Medicine and Health Sciences Commons^™

Co-Crystal Structure Of The Fusobacterium Ulcerans Ztp Riboswitch Using An X-Ray Free-Electron Laser., Christopher Jones, Brandon Tran, Chelsie Conrad, Jason Stagno, Robert Trachman, Pontus Fischer, Alke Meents, Adrian Ferré-D'Amaré

School of Medicine and Health Sciences Student Works

Riboswitches are conformationally dynamic RNAs that regulate gene expression by binding specific small molecules. ZTP riboswitches bind the purine-biosynthetic intermediate 5-aminoimidazole-4-carboxamide riboside 5'-monophosphate (ZMP) and its triphosphorylated form (ZTP). Ligand binding to this riboswitch ultimately upregulates genes involved in folate and purine metabolism. Using an X-ray free-electron laser (XFEL), the room-temperature structure of the Fusobacterium ulcerans ZTP riboswitch bound to ZMP has now been determined at 4.1 Å resolution. This model, which was refined against a data set from ∼750 diffraction images (each from a single crystal), was found to be consistent with that previously obtained from data collected at …