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Full-Text Articles in Medicine and Health Sciences
E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai
E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The main purpose of the present study was to investigate the localization of epithelial cadherin (E-cadherin) in the oviduct and uterine horn of swamp buffalo at the follicular and mid-luteal phases. E-cadherin is a transmembrane glycoprotein which plays a vital role in the mechanism of the maintenance epithelial architecture, cell growth and proliferation, cell to cell adhesion including cell to epithelium adhesion. Female reproductive tracts of swamp buffaloes (n=30) were collected from local abattoirs and categorized into follicular (n=15) and mid-luteal (n=15) phases. Tissue samples from the oviducts and uterine horns were regularly handled according to histological and immunohistochemical techniques. …
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the …
Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep
Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep
The Thai Journal of Veterinary Medicine
Fas ligand (FasL) and its receptor (Fas) are tumor necrosis factor (TNF) members which are involved in theimmune privileged organs, such as the cornea, testis and placenta, by triggering apoptosis in various cell types. Theappearance of a Fas-FasL system might specify the immune privileged status of the buffalo oviduct wherespermatozoa avoid eradication by female immune cells. The objective of this study was to scrutinize the FasL and Fasproteins by immunohistochemistry, FasL mRNA by RT-PCR and the apoptotic degrees by TUNEL analysis in theuterotubal junction (UTJ), isthmus, ampulla and infundibulum of swamp buffalo oviducts at follicular (n=5) and midluteal(n=5) phases. The …
Immune Cell Population In Oviducts In Relation To Ovarian And Progesterone Status Of Anoestrous And Repeat Breeding Gilts, Paisan Tienthai, Sakaoporn Prachantasena, Supassama Chaiyawong, Tharnath Nanthirudjanar, Padet Tummaruk
Immune Cell Population In Oviducts In Relation To Ovarian And Progesterone Status Of Anoestrous And Repeat Breeding Gilts, Paisan Tienthai, Sakaoporn Prachantasena, Supassama Chaiyawong, Tharnath Nanthirudjanar, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The aim of the present study is to determine the distribution of immune cells within the endosalpinx related to the ovarian function in gilts culled due to repeat breeding and anoestrus. A total of 30 reproductive organs from culling crossbred Landrace x Yorkshire gilts were collected from commercial swine herds in Thailand and categorized into 3 groups, i.e. anoestrus, repeat breeding and non-involved in reproductive disorder gilts (control group). The ovaries and oviducts were separated from the reproductive tracts and macroscopically investigated. The oviducts were divided into the utero-tubal junction (UTJ), the isthmus, the ampulla and the infundibulum. The gilts …
Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong
Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong
The Thai Journal of Veterinary Medicine
The present study aimed to examine the general morphology of Thai swamp buffalo’s oviducts by light microscopy (LM) and scanning electron microscopy (SEM) as well as the expression of estrogen receptor alpha (ERα) and progesterone receptor isoform B (PRB) by immunohistochemical technique at the prepubertal stage. The samples composed of the uterotubal junction (UTJ), isthmus, ampulla and infundibulum of prepubertal Thai swamp buffalo oviducts (n=6) were collected from a local slaughterhouse. The results showed that the epithelium of prepubertal Thai swamp buffalo oviducts was composed of ciliated and secretory cells but the feature of nuclear/cytoplasmic protrusions of secretory cells was …
Expression Of The Progesterone Receptor In The Thai Swamp Buffalo Oviduct At The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu
Expression Of The Progesterone Receptor In The Thai Swamp Buffalo Oviduct At The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The objective of this study was to investigate the presence and distribution of the progesterone receptor (PR) in the Thai swamp buffalo oviduct at the follicular (n=10) and luteal phases (n=12). Reproductive tracts from mature female Thai swamp buffaloes were collected from a local slaughterhouse. Both ovaries were examined for the phase of estrous cycle and the oviducts were separated into the uterotubal junction (UTJ), the isthmus, the ampulla and the infundibulum. Tissue samples were subjected to immunohistochemical staining to assess the localization of PR in the epithelium, subepithelial connective tissue and smooth muscle. A positive nuclear immunolabeling of PR …
Estrogen Receptor Alpha Localization In Thai Swamp Buffalo Oviduct During The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu
Estrogen Receptor Alpha Localization In Thai Swamp Buffalo Oviduct During The Follicular And Luteal Phases, Paisan Tienthai, Kriengyot Sajjarengpong, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
Estrogens are known to be an important modulator for regulation of the reproductive function in female mammals. The specific receptor of estrogens in the buffalo reproductive tract, particularly in the oviduct, has not been explored yet in the expression pattern. This study was, therefore, undertaken to investigate the localization of the estrogen receptor alpha (ERα) in the oviduct of Thai swamp buffalo at the follicular and late luteal phases of the estrous cycle. The oviducts were collected from swamp buffalo genital tracts at the local abattoir and separated into the uterotubal junction (UTJ), isthmus, ampulla and infundibulum. Tissue distribution of …
Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk
Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the morphological changes of the oviduct from 30 culling replacement gilts and 6 control sows. Blood samples and female genital organs were taken immediately after slaughter. Post-mortem examinations were made on the genital organs and oviductal samples, i.e. utero-tubal junction (UTJ), isthmus and ampulla, were collected and fixed for histological analysis. Observations indicated that the reasons for culling the gilts were 'no estrus' (68%) and 'repeat breeding' (32%). The greatest pathological changes (67%) were found in repeat breeding gilts, whereas 52% of no estrous gilts were at prepubertal phase. The female hormonal …
An Immunolohistochemical Study Of Anti-Apoptotic Bcl-2 In The Pig’S Oviduct During The Oestrous Cycle, Paisan Tienthai
An Immunolohistochemical Study Of Anti-Apoptotic Bcl-2 In The Pig’S Oviduct During The Oestrous Cycle, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The purpose of this study was to determine the localization of Bcl-2 protein in porcine oviducts. Anti-human Bcl-2 monoclonal antibody was used to localize Bcl-2 protein immunohistochemically in the uterotubal junction (UTJ), isthmus and ampulla of pre-ovulatory (n=10) and post-ovulatory (n=10) sows. The Bcl-2 protein was detected in quantity in the intracytoplasm of the epithelial cells, some types of leukocytes in the epithelial layer and in the subepithelial connective tissue, in fibroblasts, as well as smooth muscle cells of the UTJ, isthmus and ampulla in the pre-ovulatory period. In contrast, the expression of Bcl-2 was lower in the oviduct tissues …
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
The Thai Journal of Veterinary Medicine
During sperm transport in the female pig, a proportion of the spermatozoa is arrested at the uterotubal junction and the isthmus, where an oviductal sperm reservoir is built up. The sperm reservoir maintains sperm viability and controls the release of fertilizing spermatozoa so that only a small subpopulation reaches the site of fertilization, thus diminishing the risk of polyspermy. In vitro research has focused on sperm binding as the main mechanism of sperm storage, sperm release and modulation of capacitation, but little attention has been paid to the sperm reservoir fluid, its composition and the control of capacitation in vivo. …
Fas Ligand Expression In The Porcine Oviduct, Paisan Tienthai, Yumi Hoshino
Fas Ligand Expression In The Porcine Oviduct, Paisan Tienthai, Yumi Hoshino
The Thai Journal of Veterinary Medicine
The expression of Fas/Fas Ligand (FasL) is thought to be involved in the immune privileged status of the testis, cornea and the placenta. The objective of this study was to see whether FasL mRNA was expressed in the epithelium of the porcine oviduct, where spermatozoa, oocytes and developing embryos avoid elimination by the female immune system during the estrous cycle. The distribution of Fas/FasL might provide information about immune privileged sites in the pig oviduct. The gene expression of FasL was detected in the uterotubal junction (UTJ), isthmus and ampulla of the pig oviductal epithelium, during both pre- and post-ovulatory …
The Function Of The Oviduct In Relation To Sperm Transport And Capacitation In Swine, Paisan Tienthai
The Function Of The Oviduct In Relation To Sperm Transport And Capacitation In Swine, Paisan Tienthai
The Thai Journal of Veterinary Medicine
In the pig, sperm transport is regulated, to facilitate normal fertilization of the ovulated oocytes, by the oviduct which contains a particular segment, the sperm reservoir. The main function of the sperm reservoir is to ensure that stored spermatozoa have maintained viability and fertilizing capacity. The process of sperm capacitation is delayed in the sperm reservoir, during the lengthy pre-ovulatory estrus by interactions between the spermatozoa, the oviduct epithelium and secretion products. Sperm capacitation seems to be facilitated by the tubal fluid in the upper segment of the oviduct releasing sperm from the reservoir in relation to the time of …