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Full-Text Articles in Medicine and Health Sciences
The First-Time Isolation Of Brucella Canis From Two Aborted Bitches In A Kennel In Turkey, Ahmet Murat Sayteki̇n, Mustafa Sencer Karagül, Buket Altuntaş, Gülseren Yildiz Öz, Gülnaz Meci̇toğlu, Özge Yilmaz
The First-Time Isolation Of Brucella Canis From Two Aborted Bitches In A Kennel In Turkey, Ahmet Murat Sayteki̇n, Mustafa Sencer Karagül, Buket Altuntaş, Gülseren Yildiz Öz, Gülnaz Meci̇toğlu, Özge Yilmaz
Turkish Journal of Veterinary & Animal Sciences
Brucella canis is defined as the specific agent that is responsible for canine brucellosis. In this study, B. canis was isolated and identified from the clinical materials belonging to 2 dogs kept in a breeding kennel in Turkey and brought to the researchers? clinic with abortion complaints. With this study, for the first time ever, B. canis isolation and identification was made from dogs that aborted in a kennel. This study emphasizes
First Report Of Brucella Suis Biovar 2 In Outdoor Reared Pigs(Sus Scrofa Domesticus) In Serbia, Jadranka Zutic, Djordje Cvetojevic, Ljubisa Veljovic, Oliver Radanovic, Slobodan Stanojevic, Branislav Kureljusic
First Report Of Brucella Suis Biovar 2 In Outdoor Reared Pigs(Sus Scrofa Domesticus) In Serbia, Jadranka Zutic, Djordje Cvetojevic, Ljubisa Veljovic, Oliver Radanovic, Slobodan Stanojevic, Branislav Kureljusic
Turkish Journal of Veterinary & Animal Sciences
The objective of this work was to present the first case of Brucella suis biovar 2 isolation in outdoor reared pigs in Serbia. B. suis biovar 2 has not previously been detected in Serbia, from either wild boar or outdoor reared pigs. In our case, brucellosis was discovered in the region of Srem, which geographically constitutes a part of northwestern Serbia. Outdoor reared pigs in the Srem region are part of an extensive breeding system in the field and woods. In the course of a random visit to a herd in the above-mentioned area, the animals' owner discovered two aborted …
Comparison Of The Isolation And Inhibition Abilities Ofselective Media Used For Brucella Spp. Isolation, Mustafa Sencer Karagül, Serkan İki̇z
Comparison Of The Isolation And Inhibition Abilities Ofselective Media Used For Brucella Spp. Isolation, Mustafa Sencer Karagül, Serkan İki̇z
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare and contrast the isolation ability of selective media developed for Brucella spp. isolation and their inhibition ability against contaminant microorganisms. Fifty-one field strains biotyped from abortion case samples and 25 Brucella spp. negative organ samples were used. Strain suspensions and organ suspensions were prepared separately. The turbidity of the strain suspensions was measured via spectrophotometer. The bacterial density of the strain suspensions was prepared in such a way that the suspensions would have different turbidity values. Inoculation into Farrell, Agrifood Research and Technology Center of Aragon, Jones & Morgan, and Modified Thayer …
Isolation Of Adipose Tissue-Derived Stem Cells, Asuman Özen, İrem Gül Sancak, Ahmet Ceylan, Özge Özgenç
Isolation Of Adipose Tissue-Derived Stem Cells, Asuman Özen, İrem Gül Sancak, Ahmet Ceylan, Özge Özgenç
Turkish Journal of Veterinary & Animal Sciences
Mesenchymal stem cells are being used increasingly in cell-based therapies. Adipose tissue is an important source of mesenchymal stem cells and is also routinely used in research on lipid metabolism and obesity. Their high expansion potential and the ease of isolation make these cells an attractive cell source for regenerative therapies. The objective of this review is to give detailed information about the isolation, expansion, and clinical use of these cells in veterinary medicine.
Isolation Of Streptococcus Species From The Tonsils Of Slaughtered Pigs, Arzu Funda Bağcigi̇l, Serkan İki̇z, Kemal Meti̇ner, Naci̇ye Yakut Özgür, Seyyal Ak, Ahmet Ati̇la Ilgaz
Isolation Of Streptococcus Species From The Tonsils Of Slaughtered Pigs, Arzu Funda Bağcigi̇l, Serkan İki̇z, Kemal Meti̇ner, Naci̇ye Yakut Özgür, Seyyal Ak, Ahmet Ati̇la Ilgaz
Turkish Journal of Veterinary & Animal Sciences
The palatine tonsils of 220 pigs were examined for the occurrence of Streptococcus suis and other Streptococcus species. Samples were cultured onto Colombia agar plates with 5% defibrinated horse blood and selective antibiotic supplement and were incubated at 37 °C for 24-48 h aerobically. Presumptive colonies were identified using API20 Strep (bioMérieux). S. suis was not detected in any of the 220 palatine tonsils examined. S. porcinus was the most common streptococcus recovered and was isolated from 68 (31%) pigs. From the samples, S. agalactiae was isolated from 19 (8.6%), S. equi subsp. zooepidemicus from 10 (4.5%), S. uberis from …
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Turkish Journal of Veterinary & Animal Sciences
Lung, trachea, and serum samples obtained from turkeys showing clinical signs of respiratory disease were examined for the presence of Bordetella avium infection (bordetellosis) using culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The material of this study consisted of 2 live turkeys at the age of 24 weeks with respiratory distress, nasal discharge, and cough that had been brought to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Adnan Menderes University. The results of the study show that B. avium, the causative agent of bordetellosis, was not isolated from either the lung or trachea, while both …
Serotyping Of Mannheimia Haemolytica Strains Isolated From Pneumonic Lungs Of Sheep In The Aydın Region Of Turkey, Şükrü Kirkan, Osman Kaya
Serotyping Of Mannheimia Haemolytica Strains Isolated From Pneumonic Lungs Of Sheep In The Aydın Region Of Turkey, Şükrü Kirkan, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to serotype isolates of Mannheimia haemolytica in the Aydın region of Turkey. A total of 200 lung samples showing pneumonia signs were obtained from slaughterhouses in the Aydın region. Twenty-four strains were isolated as Mannheimia haemolytica complex. Twenty-two strains were identified as Mannheimia haemolytica and 2 were as Pasteurella trehalosi for the first time in the region. The serotyping studies of strains isolated from pneumonic lung samples showed that Mannheimia haemolytica serotypes A1, A2, A6, A7, A8 and Pasteurella trehalosi serotype T4 were present in Aydın.
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR). Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed. C. perfringens was isolated from 8 (5%) of the samples. DNA samples extracted from suspected isolates grown on selective agar were amplified by PCR using a pair of primers derived from the alpha toxin gene. All of 8 suspected isolates were found to be C. perfringens by conventional methods and PCR. Isolates were …
Isolation And Serotyping Of Ornithobacterium Rhinotracheale From Poultry, Süheyla Türkyilmaz
Isolation And Serotyping Of Ornithobacterium Rhinotracheale From Poultry, Süheyla Türkyilmaz
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were the isolation of Ornithobacterium rhinotracheale from poultry and serotyping of the isolates and field sera by agar gel precipitation (AGP) test. For the isolation 257 chickens and 214 turkeys were used and for the AGP test 333 chickens and 250 turkeys were used. O. rhinotracheale was isolated from the sinus discharge of 3 broiler breeders at 37, 42 and 46 weeks of age. It was determined that 3 isolates were O. rhinotracheale serotype I having cross reactions with serotype L. Of 333 sera examined 86 were found seropositive in chickens by AGP test. It …
The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek
The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek
Turkish Journal of Veterinary & Animal Sciences
The mammalian adrenal gland in the developmental stage provides excellent system for studying the role(s) of lectins in the foetal period because the adrenal gland is composed of two separate endocrine organs which differ in embryologic origin, type of secretion, and function. There is no information on the presence of galectin-1 or galectin-1 reactive glycoconjugates in the development of the bovine adrenal gland. Therefore, the present study focused on this protein. For separation of galectin-1 dialysis, affinity chromatography and SDS-PAGE were used. To monitor the localisation of galectin-1 and its epitopes immunhistochemistry was used. A single 14-kD band comigrated with …
Isolation And Preparation Of Monoclonal Antibody To Ovineimmunoglobulin M, Hüseyi̇n Yilmaz, Aysan Tantaş, Ati̇lla Ilgaz, Kenton L. Morgan
Isolation And Preparation Of Monoclonal Antibody To Ovineimmunoglobulin M, Hüseyi̇n Yilmaz, Aysan Tantaş, Ati̇lla Ilgaz, Kenton L. Morgan
Turkish Journal of Veterinary & Animal Sciences
Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.
Isolation And Serological Characterization Of Highly Virulent Viruses Frominfectious Bursal Disease Outbreaks In Turkey, Olcay Türe, Fethi̇ye Çöven
Isolation And Serological Characterization Of Highly Virulent Viruses Frominfectious Bursal Disease Outbreaks In Turkey, Olcay Türe, Fethi̇ye Çöven
Turkish Journal of Veterinary & Animal Sciences
Infectious bursal disease (IBD) field viruses were isolated from the bursa Fabricius (BF) materials collected from the IBD infected or suspected flocks in different geographical regions of Turkey during 1992-1994. The field viruses were adapted to embryonated spesific-pathogen-free (SPF) eggs and chicken embryo fibroblast (CEF) cell cultures by serial blind passages and characterized by virus neutralization test (VN). In this study, out of 60 BF samples that were diagnosed positive for IBDV by agar gel precipitation (AGP) test, only 20 isolates adapted and grew in CEF cell cultures and showed cytopathic effect (CPE) typical to IBDV. During further characterization of …
Detection Of Listeria Species In Minced Beef, Ufuk Tansel Şi̇reli̇, İrfan Erol
Detection Of Listeria Species In Minced Beef, Ufuk Tansel Şi̇reli̇, İrfan Erol
Turkish Journal of Veterinary & Animal Sciences
In recent years there has been an increase in the number of outbreaks of human listeriosis and contaminated foods have been considered to be a potential source of infection. In this study, a total of 100 minced beef samples were obtained from different butchers and supermarkets in Ankara and examined for the presence and degree of contamination with Listeria species. The minced beef samples were collected in aseptic conditions and transported to the laboratory in cold storage. The method suggested by USDA - FSIS was used for the isolation and identification of Listeria spp. In addition, the degree of contamination …