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Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer Jan 2019

Isolation And Molecular Characterization Of Salmonella Enterica And Escherichia Coli From Poultry Samples, Ayli̇n Cesur, Saci̇de Özlem Ulutaş, Yeşi̇m Soyer

Turkish Journal of Veterinary & Animal Sciences

Innumerable foodborne pathogens including Salmonella and Escherichia coli pose a serious threat to human health and food safety. As perishable foods, poultry products are considered one of the most common sources of foodborne pathogens including Salmonella and E. coli due to transmission of drug resistance, dissemination of organisms, and cross-contamination. In our study, phenotypic and genotypic characterizations of Salmonella enterica and E. coli isolated from packaged raw chicken products were carried out. Samples belonging to different commercial brands were collected in Ankara in 2015. Among 15 out of 19 Salmonella enterica subsp. enterica strains isolated from different and/or same poultry …


In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu Jan 2019

In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu

Turkish Journal of Veterinary & Animal Sciences

The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulus-oocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium was supplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH and …


The Effects Of Supplementation Of Rumen-Protected Choline On Some Blood And Milk Metabolites In The Transition Period Of Dairy Cattle, Ni̇yazi̇ Alan, Mustafa Salman Jan 2019

The Effects Of Supplementation Of Rumen-Protected Choline On Some Blood And Milk Metabolites In The Transition Period Of Dairy Cattle, Ni̇yazi̇ Alan, Mustafa Salman

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate nonesterified fatty acid (NEFA), beta-hydroxy butyric acid (BHBA), and milk urea nitrogen (MUN) profiles for dairy cows fed with rumen-protected choline (RPC) during the transition period. A total of 30 dairy cows were allocated to one control and two experimental groups (10 animals per group) and were fed from 3 weeks before calving to 21 days in milk. The control was fed only the basal diet. Treatment groups 1 and 2 were fed a basal diet containing 60 and 120 g/head/day RPC, respectively. The differences between groups were insignificant for MUN levels …


Cointegration Analysis Of Wild And Farmed Sea Bream And Sea Bass Prices In Turkey, Mehmet Saltuk Arikan, Ahmet Cumhur Akin, Mustafa Bahadir Çevri̇mli̇, Mustafa Agah Teki̇ndal Jan 2019

Cointegration Analysis Of Wild And Farmed Sea Bream And Sea Bass Prices In Turkey, Mehmet Saltuk Arikan, Ahmet Cumhur Akin, Mustafa Bahadir Çevri̇mli̇, Mustafa Agah Teki̇ndal

Turkish Journal of Veterinary & Animal Sciences

Market integration is an indicator of the extent to which different products are interrelated, and the presence of market integration between products suggests that they are substitutes. The objective of this study was to determine the market interaction between wild and farmed products of two fish species, namely sea bream and sea bass. For this purpose, the relationship between the producer price time series of cultured sea bream, wild sea bream, cultured sea bass, and wild sea bass in the period of 2009-2017 was tested using the cointegration analysis technique. It was found that the wild sea bream and sea …


A Possible Correlation Between Diet, Serum Oxytetracycline Concentration, And Onset Of Reproductive Disturbances In Bitches: Clinical Observations And Preliminary Results, Alessandro Di Cerbo, Gianandrea Guidetti, Sergio Canello, Raffaella Cocco Jan 2019

A Possible Correlation Between Diet, Serum Oxytetracycline Concentration, And Onset Of Reproductive Disturbances In Bitches: Clinical Observations And Preliminary Results, Alessandro Di Cerbo, Gianandrea Guidetti, Sergio Canello, Raffaella Cocco

Turkish Journal of Veterinary & Animal Sciences

Based on the increase of food-related disturbances in animals and the recent findings concerning the toxicity of oxytetracycline (OTC) presence within pet food, we compared the effectiveness of two commercially available diets in 36 bitches suffering from reproductive disturbances. We ran a randomized clinical evaluation over a 90-day period. Bitches were randomly assigned to receive a control diet (CD) or a nutraceutical diet (ND) and were assessed for the presence of OTC in their sera before and at the end of the study. Sixteen of 18 bitches in the ND group went into heat within an average of 35.5 ± …


Molecular Detection And Phylogenetic Analysis Of The Honeybee (Apis Mellifera) Sacbrood Virus In Turkey, Gülnur Kalayci, Abdurrahman Anil Çağirgan, Kemal Pekmez, Buket Özkan, Murat Kaplan Jan 2019

Molecular Detection And Phylogenetic Analysis Of The Honeybee (Apis Mellifera) Sacbrood Virus In Turkey, Gülnur Kalayci, Abdurrahman Anil Çağirgan, Kemal Pekmez, Buket Özkan, Murat Kaplan

Turkish Journal of Veterinary & Animal Sciences

Sacbrood virus (SBV) is widely distributed in honeybees. It is an infectious and contagious disease, which affects both the larvae and adult stages of honeybees (Apis mellifera). This study reports the detection of sacbrood virus in Turkey in 2017. Phylogenetic analysis based on partial sequences encoding the polyprotein gene was carried out in SBV-infected bees from four different apiaries. Phylogenetic analysis revealed noteworthy results. Although European-South American, Asian, and Korean genotypes have a maximum of 5.3% variance with each other, the variation rates between them and the Turkish genotype were 10.4%, 11.4%, and 12.5%, respectively. Sequences of Turkish isolates did …


Evaluation Of Passive Transfer In Goat Kids With Brix Refractometer And Comparison With Other Semiquantitative Tests, Hasan Batmaz, Yi̇ği̇t Kaçar, Onur Topal, Zafer Meci̇toğlu, Kadi̇r Semi̇h Gümüşsoy, Feyyaz Kaya Jan 2019

Evaluation Of Passive Transfer In Goat Kids With Brix Refractometer And Comparison With Other Semiquantitative Tests, Hasan Batmaz, Yi̇ği̇t Kaçar, Onur Topal, Zafer Meci̇toğlu, Kadi̇r Semi̇h Gümüşsoy, Feyyaz Kaya

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the passive transfer (PT) status of goat kids by Brix refractometry and compare the results with other semiquantitative tests (total protein - TP, glutaraldehyde coagulation test - GCT, and gamma-glutamyltransferase - GGT) and immunoglobulin G (IgG). The study was conducted on 75 goat kids born from 47 Saanen goats. The blood samples were collected from the kids on day 0 (presuckling) and on the 1st, 2nd, and 3rd days after birth. The Brix% and TP concentrations were measured with refractometers, and GGT activity was measured using a dry chemistry system. The duration …


Impact Of Light-Emitting Diode And Compact Fluorescent Lighting Type And Cage Tier On Layers Reared In An Enriched Cage System, Part 2: Some Welfare Traits, Gülsüm Tünaydin, Bi̇lgehan Yilmaz Di̇kmen Jan 2019

Impact Of Light-Emitting Diode And Compact Fluorescent Lighting Type And Cage Tier On Layers Reared In An Enriched Cage System, Part 2: Some Welfare Traits, Gülsüm Tünaydin, Bi̇lgehan Yilmaz Di̇kmen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of lighting type [light-emitting diode (LED) and compact fluorescent (FLO) light] and cage tier on welfare traits such as feather score, body and comb wounds, bumble foot and footpad dermatitis, beak damage, keel bone deformity, finger damage, aggressive pecking behavior, and the avoidance distance test (ADT) in laying hens reared in an enriched cage system. A total of 400 layers were used to determine these traits at 25 and 45 weeks of age. A total of 80 layers were used to determine tonic immobility (TI) duration and induction number. The …


The Effect Of Laying Period On Egg Quality Traits And Chemical Composition Of Lindovskaya (Linda) Geese Reared Under Breeder Conditions, Mehmet Sari, Kadi̇r Emre Buğdayci, Aykut Asim Akbaş, Mustafa Saatci, Mustafa Numan Oğuz Jan 2019

The Effect Of Laying Period On Egg Quality Traits And Chemical Composition Of Lindovskaya (Linda) Geese Reared Under Breeder Conditions, Mehmet Sari, Kadi̇r Emre Buğdayci, Aykut Asim Akbaş, Mustafa Saatci, Mustafa Numan Oğuz

Turkish Journal of Veterinary & Animal Sciences

The study was conducted to determine the effect of laying period on egg quality traits as well as the chemical composition of Lindovskaya geese. The egg quality traits were examined by randomly taking a total of 90 eggs on the 45th ± 5, 60th ± 5, and 75th ± 5 days of the laying period from 3-year-old female Lindovskaya geese. It was determined that the effect of the laying period on external quality traits of eggs was statistically nonsignificant (P > 0.05). The effect of laying period on internal quality traits including height, diameter, and index of the yolk and albumen …


On-Farm Diagnosis Of Latent Respiratory Failure In Calves, Anton Chernitskiy, Sergey Shabunin, Tatiana Kuchmenko, Vladimir Safonov Jan 2019

On-Farm Diagnosis Of Latent Respiratory Failure In Calves, Anton Chernitskiy, Sergey Shabunin, Tatiana Kuchmenko, Vladimir Safonov

Turkish Journal of Veterinary & Animal Sciences

The goal of this study was to develop a method for the diagnosis of latent respiratory failure in calves with bovine respiratory disease (BRD) in farm conditions. The study involved 48 calves of the red-motley Holstein breed aged 14-28 days, with no previously experienced BRD, no dyspnea at rest, and a lack of anemia. Group I consisted of 24 calves with spontaneous or induced cough and WI clinical score of ≥5. Group II included 24 calves without spontaneous and induced cough and WI clinical score of ≤3. The determination of respiratory rate (RR), respiratory minute volume, and tidal volume in …


Neutralizing Antibody Titers Against Field Strains Of Bovine Viral Diarrhea Virus After Vaccination With Three Commercial Vaccines, Gi̇zem Alpay, Kadi̇r Yeşi̇lbağ Jan 2019

Neutralizing Antibody Titers Against Field Strains Of Bovine Viral Diarrhea Virus After Vaccination With Three Commercial Vaccines, Gi̇zem Alpay, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Modified killed vaccines against bovine viral diarrhea virus (BVDV) are used worldwide. In the present study, the cross-neutralization antibody responses against field strains from seven subgenotypes of BVDV-1 and one subgenotype of BVDV-2 were evaluated using sera obtained by three inactivated commercial vaccines. One vaccine contained both BVDV-1 and BVDV-2 strains, while the others contained only BVDV-1a. Three vaccine groups, each containing five calves, were vaccinated two times with 30-day intervals. The antibody titers were evaluated by virus neutralization assay. The monovalent vaccine induced the highest antibody titers. Significant levels of neutralizing antibody titers were maintained up to the last …


Effect Of Spermvital® Technology On Conception Rate In Repeat Breeder Multiparous Dairy Cows: Preliminary Results, Alper Koçyi̇ği̇t, Bariş Atalay Uslu, Sai̇t Şendağ, Eli̇sabeth Treupel, Axel Wehrend Jan 2019

Effect Of Spermvital® Technology On Conception Rate In Repeat Breeder Multiparous Dairy Cows: Preliminary Results, Alper Koçyi̇ği̇t, Bariş Atalay Uslu, Sai̇t Şendağ, Eli̇sabeth Treupel, Axel Wehrend

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effect of SV technology on conception rates in repeat breeder multiparous dairy cows. Seventy-nine multiparous Holstein cows from a private dairy farm were used in the study. These animals were cows that had failed to conceive from at least 3 regularly spaced services (repeat breeders). Estrus cycles of the cows were synchronized by 2 injections of the PG analogue, administered 11 days apart. GnRH was applied 48 h after the second injection of PG. Twenty-four h after this administration, the animals were randomly divided into 2 groups, control and SV. The …


Effects Of Supplementary Nano-Zno On In Vitro Ruminal Fermentation, Methane Release, Antioxidants, And Microbial Biomass, Hassan Riazi, Javad Rezaei, Yousef Rouzbehan Jan 2019

Effects Of Supplementary Nano-Zno On In Vitro Ruminal Fermentation, Methane Release, Antioxidants, And Microbial Biomass, Hassan Riazi, Javad Rezaei, Yousef Rouzbehan

Turkish Journal of Veterinary & Animal Sciences

The effects of nano-ZnO on in vitro ruminal fermentation, methane release, total antioxidant capacity (TAC), and microbial biomass production (MBP) were assessed using an in vitro gas production technique. Treatments included a control diet and diets containing 20, 40, or 60 mg of supplemental Zn per kg dry matter (DM) as ZnO or nano-ZnO. As a result of this study, supplementation of 20 mg of Zn as nano-ZnO, similar to ZnO, decreased methane production and protozoa enumeration but improved (P < 0.05) TAC, MBP, digestibility and truly degraded substrate (TDS). As compared with the control treatment, adding the supplementary Zn had no effect on partitioning factor, MBP efficiency, pH, and ammonia-N (P > 0.05). The diets containing 40 and 60 mg of supplementary Zn, as nano-ZnO or ZnO, had no advantage over the …


Effect Of Exercise On Some Minerals, Metabolites And Enzyme Activities In The Serum Of Trained Arabian Horses, Roya Pourmohammad, Mehrdad Mohri, Hesam Seifi, Kamran Sardari Jan 2019

Effect Of Exercise On Some Minerals, Metabolites And Enzyme Activities In The Serum Of Trained Arabian Horses, Roya Pourmohammad, Mehrdad Mohri, Hesam Seifi, Kamran Sardari

Turkish Journal of Veterinary & Animal Sciences

The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorous (P), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), zinc (Zn), sodium (Na), potassium (K), chlorine (Cl), glucose, urea, creatinine, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) were assessed in the serum of 25 Arabian horses. Blood samples were taken from the jugular vein 3 different times: before exercise, 5 h after exercise, and 18 h after exercise. Statistical analysis was performed using SAS (version 9.2; SAS Institute, Cary, NC, USA). Ca, Cl, glucose, and urea showed significant (P < 0.05) changes due to exercise. There was significant increase from before exercise to 5 h after exercise in urea concentrations while Ca and Cl concentrations significantly decreased. A significant ascending trend for Ca between 5 h and 18 h after exercise was also seen, while glucose and urea had a significantly descending trend. No significant time-based variations were observed for other variables. The results of the present study indicated that exercise could affect the value of some biochemical parameters, which could be used in future studies evaluating the health status of Arabian horses.


Abdominal Ultrasonographic Findings In Dogs With Mammary Tumors: Association With Tumor Characteristics And Survival, Daniella Matos Da Silva, Angélica Inajá Juliani, Camila Lopes Ribeiro, Jair Rodini Engracia Filho, Simone Domit Guérios, Tilde Rodrigues Froes Jan 2019

Abdominal Ultrasonographic Findings In Dogs With Mammary Tumors: Association With Tumor Characteristics And Survival, Daniella Matos Da Silva, Angélica Inajá Juliani, Camila Lopes Ribeiro, Jair Rodini Engracia Filho, Simone Domit Guérios, Tilde Rodrigues Froes

Turkish Journal of Veterinary & Animal Sciences

Abdominal ultrasound is frequently performed as part of initial staging of canine mammary tumors. The aims of this study were to describe abdominal ultrasonographic findings present at diagnosis of mammary tumors in dogs and to assess their value for associations with histopathological results and treatment outcome. The medical records of 201 female dogs diagnosed with mammary tumors that had abdominal ultrasonography performed as part of their initial clinical evaluation were reviewed. Histopathological diagnosis of malignant mammary tumors was obtained in most cases (86%; 172 cases). One hundred and fifty-seven animals (78%) had some ultrasonographic abnormality. A total of 338 ultrasonographic …


Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal Jan 2019

Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal

Turkish Journal of Veterinary & Animal Sciences

In this study, we established the components of the buffalo spermatogonial stem cell (SSC) niche in prenatal (38.5-280 days of gestation), neonatal, prepubertal, and adult testes histochemically and immunohistochemically. Immunohistochemically, the changes in vimentin expression were evaluated. During prenatal life, the niche of primordial germ cells was formed by the surrounding mesenchymal cells and fibroblasts. The basement membrane (BM) was not organized at this stage. At 7.6 cm crown-rump length, testicular cords with a thin BM were seen. At the late gestational period, testicular cords with well-organized BM were seen. At the neonatal stage, the testicular cords were lined with …


The Effect Of Anesthetic (2-Phenoxyethanol) Application On Some Biochemical And Hematological Parameters In Russian Sturgeon (Acipenser Gueldenstaedtii) And Siberian Sturgeon (Acipenser Baerii) During Transport, Selçuk Duman Jan 2019

The Effect Of Anesthetic (2-Phenoxyethanol) Application On Some Biochemical And Hematological Parameters In Russian Sturgeon (Acipenser Gueldenstaedtii) And Siberian Sturgeon (Acipenser Baerii) During Transport, Selçuk Duman

Turkish Journal of Veterinary & Animal Sciences

Experiments were carried out to determine the effect of 2-phenoxyethanol to reduce stress response during the transportation of Russian and Siberian sturgeons. In transportation experiment 1 (TE$_{1}$), Siberian sturgeons (687.2 ± 17.41 g) were stocked in tanks and transported for 90 min (stocking density = 65 ± 0.71 kg/$m^{3}$). Before transport, 0.5 mg/L 2-phenoxyethanol was applied to the first group (TE$_{1-A}$, n = 285) but not to the second group (TE$_{1-B}$, n = 287). In transportation experiment 2 (TE2), Russian sturgeons (1873 ± 159 g) were divided into two groups and stocked in tanks as they have a density of …


Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost Jan 2019

Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost

Turkish Journal of Veterinary & Animal Sciences

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into …


The Comparing Of Homogenization Methods For Oxidative And Antioxidative Analyses Of Sperm, Ömer Varişli, Numan Akyol Jan 2019

The Comparing Of Homogenization Methods For Oxidative And Antioxidative Analyses Of Sperm, Ömer Varişli, Numan Akyol

Turkish Journal of Veterinary & Animal Sciences

Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differences can affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semen of four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods. Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, free sulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods before oxidative and antioxidative status analyses significantly …