The Latency-Related Transcript Of Bovine Herpesvirus 1, Clinton J. Jones, G. Delhon, A. Bratanich, G. Kutish, D. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

As a transcriptional promoter in primary cultures of sensory ganglionic neurons, DNA sequences near the 5' terminus of the latency-related (LR) gene of bovine herpesvirus 1 were at least 10 times more efficient than the simian virus 40 early promoter-enhancer. In contrast, as a promoter in bovine, rodent, or monkey cells, the LR promoter was approximately six times less efficient than the simian virus 40 early promoter-enhancer. The LR promoter had strict orientation preferences in neurons and all other mammalian cell lines tested. Removal of a 146-base-pair XhoI fragment from the LR promoter resulted in stimulation of LR promoter …

Plasma Native And Peptidase-Derivable Met-Enkephalin Responses To Restraint Stress In Rats. Adaptation To Repeated Restraint, K. Pierzchala, G. R. Van Loon

Internal Medicine Faculty Publications

Met-enkephalin and related proenkephalin A-derived peptides circulate in plasma at picomolar concentration as free, native pentapeptide and at nanomolar concentration in cryptic forms. We have optimized conditions for measurement of immunoreactive Met-enkephalin in plasma and for generation by trypsin and carboxypeptidase B of much greater amounts of total peptidase-derivable Met-enkephalin in plasma of rats, dogs, and humans. Free Met-enkephalin (11 pM) is constituted by native pentapeptide and its sulfoxide. Characterization of plasma total Met-enkephalin derived by peptidic hydrolysis revealed a small amount (38 pM) of Met-enkephalin associated with peptides of molecular mass less than 30,000 D, and probably derived from …

Pms And Eosinophilia-Myalgia Syndrome, Mary Guinan

School of Public Health Faculty Research

No abstract provided.

Murine Gamma Interferon Fails To Inhibit Toxoplasma Gondii Growth In Murine Fibroblasts., Joseph D. Schwartzman, Steven L. Gonias, E R. Pfefferkorn

Dartmouth Scholarship

Although treatment of human macrophages or fibroblasts with human gamma interferon results in the inhibition of intracellular Toxoplasma gondii, murine gamma interferon stimulated only murine macrophages, not murine fibroblasts, to inhibit T. gondii. This species difference may be important in understanding the control of acute and chronic toxoplasmosis.

Identification And Characterization Of The Herpes Simplex Virus Type 2 Gene Encoding The Essential Capsid Protein Icp32/Vp19c, S P Yei, S I Chowdhury, B M Bhat, A J Conley, W S Wold, W Batterson

Faculty, Staff and Student Publications

We describe the characterization of the herpes simplex virus type 2 (HSV-2) gene encoding infected cell protein 32 (ICP32) and virion protein 19c (VP19c). We also demonstrate that the HSV-1 UL38/ORF.553 open reading frame (ORF), which has been shown to specify a viral protein essential for capsid formation (B. Pertuiset, M. Boccara, J. Cebrian, N. Berthelot, S. Chousterman, F. Puvian-Dutilleul, J. Sisman, and P. Sheldrick, J. Virol. 63: 2169-2179, 1989), must encode the cognate HSV type 1 (HSV-1) ICP32/VP19c protein. The region of the HSV-2 genome deduced to contain the gene specifying ICP32/VP19c was isolated and subcloned, and the nucleotide …

The Role Of Calcium Ions In Toxic Cell Injury., John L. Farber

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Calcium ions have been increasingly implicated as a mediator of the mechanisms generating lethal cell injury under a variety of pathologic circumstances. An overview of the various roles suggested for such alterations in cellular calcium homeostasis is presented. The central role of plasma membrane damage in the genesis of irreversible cell injury is used to divide the postulated roles for calcium ions into two major mechanisms. On the one hand, calcium ions have been proposed as mediators of the functional consequences of plasma membrane injury. An influx of extracellular calcium ions across a damaged permeability barrier and down a steep …

Ethical Reasoning And Analysis: The Elements, Martin Benjamin

Center for the Study of Ethics in Society Papers

Presented to the WMU Center for the Study of Ethics in Society February 10, 1989.

The Effects Of Decreased Uncertainty And Telescopic Versus Full Field Training On The Useful Field Of View, Lauren M. Jackson

Masters Theses & Specialist Projects

The “Useful Field of View” (UFOV) is the entire area in which information can be gathered without moving the eyes or head (Ball, Beard, Roenker, Miller & Griggs, 1998). Previous research has demonstrated that the UFOV shrinks with age (Scialfa, Kline & Lyman, 1987; Plude & Doussard-Roosevelt, 1987: Ball et al., 1988). With decrement in the UFOV, everyday activities, such as driving and walking can be limited. If the area in which information is received is smaller, then objects seem to appear suddenly and here is little time to react to them. One example of an everyday activity that would …

Susceptibility Of Hepatic Microcirculation To Reperfusion Injury: A Comparison Of Adult And Suckling Rats., Alan M. Yahanda Md, Facs, C N Paidas, M G Clemens

Other Specialties

Primary graft failure and vascular thromboses are frequent complications of liver transplantation, yet the mechanisms responsible remain unclear. Previous work from our laboratory has shown that hepatic reperfusion injury results in damage at the microvessel level. The present study was performed to determine whether an increased susceptibility of immature animals to microvascular injury during reperfusion might be a contributing factor in these complications. Suckling (35 to 50 g) or adult (250 to 400 g) rats were subjected to 30 or 60 minutes of hepatic ischemia to the left and median lobes followed by 90 minutes of reperfusion. Control animals were …

Ciliary Microtubule Capping Structures Contain A Mammalian Kinetochore Antigen, J. M. Miller, W. Wang, Ron Balczon, W. L. Dentler

University Faculty and Staff Publications

Structures that cap the plus ends of microtubules may be involved in the regulation of their assembly and disassembly. Growing and disassembling microtubules in the mitotic apparatus are capped by kinetochores and ciliary and flagellar microtubules are capped by the central microtubule cap and distal filaments. To compare the ciliary caps with kinetochores, isolated Tetrahymena cilia were stained with CREST (Calcinosis/phenomenon esophageal dysmotility, sclerodactyly, telangiectasia) antisera known to stain kinetochores. Immunofluorescence microscopy revealed that a CREST antiserum stained the distal tips of cilia that contained capping structures but did not stain axonemes that lacked capping structures. Both Coomassie blue-stained gels …

Ua12/2/1 Magazine, Wku Student Affairs

WKU Administration Documents

Special magazine edition of the College Heights Herald.

• Albrecht, Dana. Nocturnal Nurse – Jo Ann Blink
• Hall, David. Traumas of Divorce

Ua12/2/1 College Heights Herald, Vol. 65, No. 34, Wku Student Affairs

WKU Administration Documents

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news. This issue contains articles:

• Poynter, Chris. Pearce-Ford Tower Dwellers Asking for Breath of Fresh Air
• Howard, Laura. Still Here, Paul Cook’s Duties Mount
• Lockert, Anya. Officials Blame ’83 Act for Low Ratio of Blacks – Desegregation Plan
• Regents Ask for Students’ Opinions
• Bricking, Tanya. More Cooks to Stir Recipe for Budget – Thomas Meredith
• Committee Will Study Athletic Reports
• Rubber Ducky Business Has Holiday Boom
• Wessling, Susan. Group Works to Ban Public Smoking
• Students Need AIDS Education
• Chattin, John. Editorial Cartoon – AIDS Skeleton
• Bright Ideas Projecting New Image …

Ua12/2/1 College Heights Herald, Vol. 65, No. 33, Wku Student Affairs

WKU Administration Documents

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news. This issue contains articles:

• Jones, Angie. Students Warned AIDS Will Be Here
• Poynter, Chris. Therapist Wilson Whitehead Shows Clients How to Relax
• Summers, Kaye. Physical Plant, Faculty Senate Protest Insurance Plan
• Bricking, Tanya. New Ideas for Efficiency Committee Will Pay for Bright Ideas
• Gifts to Western Exceed $6 Million
• Tenure Hearing Set for Weekend – Gabe Buntzman
• Deaf Can Communicate with Legislators
• Two Students Arrested on Assault Charges
• Search Committees Need Efficiency
• Chattin, John. Editorial Cartoon – Big Red Searching
• Black, Sam. Ku Klux Klan Protestor Questions Motives
• McMahon, …

Ua12/2/1 College Heights Herald, Vol. 65, No. 32, Wku Student Affairs

WKU Administration Documents

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news. This issue contains articles:

• Bricking, Tanya. Wallace Wilkinson Scores with College Plan
• Lockert, Anya. 300 Pay Homage to Fallen Leader Martin Luther King
• Howard, Laura. Higher Education Funding Depends on Tax Bill
• Black, Sam. Surge in Auto Break-Ins Inflates Campus Crime Numbers
• Wessling, Susan. Five Department Heads Resign
• AIDS Presentation to be Held Monday
• Thomas Meredith to Work for the Arts
• Associated Student Government Changes WKU Glasgow Constitution
• Ausenbaugh, Laura. Non-Traditional Students to Get Scholarships
• Wallace Wilkinson Getting Priorities Straight
• Chattin, John. Editorial Cartoon – Governor in Troubled Waters …

Oral Drug Retaining Device, Jesse B. Douglas Jr.

Oral Health Science Faculty Patents

An oral drug retaining device includes a receptacle for receiving and holding a polymer matrix impregnated with a pharmacologic agent. The receptacle is formed by a cooperating basket and frame. The basket and frame include a hinge at one side and a latch pin at the opposite side to allow the receptacle to be selectively opened and closed to replace the polymer matrix as desired. Cooperating male and female connectors are provided to allow the receptacle to be releasably mounted to a tooth of a patient. One of these connectors is adapted for mounting to a tooth while the other …

Serial Conditioning As A Function Of Stimulus, Response, And Temporal Dependencies, William L. Palya, Rick A. Bevins

Department of Psychology: Faculty Publications

Six experiments were used to examine the effects of explicit response, stimulus, and temporal dependencies on responding in an interfood interval. The first two experiments demonstrated that 10- segment 60-s interfood clocks controlled similar distributions of key pecking in pigeons regardless of whether response-reinforcement contiguity was required, allowed, or precluded. The third and fourth experiments found that in the absence of an explicit response-reinforcement dependency, systematic explicit stimuli in an interfood interval were sufficient to establish and maintain the characteristic distribution of key pecking and that an interval without an explicit clock failed to establish or maintain key pecking. The …

A Baseline Case: The Woburn Wells G And H Superfund Site, Steven Konkel

Environmental Health Science Faculty and Staff Research

This section profiles the types of disputes which arose at the Woburn Wells G and H Superfund site in Woburn, Mass. The Woburn Wells G and H case serves as a baseline because it demonstrates how obstacles cause delays and impasses. This section contains an overview of what has transpired since the discovery of contaminants in two of the town municipal wells, Well G and H.

Pharmacokinetics Of Cefepime In Patients With Respiratory Tract Infections, J. M. Kovarik, J. C. Ter Maaten, C. M. A. Rademaker, M. Deenstra, I. M. Hoepelman, H. C. Hart, Gary R. Matzke, J. Verhoef

Pharmacotherapy and Outcomes Science Publications

The steady-state pharmacokinetics of cefepime were evaluated in 10 middle-aged and elderly patients with acute lower respiratory tract infections who were receiving 1 g intravenously every 12 h. One preinfusion and 15 postinfusion serum samples and total urine output were collected over one dosing interval between days 3 and 8 of therapy. Cefepime concentrations in serum over time exhibited a multicompartmental profile. Peak and trough concentrations in serum determined by a validated high-performance liquid chromatography method were 71.2 +/- 17.2 (mean +/- standard deviation) and 6.0 +/- 4.9 mg/liter, respectively. The steady-state volume of distribution was 0.22 +/- 0.05 liter/kg. …

Apparent Biliary Pseudolithiasis During Ceftriaxone Therapy, Karen L. Heim-Duthoy, Erskine M. Caperton, Robert Pollock, Gary R. Matzke, Dirk Enthoven, Phillip K. Peterson

Pharmacotherapy and Outcomes Science Publications

Biliary pseudolithiasis has been reported in patients who received ceftriaxone therapy. To examine this phenomenon further, serial gallbladder sonograms were evaluated in 44 adult patients who received intravenous ceftriaxone at 2 g or a placebo daily for 14 days in a double-blind controlled study. Ultrasound examinations of gallbladders were performed on days 1 and 14 of therapy and 2 weeks posttherapy if abnormalities were observed on day 14. Eight patients were unevaluable because of abnormal base-line gallbladder sonograms. Thirty-six patients (ceftriaxone, n = 28; placebo, n = 8) demonstrated normal baseline gallbladder sonograms and were evaluated for the development of …

Two Alkaline Phosphatase Genes Are Expressed During Early Development In The Mouse Embryo, Ann C. Hahnel, Daniel A. Rappolee, Jose Luis Millan, Thomas Manes, Carol A. Ziomek, Nicoleta G. Theodosiou, Zena Werb, Roger A. Pedersen, Gilbert A. Schultz

Department of Obstetrics and Gynecology Research Publications

Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue …

Disorders Of The Digestive-System In The Elderly, Robert D. Shamburek, John T. Farrar

Internal Medicine Publications

CHANGES in the anatomy and physiology of the epithelium of the digestive organs because of aging are slight.1 The functional capacity of both the secretory and absorptive cells of the gut is so great that a decrease to as little as 5 to 10 percent of normal function is required for a clinical effect to be evident. In contrast to the epithelial-cell reserve, connective-tissue changes are manifested in midlife, and age-related changes in these tissues are responsible for many digestive disorders, such as colonic vascular ectasias and diverticula of the gut.

The anatomical and physiologic changes that do occur …

Cyclosporine-Induced Sympathetic Activation And Hypertension After Heart-Transplantation, Urs Scherrer, Susan F. Vissing, Barbara J. Morgan, Julia A. Rollins, Richard S. A. Tindall, Steves Ring, Peter Hanson, Pramod K. Mohanty, Ronald G. Victor

Internal Medicine Publications

BACKGROUND.

Hypertension is a frequent complication of cyclosporine-induced immunosuppression, but the underlying mechanism is unknown. In anesthetized animals, the administration of cyclosporine increases sympathetic-nerve discharge, which may contribute to hypertension.

METHODS.

To determine whether cyclosporine-induced hypertension is accompanied by sustained sympathetic neural activation in patients, we recorded sympathetic action potentials using intraneural microelectrodes (in the peroneal nerve) in heart-transplant recipients receiving azathioprine and prednisone alone (n = 5) or in combination with cyclosporine (n = 14). We performed the same studies in eight patients with myasthenia gravis who were receiving cyclosporine and eight who were not, in five patients with …

Assessment Of Cefazolin And Cefuroxime Tissue Penetration By Using A Continuous Intravenous Infusion., John E. Connors, Joseph T. Dipiro, Ronald G. Hayter, K. Dale Hooker, Johnny A. Stanfield, Timothy R. Young

Publications from the Office of the Dean

A continuous intravenous infusion was used to assess the tissue penetration of cefazolin (14 subjects) and cefuroxime (15 subjects) in orthopedic surgery patients. Subjects were randomly assigned to receive a continuous intravenous infusion of cefazolin (mean, 178.6 mg/h) orcefuroxime (mean, 330.0 mg/h) at a rate estimated to achieve a target steady-state total concentration of 50 micrograms/ml in serum. The infusion was initiated 12 to 14 h before surgery, and blood and muscle tissue samples were collected intraoperatively at the times of incision and wound closure. Although there was a significant difference between the free concentrations ofcefazolin (at incision, 9.3 micrograms/ml; …

Information Interface - Volume 14, Issue 1 - January/February 1990, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

Management Of Swallowing Disorders: A Program For Professionals Working In Rural Areas, Kathleen D. Sims Mcsd/Ccc-Slp, Sue Reinsel-Garcia Otr/L, Kathy M. Love Ma/Ccc-Slp, Elizabeth Kohler Ed.D., O.T.R., University Of Montana Rural Institute

Health and Wellness

Research indicates that 74% of all nursing home patients experience eating difficulties sometime during their stay (Trupe, Siebens, & Siebens, 1984). Additionally, 59% of patients suffering from stroke experience some degree of dysphagia and aspiration difficulties (Echelard, Thoppil, & Melvin 1984). A significant number of the high risk dysphagia patients described above suffer from life threatening aspiration pneumonia. Consequently the management of swallowing disorders (Dysphagia) is of critical concern to hospital and nursing home personnel. Patients specficially at risk for dysphagia, according to recent studies, include those with head injury, stroke (CVA), and cerebral palsy. Also, patients experiencing cancer of …

The Effects Of Passive Warming On Muscle Injury, Timothy Strickler, Terry Malone, William E. Garrett

Peer Reviewed Articles

This study investigated the effects of passive warming on the biomechanical properties of the musculotendinous unit. Paired tibialis anterior (TA) and extensor digitorum longus (EDL) muscles in the rabbit hindlimb were passively heated to different temperatures and then subjected to controlled strain injury. The parameters examined were: 1) percent increase in length to failure, 2) force to failure, 3) energy absorbed by the musculotendinous unit to failure, and 4) site of failure. Warmed (39°C ± 0.5°C) TA (P ≤ 0.01) and EDL (P ≤ 0.05) muscles achieved a greater increase in length from rest before failing than did their contralateral …

Effects Of Magnesium-Aluminum Hydroxide And Calcium Carbonate Antacids On Bioavailability Of Ofloxacin, Soledad Flor, David R. P. Guay, John A. Opsahl, Kenneth Tack, Gary R. Matzke

Pharmacotherapy and Outcomes Science Publications

The effects of 15- and 5-ml doses of magnesium-aluminum hydroxide (MAH) and calcium carbonate (CC) antacids, respectively, on the bioavailability of ofloxacin after single oral 400-mg doses of ofloxacin were investigated in a 32-subject, randomized, crossover, open-label study. On four separate occasions, subjects received ofloxacin alone or antacid 24 h before, 2 h before, or 2 h after ofloxacin administration (n = 16 for each antacid). CC administration had no significant effect on the rate and extent of ofloxacin absorption regardless of the timing of antacid administration. A small but significant negative effect of MAH administration 2 h before ofloxacin …

Ada Constitution & Bylaws (1990), American Dental Association

Constitution & Bylaws

The ADA Commons Constitution & Bylaws archival collection comprises printed issues of the American Dental Association's Constitution and Bylaws. The collection also includes the ADA Charter and the ADA Code of Ethics issued 1924-1946.

Pharmaceuticals And Intellectual Property: Meeting Needs Throughout The World, Thomas G. Field Jr.

Law Faculty Scholarship

To the extent that most people think about patents and other forms of intellectual property at all, they tend to be aware that the owners of such property may have the legal capacity to limit market entry--without fully appreciating the extent to which products or processes that can be easily copied might otherwise be unavailable. Focusing on their function in recouping risk capital, this article will survey the types and functions of intellectual property. Then it will attend to the situation in developing countries, particularly the role of intellectual property in meeting their needs for medical products.

Ada Code Of Ethics (January 1990), American Dental Association

Code of Ethics

The ADA Code of Ethics has three main components: The Principles of Ethics, the Code of Professional Conduct and the Advisory Opinions. Contents may also include: Amendment to ADA Principles of Ethics and Code of Professional Conduct and Insert for the ADA Principles of Ethics and Code of Professional Conduct. The most current issue is available on the ADA’s website.