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The Effects Of Phenoxybenzamine On Tyrosinehydroxylase (Th) And Th Mrna Level In Adrenalmedulla Of Sprague Dawley Rats, Muhittin Yürekli̇
The Effects Of Phenoxybenzamine On Tyrosinehydroxylase (Th) And Th Mrna Level In Adrenalmedulla Of Sprague Dawley Rats, Muhittin Yürekli̇
Turkish Journal of Medical Sciences
The effects of anti-hipertensive phenoxybenzamine were in-vestigated on tyrosine hydroxylase (TH) enzyme activity and TH mRNA levels. In the present study 5 months male sprague dawley (SD) rats were used. Phenoxybenzamine was injected i.p as 20 mg/kg which prepared in the 0.9 % NaCl and 5% ethanole. TH activity was measured by detecting of formation of 3 H 2 O as a formation of dopa from 3 H-tyrosine by radioisotope technique total adrenal RNA was isolated and hybridized wit 32 P labeled cDNA. TH mRNA was assayed by densitometric scanning of the autoradiograms using a densitometer. TH activity and TH …