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A Modified Method For The Determination Of Plasma Total Homocysteine By High Performance Liquid Chromatography, Serhat Tokgöz, Cumhur Bi̇lgi̇, Erdi̇nç Çakir, M. Kemal Erbi̇l, Türker Kutluay
A Modified Method For The Determination Of Plasma Total Homocysteine By High Performance Liquid Chromatography, Serhat Tokgöz, Cumhur Bi̇lgi̇, Erdi̇nç Çakir, M. Kemal Erbi̇l, Türker Kutluay
Turkish Journal of Medical Sciences
We have modified a high-performance liquid chromatography (HPLC) procedure for homocysteine assay using 7-fluoro-2, 1, 3-benzoxadiazole-4-sulfonamide (ABD-F) pre-column derivatization. In this study, the thiol compounds were liberated from plasma proteins by reduction with 2-mercaptoethanol (BME) and derivatized with a thiol-specific fluoregenic marker, ABD-F. The derivatives were separated by reversed-phase HPLC on a Hypersil C 18 column (4.6 x 150 mm; 5 µ) using isocratic elution with 0.1 M phosphate buffer pH 6.0 containing 8% methanol and fluorescence detection. Excitation and emission wavelengths were 386 and 516 nm. The peak of homocysteine was eluted at 413 ± 25 s. Mean within-day …
Fasting And Post-Methionine Load Plasma Homocysteine Levels In Patients With Angiographically Defined Coronary Artery Disease, Mustafa Özkan, M. Kemal Erbi̇l, Münzer Al Rashed, Emi̇n Özgür Akgül, Tuncer Çayci, Cumhur Bi̇lgi̇, Gürkan Meti̇nyurt, Şeri̇f Akman, Türker Kutluay
Fasting And Post-Methionine Load Plasma Homocysteine Levels In Patients With Angiographically Defined Coronary Artery Disease, Mustafa Özkan, M. Kemal Erbi̇l, Münzer Al Rashed, Emi̇n Özgür Akgül, Tuncer Çayci, Cumhur Bi̇lgi̇, Gürkan Meti̇nyurt, Şeri̇f Akman, Türker Kutluay
Turkish Journal of Medical Sciences
Elevated plasma homocysteine concentration either in fasting state or after methionine loading is an independent risk factor for vascular disease. The aim of this study was to evaluate the relationship between fasting total homocysteine (tHcy) and post-methionine load tHcy in healthy control subjects and in patients with angiographically defined coronary artery disease (CAD). Methionine loading has been used to investigate impaired methionine metabolism, especially of the trans-sulphuration pathway. We investigated the effect of methionine excess on total plasma homocysteine in 65 control subjects (44 men and 21 women) who attended a polyclinic of Gülhane Military Medical Academy (GMMA) in Ankara. …