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The Effect Of Diphtheria Toxin On Nitric Oxideinduction From Raw264.7 Murine Macrophages, Cemalettin Aybay, Gülnur Tarhan, Turgut İmi̇r, Başak Kayhan
The Effect Of Diphtheria Toxin On Nitric Oxideinduction From Raw264.7 Murine Macrophages, Cemalettin Aybay, Gülnur Tarhan, Turgut İmi̇r, Başak Kayhan
Turkish Journal of Medical Sciences
In this study the effect of diphtheria toxin (DT) on nitric oxide (NO) production from RAW 264.7 murine macrophages was investigated. Griess reagent was used to determine NO production by measuring nitrite levels in the culture supernatants. Corynebacterium diphtheriae G12/6 strain-produced DT (Limes flocculation activity and immunodiffusion assays were positive) demonstrated a dose-dependent effect on RAW 264.7 macrophages to induce NO production. L-NAME, an L-arginine analogue, inhibited NO production from DT-stimulated RAW 264.7 macrophages. At the given concentrations of DT, lipopolysaccharide (LPS or endotoxin)-induced NO production from RAW 264.7 macrophages was also inhibited. RAW 264.7 macrophage cells, incubated with various …
Plasma Antioxidant Status And Nitrate Levels Inpatients With Hypertension Andcoronary Heart Disease, Bülent Sözmen, Cahit Kazaz, Dilek Taskiran, Leyla Aslan, Akan Akyol, Eser Yıldırım Sözmen
Plasma Antioxidant Status And Nitrate Levels Inpatients With Hypertension Andcoronary Heart Disease, Bülent Sözmen, Cahit Kazaz, Dilek Taskiran, Leyla Aslan, Akan Akyol, Eser Yıldırım Sözmen
Turkish Journal of Medical Sciences
In this study, we investigated first; the possible involvement of nitric oxide and antioxidant enzyme level in the pathogenesis of essential hypertension and second we investigated serum nitrite-nitrate and antioxidant enzyme activities and lipid profiles (total, HDL and LDL cholesterol, trygliceride) to determine the relationship between these parameters in atherosclerosis. 13 patients with coronary heart disease, 18 patients with essential hypertension and 16 age-matched health subjects were taken into this study. Plasma nitrite, nitrate levels were measured by Griess reaction, erythrocyter superoxide dismutase and catalase activities were determined by the methods of Misra&Fridovich and Aebi, respectively. Serum lipid analysis were …