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Full-Text Articles in Medicine and Health Sciences
Demonstration Of Mutations Conferring Resistance To Lamivudine In Liver Tissue Of Chronic Hepatitis B Patients Under Lamivudine Therapy, İsmai̇l Yaşar Avci, Can Polat Eyi̇gün, Emi̇ne Günal, Ayhan Kubar, Hanefi̇ Cem Gül, Ömer Coşkun, Hakan Erdem, Levent Görenek, Bülent Ahmet Beşi̇rbelli̇oğlu
Demonstration Of Mutations Conferring Resistance To Lamivudine In Liver Tissue Of Chronic Hepatitis B Patients Under Lamivudine Therapy, İsmai̇l Yaşar Avci, Can Polat Eyi̇gün, Emi̇ne Günal, Ayhan Kubar, Hanefi̇ Cem Gül, Ömer Coşkun, Hakan Erdem, Levent Görenek, Bülent Ahmet Beşi̇rbelli̇oğlu
Turkish Journal of Medical Sciences
To demonstrate the genotypic lamivudine resistance in liver tissue of patients with negative serum hepatitis B virus (HBV) DNA under long-term lamivudine therapy. Materials and methods: Fourteen patients (10 patients with elevated alanine aminotransferase and 4 patients under long-term lamivudine therapy for severe liver disease) with undetected serum HBV DNA were included in the study. A liver biopsy was performed for each patient, and HBV DNA extracted from liver tissue was analyzed with TaqMan real-time polymerase chain reaction. Results: Mutations conferring lamivudine resistance were shown for all 14 patients in their liver tissue-extracted HBV DNA. One patient had rtM204V, 4 …
Jak2 V617f Mutation In Iranian Patients With Myeloproliferative Neoplasms: Clinical And Laboratory Findings, Behzad Poopak, Majid Farshdousti Hagh, Najmaldin Saki, Fazel Elahi, Hamid Rezvani, Gelareh Khosravipour, Mohammad Ali Jahangirpour, Shirin Bolouri, Tolou Golkar, Parviz Falah
Jak2 V617f Mutation In Iranian Patients With Myeloproliferative Neoplasms: Clinical And Laboratory Findings, Behzad Poopak, Majid Farshdousti Hagh, Najmaldin Saki, Fazel Elahi, Hamid Rezvani, Gelareh Khosravipour, Mohammad Ali Jahangirpour, Shirin Bolouri, Tolou Golkar, Parviz Falah
Turkish Journal of Medical Sciences
The JAK2 V617F mutation has been described as a frequent genetic event among a majority of patients with polycythemia vera, essential thrombocythemia, and myelofibrosis. In this research, we evaluated the prevalence of the JAK2 mutation and its clinical and laboratory correlation in patients with myeloproliferative neoplasms. Materials and methods: A total of 615 patients with suspected myeloproliferative neoplasms (MPNs) were analyzed for the JAK2 V617F mutation. After DNA extraction, detection of the mutation was done using allele-specific PCR. Positive samples were subsequently analyzed with PCR-RFLP by the restriction endonuclease BsaXI. The patients were also analyzed for hematological indices. Results: Of …