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Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the …
Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep
Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep
The Thai Journal of Veterinary Medicine
Fas ligand (FasL) and its receptor (Fas) are tumor necrosis factor (TNF) members which are involved in theimmune privileged organs, such as the cornea, testis and placenta, by triggering apoptosis in various cell types. Theappearance of a Fas-FasL system might specify the immune privileged status of the buffalo oviduct wherespermatozoa avoid eradication by female immune cells. The objective of this study was to scrutinize the FasL and Fasproteins by immunohistochemistry, FasL mRNA by RT-PCR and the apoptotic degrees by TUNEL analysis in theuterotubal junction (UTJ), isthmus, ampulla and infundibulum of swamp buffalo oviducts at follicular (n=5) and midluteal(n=5) phases. The …