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Articles 31 - 60 of 88
Full-Text Articles in Medicine and Health Sciences
Immunokinetics Of Theileria Equi Specific Antibodies: A Comparison In Serial And Single Dilution Elisa Antibody End Titres, Yogesh Kumar, Dharam Vir Malhotra, Anil Kumar Nichani, Ashok Kumar, Shruti Dhar, Sanjay Kumar
Immunokinetics Of Theileria Equi Specific Antibodies: A Comparison In Serial And Single Dilution Elisa Antibody End Titres, Yogesh Kumar, Dharam Vir Malhotra, Anil Kumar Nichani, Ashok Kumar, Shruti Dhar, Sanjay Kumar
Turkish Journal of Veterinary & Animal Sciences
Theileria equi, a haemoprotozoan of equids, is responsible for endemic infection/latency in tropical/subtropical parts of the world. The present study investigated the immunokinetics of T. equi infection in experimental animals and the diagnostic applicability of single dilution ELISA vis-à-vis conventional serial dilution ELISA. Sequential serum samples were collected up to 90 days postinfection (PI) from 4 donkeys experimentally infected with T. equi. Immunokinetics of T. equi antibodies end titre were determined by serial and single dilution ELISA. A significant rise in initial ELISA antibody end titre (1:800 in serial dilution ELISA and from 1:600 to 1:650 in single dilution ELISA) …
Evaluation Of Canine Anti-Leishmania Igg Subclasses And Their Relation With Skin Signs In Naturally Infected Dogs In The Northwest Of Iran, Ali Shabestari Asl, Majid Sharifi, Mehdi Mohebali, Zabihollah Zarei, Zohreh Babaloo, Behnaz Akhoundi
Evaluation Of Canine Anti-Leishmania Igg Subclasses And Their Relation With Skin Signs In Naturally Infected Dogs In The Northwest Of Iran, Ali Shabestari Asl, Majid Sharifi, Mehdi Mohebali, Zabihollah Zarei, Zohreh Babaloo, Behnaz Akhoundi
Turkish Journal of Veterinary & Animal Sciences
Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG_1 and IgG_2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of …
Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras
Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras
Turkish Journal of Veterinary & Animal Sciences
Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (\alpha, \beta, \epsilon, and \iota) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 …
Presence Of Infectious Pancreatic Necrosis Virus On Rainbow Trout (Oncorhynchus Mykiss) By Histopathology, Elisa And Rt-Pcr, Gabriel Aguirre-Guzmán, Ned Iván Iván De La Cruz-Hernández, Jesús Genaro Sánchez-Martínez
Presence Of Infectious Pancreatic Necrosis Virus On Rainbow Trout (Oncorhynchus Mykiss) By Histopathology, Elisa And Rt-Pcr, Gabriel Aguirre-Guzmán, Ned Iván Iván De La Cruz-Hernández, Jesús Genaro Sánchez-Martínez
The Thai Journal of Veterinary Medicine
Trout (Oncorhynchus mykiss) culture in Mexico experiences sporadic and significant fish mortalities, wheredisease signs are associated with infectious pancreatic necrosis virus disease (IPNV). The purpose of the present workwas to support the potential of ELISA, histopathology, and RT-PCR as routine techniques for IPNV detection.Fourteen trout farms were monitored after a disease outbreak. Positive results were confirmed in different collectedorganisms from 21% of tested farms by all 3 techniques. Virus detection by ELISA and RT-PCR was successfullyperformed in only two days after the initial signs of disease were observed in fish, since these methods are fasterwhen compared to histopathology, which only …
Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey, Meryem Aydemi̇r Atasever, Mustafa Atasever, Korhan Özturan
Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey, Meryem Aydemi̇r Atasever, Mustafa Atasever, Korhan Özturan
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the presence and levels of aflatoxin M1 (AFM1) in yoghurt and ayran consumed in the province of Erzurum, Turkey. For this purpose, a total of 80 yoghurt samples and 80 ayran samples were randomly obtained from markets. The samples' AFM1 content and concentrations were examined by competitive ELISA method. AFM1 at detectable level (5 ng/kg) was found in 87.5% of the yoghurt samples and in 90.0% of the ayran samples. AFM1 levels in 16 (20%) yoghurt and in 11 (13.6%) ayran samples exceeded the maximum tolerable limit of the Turkish Food Codex. The …
Molecular And Serological Evidence For Anaplasma Infection In Yaks, Miling Ma, Zhijie Liu, Guiquan Guan, Youquan Li, Jianxun Luo, Hong Yin
Molecular And Serological Evidence For Anaplasma Infection In Yaks, Miling Ma, Zhijie Liu, Guiquan Guan, Youquan Li, Jianxun Luo, Hong Yin
Turkish Journal of Veterinary & Animal Sciences
Bovine anaplasmosis caused by Anaplasma marginale is considered to be an important disease in China; large-scale investigations have not been carried out since the 1990s, however, and whether or not yaks can be infected by A. marginale has never been investigated. In this study, 2 yaks were successfully infected by the inoculation of blood infected with A. marginale under experimental conditions. Furthermore, an investigation of 482 field serum samples from yaks using an enzyme-linked immunosorbent assay (ELISA) with the recombinant major surface protein 5 (MSP5) showed that 84.4% of the results were positive. In consideration of previously determined ecological and …
Aflatoxin M1 Contamination In Cow’S Milk In Kayseri (Central Turkey), Hi̇kmet Mavuş Buldu, A. Nedret Koç, Güven Uraz
Aflatoxin M1 Contamination In Cow’S Milk In Kayseri (Central Turkey), Hi̇kmet Mavuş Buldu, A. Nedret Koç, Güven Uraz
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine the levels of aflatoxin M1 (AFM1) in the milk of cows in the villages of Kayseri province, Turkey. Ninety milk samples were investigated for AFM1 contamination using the enzyme linked immunoassay (ELISA). The mean value of AFM1 levels was 59.9 ng/L, 95% confidence interval was 54.4-65.5 ng/L, and standard deviation was 26.6 ng/L. For 63 samples (70%) the values were significantly above the limit permitted by European Union criteria (EU) and the Turkish Food Codex (TFC) (P < 0.05). However, the differences in AFM1 levels between villages were not significant (P > 0.05). In conclusion, since the level of AFM1 in the milk of cows was higher …
Possible Role Of Toxoplasmosis In Patients With First-Episode Schizophrenia*, Mehmet Tanyüksel, Özcan Uzun, Engi̇n Araz, Özgür Koru, Cahi̇t Babür
Possible Role Of Toxoplasmosis In Patients With First-Episode Schizophrenia*, Mehmet Tanyüksel, Özcan Uzun, Engi̇n Araz, Özgür Koru, Cahi̇t Babür
Turkish Journal of Medical Sciences
To determine the possible relationship between toxoplasmosis and patients with first-episode schizophrenia (FES). Materials and methods: Seventy-three subjects with FES (15-54 years old; mean: 23.4 years) and 40 healthy individuals (20-54 years old; mean: 30.3 years) were enrolled in the study. Most of the FES patients (90.4%) and the control individuals (95.0%) were male. Specific IgG and IgM antibodies to Toxoplasma gondii were investigated by enzyme-linked immunosorbent assay (ELISA), and the total amount of antibodies was investigated with the Sabin-Feldman dye test (SFDT). Results: Compared to the control individuals, study subjects had significantly more toxoplasmosis-like symptoms and more cats in …
Maedi-Visna Virus In Turkish Sheep: A Preliminary Serological Survey Using Elisa Tests, Silvia Preziuso, Mehmet Erman Or, Monica Giammarioli, Abdullah Kayar, Francesco Feliziani, Remzi̇ Gönül, Silvana Farneti, Cağla Parkan Yaramiş, Carlo Valente, Vincenzo Cuteri
Maedi-Visna Virus In Turkish Sheep: A Preliminary Serological Survey Using Elisa Tests, Silvia Preziuso, Mehmet Erman Or, Monica Giammarioli, Abdullah Kayar, Francesco Feliziani, Remzi̇ Gönül, Silvana Farneti, Cağla Parkan Yaramiş, Carlo Valente, Vincenzo Cuteri
Turkish Journal of Veterinary & Animal Sciences
The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. The aims of this study were as follows: i) to verify the diagnostic performance of a home-made ELISA test (HM-ELISA) using Turkish ovine sera in comparison with other commercially available ELISA tests, and ii) to perform a preliminary epidemiological survey in İstanbul province. The MVV seroprevalence in İstanbul province was 15.3%. Older sheep were 5 times more likely to be positive than younger animals, ewes were 3 times more likely to be positive than rams, and all the …
Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa
Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa
The Thai Journal of Veterinary Medicine
This study was to conduct a surveillance of Mycoplasma synoviae (MS) infection in mixed Thai native chickens. Samples were submitted from 30 mixed Thai native chicken flocks, 15 birds per flock, aged between 1-4.5 months in the area of Nakornpathom province in the September 2005-October 2006 period. Each bird was bled for MS serology by serum plate agglutination (SPA) and enzyme linked immunosorbent assay (ELISA) test kits and swabbed for MS antigen detection by polymerase chain reaction (PCR) technique. Results revealed that the positive reactors detected by the SPA test, ELISA and PCR procedure were 12, 9 and 8 flocks, …
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Turkish Journal of Veterinary & Animal Sciences
Lung, trachea, and serum samples obtained from turkeys showing clinical signs of respiratory disease were examined for the presence of Bordetella avium infection (bordetellosis) using culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The material of this study consisted of 2 live turkeys at the age of 24 weeks with respiratory distress, nasal discharge, and cough that had been brought to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Adnan Menderes University. The results of the study show that B. avium, the causative agent of bordetellosis, was not isolated from either the lung or trachea, while both …
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Turkish Journal of Veterinary & Animal Sciences
Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples …
Evaluation Of Serological Tests For The Diagnosis Of Visceral Leishmaniasis, Selçuk Kiliç, Ayşegül Taylan Özkan, Cahi̇t Babür, Gönül Tanir, Henk Dfh Schallig
Evaluation Of Serological Tests For The Diagnosis Of Visceral Leishmaniasis, Selçuk Kiliç, Ayşegül Taylan Özkan, Cahi̇t Babür, Gönül Tanir, Henk Dfh Schallig
Turkish Journal of Medical Sciences
Aim: Visceral leishmaniasis (VL) is a zoonosis that affects both animals and man. VL is seen sporadically in the Aegean, Mediterranean, Black Sea and central Anatolian regions of Turkey. The aim of this study was to evaluate serological tests for the diagnosis of VL. Materials and Methods: The performance of two agglutination tests based on freeze-dried whole promastigote Leishmania antigen, i.e. Fast Agglutination Screening Test (FAST) and Direct Agglutination Test (DAT), an Indirect Immunofluorescent Antibody Test (IFAT), and an Enzyme-Linked Immunosorbent Assay (ELISA) using soluble antigen were compared to parasitological diagnosis in the serum specimens of 59 patients with the …
Surveillance Of Mycoplasma Gallisepticum Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Thawat Lekdamrongsak
Surveillance Of Mycoplasma Gallisepticum Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Thawat Lekdamrongsak
The Thai Journal of Veterinary Medicine
This study was to conduct a surveillance of Mycoplasma gallisepticum (MG) infection in mixed Thai native chickens. Samples were submitted from 30 mixed Thai native chicken flocks, 15 birds per flock, aged between 1 and 4 months without MG vaccination in the area of Nakornpathom province during the September 2005 - October 2006 period. Each bird was bled for MG serology by a serum plate agglutination (SPA) test and enzyme linked immunosorbent assay (ELISA) test kits and swabbed for MG antigen detection by polymerase chain reaction (PCR) technique. Results revealed that the positive reactors detected by the SPA test, ELISA …
Development Of A Highly Sensitive Elisa For Quantification Of Hepatitis B Virus (Hbv) Surface Antigen (Hbsag), Resul Karakuş, Lati̇fe Arzu Aral, Bi̇lkay Baştürk, Cemaletti̇n Aybay
Development Of A Highly Sensitive Elisa For Quantification Of Hepatitis B Virus (Hbv) Surface Antigen (Hbsag), Resul Karakuş, Lati̇fe Arzu Aral, Bi̇lkay Baştürk, Cemaletti̇n Aybay
Turkish Journal of Medical Sciences
Background: Hepatitis B Virus (HBV) infection is of global importance and many studies are aimed to develop more sensitive diagnostic tools and improve therapeutic options. HBV infection is diagnosed and followed-up basically by serological studies, among which the enzyme-linked immunosorbent assay (ELISA) is widely used. Aim: To develop a sensitive HBsAg ELISA for both research and diagnostic purposes. Materials and Methods: A hybridoma (clone 4D1) secreting anti-HBs IgG2a monoclonal antibody (mAb) was used for the establishment of our in-house ELISA. Tests were performed for both cell culture medium and human sera. The hepatoma cell lines of human origin PLC/PRF/5 and …
Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan
Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were …
Comparison Of The Diagnostic Value Of The Standard Tube Agglutination Test And The Elisa Igg And Igm In Patients With Brucellosis, Mustafa Ertek, Hali̇l Yazgi, Zülal Özkurt, Ahmet Ayyildiz, Mehmet Parlak
Comparison Of The Diagnostic Value Of The Standard Tube Agglutination Test And The Elisa Igg And Igm In Patients With Brucellosis, Mustafa Ertek, Hali̇l Yazgi, Zülal Özkurt, Ahmet Ayyildiz, Mehmet Parlak
Turkish Journal of Medical Sciences
Background and Aims: Brucellosis is endemic in Turkey. Since it affects many organs and the symptoms are non-specific, the diagnosis by clinical findings is difficult and may be easily missed. Many serological tests have been used for the diagnosis of human brucellosis. This study compared the diagnostic value of the Brucella standard tube agglutination test (SAT) with that of ELISA (Brucella specific IgG and IgM) tests in patients with Brucella bacteremia. Patients and Methods: Thirty-two patients with brucellosis who had positive blood and/or bone-marrow cultures for Brucella species, and 20 healthy individuals as controls were included in the study. Results: …
Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük
Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük
Turkish Journal of Veterinary & Animal Sciences
Protein bands of excretory/secretory (E/S) antigens of Dicrocoelium dendriticum were determined using SDS-PAGE and Western blotting. The E/S antigens were prepared from live adult D. dendriticum, which were obtained from slaughtered sheep at a local abattoir. In the SDS-PAGE analysis of D. dendriticum E/S antigen, 9 protein bands with molecular weights varying between 6 and 66 kDa with Coomassie staining and 14 bands between 6 and 205 kDa with silver staining were detected. Blood samples were obtained from sheep at the local abattoir. Following the examination of the organs for D. dendriticum and other helminths, serum samples were divided into …
A Comparative Study Of Detection Of Bordetella Avium Antibodies In Turkeys By Elisa, Spat, And Agid Test, Süheyla Türkyilmaz, Kenan Türkyilmaz, Osman Kaya
A Comparative Study Of Detection Of Bordetella Avium Antibodies In Turkeys By Elisa, Spat, And Agid Test, Süheyla Türkyilmaz, Kenan Türkyilmaz, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGID test. The seroprevalence was 44%, 28%, and 15% for ELISA, SPAT, and AGID test, respectively. This study revealed that ELISA was the …
Serological And Virological Investigations Of Bovine Viral Diarrhoea Virus (Bvdv) Infection In Dairy Cattle Herds In Aydın Province, Mehmet Tolga Tan, M. Taner Karaoğlu, Nural Erol, Yakup Yildirim
Serological And Virological Investigations Of Bovine Viral Diarrhoea Virus (Bvdv) Infection In Dairy Cattle Herds In Aydın Province, Mehmet Tolga Tan, M. Taner Karaoğlu, Nural Erol, Yakup Yildirim
Turkish Journal of Veterinary & Animal Sciences
A total of 288 dairy cattle from 4 different closed barns were sampled in order to investigate the presence of bovine viral diarrhoea virus (BVDV) infection. Based on the data gathered from neutralisation peroxidase-linked antibody (NPLA) tests, which were conducted to detect BVDV antibodies, all of the barns monitored previously had the infection; the rates of seropositive animals were between 44% and 100%. Overall, the results obtained from Aydın province showed that 248 of the 288 dairy cows (86%) monitored were seropositive. BVDV antigen ELISA kits (Bio-X Diagnostics- Belgium) were used to detect BVDV antigen (NS3 protein) in blood samples. …
Development Of Elisa Systems For Measurement Of Human Tumor Necrosis Factor-Alpha (Tnf-\Alpha), Ayşegül Yücel, Cemaletti̇n Aybay
Development Of Elisa Systems For Measurement Of Human Tumor Necrosis Factor-Alpha (Tnf-\Alpha), Ayşegül Yücel, Cemaletti̇n Aybay
Turkish Journal of Medical Sciences
The aim of this study was to develop a human tumor necrosis factor alpha (hTNF-\alpha) ELISA system because specific and sensitive measurement of low levels of circulating TNF-a is very important for enlightening the immunopathologial mechanisms associated with TNF-\alpha. Monoclonal antibodies 6A4c and 8A6 were produced against hTNF-\alpha and were used as the capture antibody and the tracer antibody respectively for the first hTNF-\alpha ELISA system (6A4c/biotin-8A6). Murine polyclonal IgG was used as the tracer antibody in the second hTNF-\alpha ELISA system (6A4c/biotin-polyclonal IgG). Both systems could detect both recombinant hTNF-\alpha and native hTNF-\alpha. The detection limits defined as minimal …
Seroprevalence Of Dirofilaria Immitis In Stray Dogs In İstanbul And İzmir, Taraneh Öncel, Gülay Vural
Seroprevalence Of Dirofilaria Immitis In Stray Dogs In İstanbul And İzmir, Taraneh Öncel, Gülay Vural
Turkish Journal of Veterinary & Animal Sciences
Three hundred eighty stray dogs (232 female and 148 male) from İstanbul and İzmir were tested with antigen detecting ELISA for Dirofilaria immitis infection. Blood samples were collected between November 2002 and April 2003. D. immitis infection was found in 4 dogs tested. Seroprevalence among these dogs in İstanbul was 1.52%. Seroprevalence was the highest (2.22%) in the 3-6-year-old age group, followed by 1.02% in the 1-3-year-old age group. However, there was no significant difference between these age groups (P > 0.05). No significant difference between male and female dog groups was observed in İstanbul (P > 0.05). There was no seropositivity …
Detection Of Antibodies Produced Against Ornithobacterium Rhinotracheale And Bordetella Avium By Enzyme-Linked Immunosorbent Assay In Hens And Turkeys In Aydın Province, Turkey, Süheyla Türkyilmaz, Osman Kaya
Detection Of Antibodies Produced Against Ornithobacterium Rhinotracheale And Bordetella Avium By Enzyme-Linked Immunosorbent Assay In Hens And Turkeys In Aydın Province, Turkey, Süheyla Türkyilmaz, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
Respiratory diseases are very common in poultry. Bordetella avium and Ornithobacterium rhinotracheale are among the major microorganisms responsible for these diseases. The study was conducted to investigate the seroprevalence of O. rhinotracheale and B. avium in Aydın. Two hundred seventy-six chicken and 360 turkey serum samples were collected from poultry with respiratory disease suspicion in poultry enterprises around the city and samples were then examined for the presence of B. avium and O. rhinotracheale by commercial ELISA kits. The results revealed that 66.3% of chicken and 11.1% of turkey serum samples were positive for O. rhinotracheale and 29.1% of turkey …
Sero-Prevalence Of Contagious Ecthyma In Lambs And Humans In Kars, Turkey, Hali̇l İbrahi̇m Gökce, Oktay Genç, Gürbüz Gökçe
Sero-Prevalence Of Contagious Ecthyma In Lambs And Humans In Kars, Turkey, Hali̇l İbrahi̇m Gökce, Oktay Genç, Gürbüz Gökçe
Turkish Journal of Veterinary & Animal Sciences
The sero-prevalence of contagious ecthyma (orf) in lambs and humans was studied in the Kars region of Turkey. For this purpose, serum samples were collected from 320 lambs from 5 different districts of the Kars region. Serum samples were also collected from 100 humans at risk of infection. The serum samples were tested for the presence of antibodies to orf virus by enzyme-linked immunosorbent assay (ELISA). The results showed that 52.81% and 5% of the serum samples collected from lambs and humans, respectively, were seropositive for orf virus. These results indicate that contagious ecthyma is a prevalent infection in lambs …
Seroprevalence Of Neospora Caninum In Cattle In The Province Of Şanlıurfa, Murat Sevgi̇li̇, Mehtap Gül Altaş, Oktay Keski̇n
Seroprevalence Of Neospora Caninum In Cattle In The Province Of Şanlıurfa, Murat Sevgi̇li̇, Mehtap Gül Altaş, Oktay Keski̇n
Turkish Journal of Veterinary & Animal Sciences
Blood samples were collected from 305 cows in the province of Şanlıurfa for determining the seroprevalence of Neospora caninum. Serum samples were analyzed for antibodies against N. caninum antigen using a commercially competitive inhibition (CI) ELISA. Antibodies to N. caninum were found in 23 of the 305 (7.5%) cow sera based on ELISA test results. With regard to seropositivity, no significant difference was observed among the origin, animal breed, and age (P > 0.05).
Investigations On The Presence Of Antibodies To Avian Leukosis Virus Subgroup-J (Alv-J) In Broiler Breeders And Broilers, Nuri̇ Turan, Hüseyi̇n Yilmaz
Investigations On The Presence Of Antibodies To Avian Leukosis Virus Subgroup-J (Alv-J) In Broiler Breeders And Broilers, Nuri̇ Turan, Hüseyi̇n Yilmaz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the presence of antibodies to ALV-J in broilers and broiler breeders. For this, 5 broiler units and 1 broiler breeding unit in the Marmara region were visited. Seventy 4-6 -week-old chicks from the broiler units, consisting of 14 chicks from each broiler unit, were selected. Seventeen chickens from the broiler breeding unit were also selected. The chicks from the broiler units were necropsied and all internal organs were checked for the presence of tumors. Blood sera collected from all animals were analyzed for the presence of antibodies to avian leukosis virus subgroup-J …
The Presence Of Some Anabolic Residues In Meat And Meat Products Sold In İstanbul, Bülent Nazli, Hi̇lal Çolak, Ali̇ Aydin, Hamparsun Hampikyan
The Presence Of Some Anabolic Residues In Meat And Meat Products Sold In İstanbul, Bülent Nazli, Hi̇lal Çolak, Ali̇ Aydin, Hamparsun Hampikyan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to detect some anabolic (zeranol, trenbolone, diethylstilbestrol, clenbuterol) residues in meat and meat products marketed in Istanbul. In the study, a total of 60 samples (30 fresh meat and 30 meat products) were obtained from different markets and used as test materials. The anabolic residues in the sample extracts were detected by ELISA. Initially, recovery tests were performed and then residues of one or more anabolics were analyzed in all samples. Among anabolizing residues studied, zeranol was detected in all samples (100%), trenbolone was detected in 48 samples (80%) and diethylstilbestrol was detected in …
Determination Of The Seroprevalence Of Leptospirosis In Cattle By Mat And Elisa In Hatay, Turkey, Özkan Aslantaş, Vi̇ldan Özdemi̇r
Determination Of The Seroprevalence Of Leptospirosis In Cattle By Mat And Elisa In Hatay, Turkey, Özkan Aslantaş, Vi̇ldan Özdemi̇r
Turkish Journal of Veterinary & Animal Sciences
Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars (grippotyphosa, hardjo and icterohaemorrhagie) using a microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). Forty-five (8.8%) and 72 (14%) of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively. Of the 45 MAT positive serum samples, 26 (57.7%) were positive against serovar grippotyphosa, 9 (20%) were positive against serovar hardjo, 1 (0.2%) was positive against serovar …
Seroprevalence Of Cattle Hydatidosis In Some Districts In The East Anatolian Region Of Turkey, Sami̇ Şi̇mşek, Ergün Köroğlu, Nazi̇r Dumanli, Müni̇r Aktaş, Cem Ecmel Şaki̇, Kürşat Altay, Armağan Erdem Ütük
Seroprevalence Of Cattle Hydatidosis In Some Districts In The East Anatolian Region Of Turkey, Sami̇ Şi̇mşek, Ergün Köroğlu, Nazi̇r Dumanli, Müni̇r Aktaş, Cem Ecmel Şaki̇, Kürşat Altay, Armağan Erdem Ütük
Turkish Journal of Veterinary & Animal Sciences
Hydatidosis, caused by hydatid cysts, is a widespread and hazardous disease in humans and animals worldwide. The disease is very common in Turkey, causing serious economic losses. In this study, the seroprevalence of hydatidosis was determined by enzyme-linked immunosorbent assay (ELISA) and indirect fluorescence antibody technique (IFAT). A total of 597 serum samples were collected from cattle of various breeds and ages in 8 provinces in Eastern Turkey. Partially purified cyst fluid antigen from sheep hydatid cyst fluid was used as antigen in ELISA and whole protoscolex antigens were used in IFAT. The results showed that 63.3% and 54.9% of …
Evaluation Of An Elisa For Detection Of Antibodies To Sendai Virus In Swine Sera, Raimonda Kvietkauskaite, Juzefa Acaite
Evaluation Of An Elisa For Detection Of Antibodies To Sendai Virus In Swine Sera, Raimonda Kvietkauskaite, Juzefa Acaite
Turkish Journal of Veterinary & Animal Sciences
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG serum antibodies to swine Sendai virus. To evaluate the preliminary potential of ELISA, sera from 10 healthy pigs and 34 sera from finishing pigs suffering from respiratory signs were tested. The results were compared with those obtained from the same sera by hemagglutination inhibition assay.