Medicine and Health Sciences Commons^™

Application Of Dna Fingerprints For Cell-Line Individualization, Dennis A. Gilbert, Yvonne Reid, Mitchell H. Gail, David Pee, Christine White, Robert J. Hay, Stephen J. O'Brien

Biology Faculty Articles

DNA fingerprints of 46 human cell lines were derived using minisatellite probes for hypervariable genetic loci. The incidence of 121 HaeIII DNA fragments among 33 cell lines derived from unrelated individuals was used to estimate allelic and genotypic frequencies for each fragment and for composite individual DNA fingerprints. We present a quantitative estimate of the extent of genetic difference between individuals, an estimate based on the percentage of restriction fragments at which they differ. The average percent difference (APD) among pairwise combinations from the population of 33 unrelated cell lines was 76.9%, compared with the APD in band sharing …

Laminin Potentiates Differentiation Of Pcc4uva Embryonal Carcinoma Into Neurons, T. M. Sweeney, Roy C. Ogle, C. D. Little

School of Medical Diagnostics & Translational Sciences Faculty Publications

The embryonal carcinoma PCC4uva differentiates into neurons in response to treatment with retinoic acid and dbcAMP. We used this in vitro model system to study the effects of laminin on early neural differentiation. Laminin substrata markedly potentiate neural differentiation of retinoic acid and dbcAMP-treated cultures. Only laminin induced more rapid neural cell body clustering, neurite growth and neurite fasciculation as compared to type IV collagen, type I collagen, and fibronectin substrata. Exogenous laminin substrata promoted greater cell attachment, cellular spreading and growth to confluence than type IV collagen, type I collagen, fibronectin and glass substrata. Laminin-induced effects were inhibited by …

The Changing Role Of Prevention In Dental Education., Brian M. Lange, David G. Dunning, Graham Shea

Journal Articles: College of Dentistry

No abstract provided.

Information Interface - Volume 14, Issue 4 - August/September 1990, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

[Review Of] The American Medical Association Handbook Of First Aid & Emergency Care, Robert A. Aken

Library Faculty and Staff Publications

No abstract provided.

The Effect Of Oxygen On Corneal Neovascularization, M. A. Culton, D. B. Chandler, A. D. Proia, D. W. Hickingbotham, G. K. Klintworth

Osteopathic Medicine, Jerry M. Wallace School of

Since tissue oxygen levels are believed to play a pivotal role in new vessel growth in several situations, we studied the effect of several oxygen concentrations (0, 10, 21, 50, 75, or 100%) on corneal vascularization induced in the rat by chemical cautery. We achieved this by perfusing known concentrations of oxygen through goggles fitted over both eyes of the rat after corneal cauterization. Neovascularization was measured in flat corneal preparations with India ink-filled vessels 4 days postcautery using computerized image analysis. The angiogenic response of rats whose eyes were continuously exposed to 0-75% oxygen were not significantly different from …

Inability Of Mitogen-Activated Lymphocytes Obtained From Patients With Malignant Primary Intracranial Tumors To Express High Affinity Interleukin 2 Receptors, Lucinda H. Elliott, W. H. Brooks, T. L. Roszman

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Patients with primary malignant brain tumors manifest a variety of abnormalities in cell-mediated and humoral immunity. Diminished T cell reactivity has been shown in these patients to be linked to deficiencies in interleukin 2 (IL-2) production that cannot be overcome by exogenous IL-2. In this study, specific binding of radiolabeled IL-2 to PHA-stimulated lymphocytes from brain tumor patients demonstrates that the number of high affinity interleukin 2 receptors (IL-2R) is greatly reduced. FACS analysis indicates that the relative density of the p55 protein (Tac protein) is lower on the mitogen-activated lymphocytes obtained from patients than on comparably treated lymphocytes from …

Psychological Effects Of Disaster On Children And Their Families: Hurricane Hugo And The Loma Prieta Earthquake, Judith A. Boore, Gina Earle, Lewis Aptekar

FMHI Publications

No abstract provided.

Higher-Order Discrimination, Adrian M. S. Piper

Center for the Study of Ethics in Society Papers

Presented to the WMU Center for the Study of Ethics in Society January 22, 1990.

The Growth Of Simian Virus 40 (Sv40) Host Range/Adenovirus Helper Function Mutants In An African Green Monkey Cell Line That Constitutively Expresses The Sv40 Agnoprotein., Terryl P. Stacy, Michele Chamberlain, Susan Carswell, Charles N. Cole

Dartmouth Scholarship

The simian virus 40 T-antigen carboxy-terminal mutants, dlA2459 and dlA2475, are cell line and temperature dependent for growth and plaque formation in monkey kidney cells. Although these mutants did form plaques on BSC-1 cells at 37 degrees C, they were about fivefold less efficient for plaque formation than wild-type simian virus 40. These mutants did not grow in CV-1 cells and did not synthesize agnoprotein in those cells. CV-1 cells which constitutively express the agnoprotein were permissive for mutant plaque formation. However, late mRNAs, virion proteins, and progeny virion yields did not accumulate to wild-type levels during mutant infection of …

Jefferson Alumni Bulletin – Volume Xxxix, Number 4, Summer 1990

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume XXXIX, Number 4, Summer 1990

Dermatology: new diseases, new cures, page 8

Treatment and prevention in the national fight against drugs, page 10

Frontiers in thoracic surgery, page 14

Three portraits are presented, page 17-19

Added perspectives on dermatology, page 20

A Health Sciences Library Liaison Project To Support Biotechnology Research, Gregory F. Pratt

Library Staff Publications

No abstract provided.

The Effect Of Oxygen On Corneal Neovascularization, M. Culton, D. B. Chandler, A. D. Proia, D. Hickingbotham, G. K. Klintworth

Osteopathic Medicine, Jerry M. Wallace School of

No abstract provided.

Adapting The Health Knowledge Inventory For Use With High School Seniors, Cara Case

Masters Theses & Specialist Projects

Knowledge alone is not enough to ensure that the individual will act in a healthy manner or choose positive health behaviors. However, knowledge may enable one to engage in sound health practices. Health education must respond to the changes in American culture by developing approaches that achieve maximum communication and learning. The purpose of this thesis is to assess the suitability of the Health Knowledge Inventory – High School Version (HKI-HS) for testing with high school seniors. A field test was performed to assess whether the HKI-HS is an appropriate measurement instrument of personal health knowledge among high school seniors. …

1990 Commencement For Jefferson Medical College And College Of Graduate Studies

Sidney Kimmel Medical College Commencements

No abstract provided.

Effect Of Dietary Aluminum Sulfate On Calcium And Phosphorus Metabolism Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

The effect of dietary aluminum sulfate on Ca and P metabolism was studied using 1-day-oldmale broiler chicks. In Experiment 1, practical diets providing .90% Ca plus .45% available P (P_av), .90% Ca plus .78% P_av, 1.80% Ca plus .45% P_av, or 1.80% Ca plus .90% P_av were fed with 0 or .392% Al as aluminum sulfate for 21 days. The control diet (.90% Caplus .45% P_av) without added A1 was fed to all chicks during Days 22 to 49. In general, Al significantly (Pi), tibia breaking strength, tibia weight, percentage of …

Relationship Of Dietary Aluminum, Phosphorus, And Calcium To Phosphorus And Calcium Metabolism And Growth Performance Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

Dietary treatments providing three levels of added Al (0, .196, or .392%) as aluminum sulfate and of available phosphorus (P_av) (.45, .68, or .78%) in a factorial arrangement were administered to day-old chicks in Experiment 1. Plasma inorganic phosphorus (P_i) was significantly (P < .05) elevated by increasing P_av and was decreased by Al. Body weight gain, feed intake, and the gain:feed ratio at Day 21 were significantly decreased by increased concentrations of Al, but were unaffected by the P_av concentrations. Decreases of 39 and 73% in weight gain and of 34 and 66% in feed intake resulted from feeding …

Mechanism Of Escape Of Endogenous Murine Leukemia Virus Emv-14 From Recognition By Anti-Akr/Gross Virus Cytolytic T Lymphocytes., Hillary D. White, Michael D. Robbins, William R. Green

Dartmouth Scholarship

It was previously shown that spleen cells from endogenous ecotropic murine leukemia virus emv-14+ AKXL-5 mice fail to stimulate an anti-AKR/Gross virus cytolytic T-lymphocyte (CTL) response in a mixed lymphocyte culture with primed C57BL/6 responder spleen cells, whereas spleen cells from AKXL strains carrying the very similar emv-11 provirus do stimulate a response (Green and Graziano, Immunogenetics 23:106-110, 1986). We wished to determine whether the lack of response with AKXL-5 spleen cells was at the level of recognition between effector cell and target cell and whether the relevant mutation was within the emv-14 provirus. It is shown here that EMV-negative …

Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …

Nccaa Men's Track & Field National Meet Top Performances, Cedarville College

Men's and Women's Track & Field Statistics (1984-1995)

No abstract provided.

[Review Of] The Potent Male: Fact, Fiction, Future, Robert A. Aken

Library Faculty and Staff Publications

No abstract provided.

1990 Servicemaster National Outdoor Track & Field Championships, Cedarville University

Men's and Women's Track & Field Programs

No abstract provided.

Ua66/4/2 Ceremony Of Capping & Pinning, Wku Dental Hygiene

WKU Archives Records

Program from capping & pinning ceremony.

Ua66/14/3 Nursing Pinning Ceremony, Wku Nursing

WKU Archives Records

Pinning ceremony program listing graduates of the WKU Nursing program.

Vocal Recognition Of Pups By Mother Mexican Free-Tailed Bats, Tadarida Brasiliensis Mexicana, Jonathan P. Balcombe

Sentience Collection

The ability of Mexican free-tailed bat mothers and pups to recognize vocalizations of their presumptive kin (pup isolation calls and mother echolocation calls, respectively) was tested using playbacks of recorded calls. Captive individuals were presented with calls of two bats, one presumptive kin and the other a stranger, from opposite sides of a circular wire arena. Response was determined by amount of time spent on each side of the arena, time spent in contact with a cloth bat model in front of each speaker, and number of separate contacts with each model. For the latter two measures, mothers showed a …

Information Interface - Volume 14, Issue 3 - May/June 1990, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

Save A Life- Diagnose Subarachnoid Haemorrhage!, Rashid Jooma, Iqtidar H. Bhatti

Section of Neurosurgery

No abstract provided.

A Comparison Of Smoking Cessation Methods In Normal Smokers And Smokers With Chronic Obstructive Pulmonary Disease, Mary Beth Mueller

Open Access Master's Theses (through 2010)

This research was designed to investigate whether there would be a difference in mean exhaled carbon monoxide levels and mean number of cigarettes smoked for two randomized groups of smokers.Smoking cessation therapy was manipulated in smokers with a chronic, productive cough (COPD) and those who were asymptomatic (normal). Individual therapy was compared to self-help therapy.The main purpose of this study was an attempt to define the smoking cessation therapy that worked best for each of these two groups.With the information gained from this research, it was hoped that educating different types of smokers (those with and without lung disease) about …

Methods Used To Self-Predict Ovulation A Comparative Study, Richard Fehring

College of Nursing Faculty Research and Publications

Time of ovulation as detected by a self-test of luteinizing hormone (LH) in the urine was compared with time of ovulation as detected by self-observation of cervical mucus. Twenty regularly cycling women monitored their cervical mucus and urine LH for two complete menstrual cycles. Of the cycles that had an LH surge, 100% were on the peak day of cervical mucus or within three days before the peak day. Self-observation of cervical mucus, therefore, can be an accurate method of determining optimal fertility.

1990 District 22 Track & Field Championships Final Results, Cedarville College

Men's and Women's Track & Field Statistics (1984-1995)

No abstract provided.