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Full-Text Articles in Medicine and Health Sciences
Measurement Of Urine Albumin Levels With A Monoclonal Antibody Based In-House Elisa, Cemaletti̇n Aybay, Resul Karakuş
Measurement Of Urine Albumin Levels With A Monoclonal Antibody Based In-House Elisa, Cemaletti̇n Aybay, Resul Karakuş
Turkish Journal of Medical Sciences
Microalbuminuria is considered to be an early indicator of diabetic nephropathy. We have previously reported the establishment of A-HAlb/98 hybridoma secreting monoclonal antibody that specifically recognizes human serum albumin (HSA). In this report, an in-house enzyme-linked immunosorbent assay (ELISA) was developed using this antibody for the measurement of urinary albumin (UA) and UA was examined in 24 different samples. A-HAlb/98 was used as the capture antibody by immobilizing onto microtitre plates in ELISA format. HSA-specific polyclonal rabbit immunoglobulin was labelled with biotin and then used as the tracer antibody. The dynamic assay range of ELISA for albumin was established to …
Development Of A Diagnostic And Screening Elisa System For Measuring Tetanus Antitoxoid Levels, Cemaletti̇n Aybay, Resul Karakuş, Ahmet Gökhan Gündoğdu
Development Of A Diagnostic And Screening Elisa System For Measuring Tetanus Antitoxoid Levels, Cemaletti̇n Aybay, Resul Karakuş, Ahmet Gökhan Gündoğdu
Turkish Journal of Medical Sciences
Tetanus is a vaccine-preventable disease of global importance. When acquired it has a high mortality rate. Screenings of special subpopulations such as women of childbearing age provide important clues about tetanus vaccination coverage, and tools for mass protective measures, e.g, protective measures for neonatal tetanus. In addition to its reliability and sensitivity, the main advantage of a mass-screening tool should be its cost-effectiveness. We aimed to develop a cost-effective and sensitive enzyme-linked immunosorbent assay (ELISA) that can be used for diagnostic research as well as mass screening purposes for antitetanus antibodies. Our in-house antitetanus ELISA was validated and tested for …