Medicine and Health Sciences Commons^™

Condylar Angulations As Related To Submental-Vertex Radiographs, Jady L. Chiakowsky

Loma Linda University Electronic Theses, Dissertations & Projects

Pretreatment submental-vertex radiographs were obtained for 637 adolescent patients (307 males, 330 females) who had presented themselves for orthodontic treatment. The purpose of this study was to: (1) determine the relationship between condylar angulations and depth of cut with the variables: age, sex, and race, and (2) establish normative values for the categories studied. The results indicated that no significant differences existed between the condylar angulations for the variables measured. However, significant differences did exist between the depth of cut for the variables measured and there was significant interaction between age and race with respect to the depth of cut. …

Characteristics Of Habitual Dysfunctional Behaviors, Carole Ryan Serena

Loma Linda University Electronic Theses, Dissertations & Projects

The suggestion that habitual dysfunctional behavior disorders (HDBs) have a common underlying cluster of addictive characteristics and dynamics is new. A broad research base which would support this theory has not kept current with clinical In order successes. In order to address the need for relevant research, an anonymous cross sectional study was designed to identify, describe and compare variables associated with 15 habitual dysfunctional behaviors (HDBs) in a sample (N =176) from a broad spectrum of socioeconomic groups.

People reporting two different HDBs (alcohol and food-related behaviors) were compared to those not reporting any HDBs on levels of four …

Modulation Of Rat Hepatic S9-Dependent Mutagenesis, Dna Binding, And Metabolism Of Aflatoxin B1 And Benzo[A]Pyrene By Four Chinese Medicinal Herbs, Brian Yuen Yau Wong

Loma Linda University Electronic Theses, Dissertations & Projects

Extracts of the herbs Oldenlandia diffusa (OD) and Scutellaria barbata (SB) have been used in traditional Chinese medicine for treating liver, lung and rectal tumors while extracts of Astragalus membranaceus (AM) and Ligustrum lucidum (LL) are often used as adjuncts in cancer therapy. In this study, we determined the effects of aqueous extracts of these four herbs on aflatoxin B₁ (AFB₁) benzo[a]pyrene (BaP), benzo[a]pyrene 7,8-dihydrodiol (BaP 7,8-DHD) and benzo[a]pyrene 7,8-dihydrodiol-9,10-epoxide (BPDE)-induced mutagenesis, mediated by enzymes in rat hepatic 9000 x g supernatant (S9), in Salmonella typhimurium TA100. We also studied the effects of these four herbs on …

The Periodontal Intraosseous Defect Following Root Conditioning With Citric Acid And Wound Closure Including A Ptfe Membrane : An Experimental Study, Betty Glaser Kersten

Loma Linda University Electronic Theses, Dissertations & Projects

Treatment of the intrabony or intraosseous periodontal defect has rendered much attention over the last few decades. Numerous approaches have been advocated; however, overall comparisons between treatments do not exist. This review summarizes and discusses outcomes of therapeutic modalities in the intrabony periodontal lesion from controlled clinical studies. The modalities reviewed include scaling and root planning, gingival flap surgery, gingival flap surgery including wound closure with interdental denudation, root-biomodification, placement of autologous, allogenic, or xenogeneic bone grafts, placement of alloplastic grafts, and placement of tissue guiding barrier membranes. In addition histologic observations of healing in the intrabony periodontal lesion are …

The Expression And Regulation Of The Hsdk Genes Of Escherichia Coli K-12, Ainu Parkash

Loma Linda University Electronic Theses, Dissertations & Projects

The DNA restriction and modification (R-M) genes in bacteria play a defensive role in the organism, offering protection to the host from invaders such as bacteriophages. The expression of these genes in E. coli K-12 had not previously been studied due to the complexity of the EcoK R-M enzymes. The use of lacZ gene fusion techniques allowed studies of the expression of the hsdK genes for the first time. Operon fusions of Mu(/ac) to the hsdK genes show that the two hsdK promoters, p _res and p _mod, are weak (50 Miller units) and that …

Food Handling Practices Among Independent-Living Elderly, Judith Dianne Mcdonough

Loma Linda University Electronic Theses, Dissertations & Projects

Food has long been recognized as a vehicle of disease transmission; there are more reported illness associated with the consumption of food than all other environmental factors combined. The handling and storage of food are potentially dangerous and demanding chores for those who may have the sensory losses associated with advancing age, fragile health, and limited resources.

Group-administered questionnaires on the knowledge, attitudes, and practices of food handling were given to 276 seniors, aged 55 to 90, from ten mobile home parks randomly selected from San Bernardino and Riverside counties. As an indicator of possible foodborne illness, the questionnaire asked …

Dysfunctional Attitudes, Life Stress And Coping Processes, Deborah Desalliers

Loma Linda University Electronic Theses, Dissertations & Projects

The findings of recent investigations earmarked chronic daily stressors as being highly related to stress levels. In addition, researchers are promoting a more integrative model of stress. The stress response is no longer thought of in strictly linear terms, i.e. an event causes stress. Rather, cognitive factors, existing within the human mind itself, strongly influence our coping processes and the chronic stress we experience.

Canonical correlation analysis was used to study how dysfunctional attitudes, a negative cognitive factor, were related to individuals' daily hassles and coping processes. An increase in dysfunctional attitudes was found to correspond to an increase in …

The Effect Of Ethanol On Bone Histomorphology, Rebecca L. Rodocker

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of ethanol on bone histomorphology was studied using an isocaloric liquid pair feeding system to administer equal amounts of essential vitamins and nutrients to control and ethanol animals.

A bone remodeling sequence was induced in the lower right mandible by removal of the upper right molars. The bone remodeling unit, consisting mainly of the osteoclast and osteoblast, could then be studied microscopically to note any changes brought about by the administration of ethanol.

Although the method of ethanol administration was successful, the induction of unintended bone remodeling via the removal of a solid diet made analysis of the …

The Role Of T Cells In The Pathogenesis Of Periapical Lesions, John B. Wallstrom

Loma Linda University Electronic Theses, Dissertations & Projects

The pulps of mandibular molars of 15 athymic and 15 conventional rats were surgically exposed and left open to their oral flora. Each group was divided into 3 subgroups of 5 animals each. The rats were sacrificed after their pulps were exposed for 2, 4, or 8 weeks. After fixing, decalcifying, and embedding, the specimens were sectioned and stained with H and E. They were then examined under a microscopic grid and quantified by percentages of surface areas of bone, connective tissue, bone marrow, intrabony spaces, periapical lesions, and numbers of osteoclasts. This was done using a DataVoice computerized data …

Effect Of Age On The Structure Of Meissner Corpuscles In Forepad Digital Pads Of Mice, Roger C. Mathewson

Loma Linda University Electronic Theses, Dissertations & Projects

Meissner corpuscles in forepaw digital pads of albino mice were examined by light and electron microscopy to determine structural age-changes. After 1.5 months of age, no intraepidermal nerve fibers were seen extending from corpuscles. From young (1.5-6 months) to middle age (9-15 months), corpuscles became larger and more complex, gaining more horizontally arranged terminals, associated lamellae and connective tissue. At old age (18-26 months), corpuscles became small and lobulated, appearing disorganized. There was no loosening of the corpuscle-epidermal interface. An increase in collagenous connective tissue and basal lamina duplication occurred with advancing age. Ultrastructural age-changes consisted of disorganization of the …

Histomorphometric Analysis Of Primate Periodontal Tissues Following Corticotomy In Orthodontics, Wuiteng Koh

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the prevalence of specific cell types and structures at the active tooth movement site in corticotomized and non-corticotomized specimens obtained from a previous study, and to consider the hypothesis that corticotomy facilitated orthodontics is efficacious due to an inflammatory response rather than tooth-bone block movement. Specimens from 3 monkeys, each of which had a treatment and control quadrant in each arch, were sectioned, mounted on glass slides, and stained with Giemsa, haematoxylin, and Safranin stains. A histomorphometric analysis was performed with DataVoice™, a voice activated computer system which allowed for digitization of …

The Fracture Strength Of Ceramic Brackets : An Update And Comparison, Christian P. Manley

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to compare the fracture strength of ceramic brackets which are currently on the market with ceramic brackets tested in a previous study at Loma Linda. Fracture strengths of standard edgewise ceramic brackets vs. pre-torqued ceramic brackets and bicuspid ceramic brackets vs. central incisor ceramic brackets were also compared.

A total of 160 Polycrystalline brackets, 8 categories in groups if 20, were tested for fracture strength. Each bracket was tested using the same testing model that was used in the previous study at Loma Linda. The brackets were tested by torquing a wire within the …

A Flow Cytometric Analysis Of Immunocyte Populations In Peripheral Blood And Pleural Effusions Of Patients With Cancer, Mandy L. Bohn

Loma Linda University Electronic Theses, Dissertations & Projects

Two-color flow cytometric analysis was performed on paired samples of peripheral blood (PB) and pleural effusions (PE) of patients with metastatic malignancies. The purpose of these analyses was to test the hypothesis that there are significant differences in the distribution of immunocyte populations in the PE compared to that of the PB.

The majority of the pleural fluid immunocytes were CD3+ cells. The ratio of CD4+ to CD8+ cells was higher in the PE compared to PB. Levels of CD8+, CD19+ and CD14+ cells were not significantly different in the PE compared to the levels in the PB. CD16+CD56+ cells …

The Accuracy Of Sonographic Imaging (Dolphintm Digigraph) And Standard Lateral Cephalometric Radiographs : A Comparative Study, Michael D. Teichman

Loma Linda University Electronic Theses, Dissertations & Projects

Radiation due to x-ray exposure is a concern, and the development of the Dolphin™ DigiGraph represents an attempt to limit this exposure. The purpose of this paper was to compare measurements (using Ricketts analysis) obtained from conventional radiographic cephalometric tracings with those obtained by computerized digitizing with the DigiGraph. Forty-six patients from the Loma Linda University School of Dentistry graduate orthodontic clinic were digitized, and the results were compared to the x-ray tracings of these patients. The results showed that seven of the twenty-one variables examined exhibited a statistical similarity between the two methods (DigiGraph and x-ray tracing). An examination …

The Use Of Corrected Occlusal X-Ray For Determination Of Ald : A Comparative Study, Merlin D. Ekvall

Loma Linda University Electronic Theses, Dissertations & Projects

In this study arch length discrepancy or ALD was studied in 50 untreated patients. The measurements obtained from Hilgers' corrected occlusal x-ray using his Occlusal Paralleling Instrument or O.P.I, were compared with those derived by four conventional methods, - "Eye balling," digital caliper determination, conventional mandibular x-ray, and the Rocky Mountain Data Systems computer analysis (31 cases). When using the OPI, results give a higher negative ALD as it takes into consideration the space required to level a curve of Spee.

Measurements were made by the author and 3 faculty members using the above methods. Statistical analysis of the means …

The Impact Of Variations In Buccolingual Thickness Of Maxillary Anterior Dentition On First Order Arch Form Adjustments, Craig A. Andreiko

Loma Linda University Electronic Theses, Dissertations & Projects

Pre-adjusted bracket systems offer the clinician the convenience of working with both fewer in number and smaller magnitude adjustments in the archwire. The purpose of this study was to examine the normal variations in the thickness of the six maxillary anterior teeth and how this relates to anticipated first order bends in the archwire. A second purpose was to examine these same teeth for bilateral symmetry.

Thirty casts from the Loma Linda University completed treatment files were examined with respect to variations in the thicknesses of the crowns at the level of the archwire plane. From these, the mean difference …

The Effectiveness Of Minimal Pair Therapy For S-Cluster Reduction, Julie Anne Mcdow

Loma Linda University Electronic Theses, Dissertations & Projects

This descriptive study compared minimal pair therapy to a modeling imitative method of phonological remediation. The s-cluster was targeted for remediation. Four phonologically impaired children, ages 3-2 to 3-11, served as subjects. In the minimal pair procedure, minimal pairs were used to contrast the meaning of correctly produced target words and incorrectly produced target words. In the non-minimal pair approach, correct productions were modeled and cued for the subjects to imitate/produce. Generalization to untrained words was insufficient to assess the effectiveness of the two procedures used. Other measures made during the course of the study produced some interesting findings. Imitative …

Cephalometric Analysis Of Soft Tissue Profile Change Associated With Orthodontics And Orthognathic Surgery : A Long-Term Study, Douglas M. Brown

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the effect of combined orthodontic and orthognathic surgical treatment upon the soft tissue profile, and to determine the correlation between soft tissue response and the following variables: type of surgery, genioplasty, lip thickness, method of surgical site closure, interlabial distance and eversion of the lower lip. Preoperative and long-term postoperative lateral cephalograms were traced for 327 patients (231 females, 96 males) who had been treated with orthognathic surgery (108 Le Fort I, 109 mandibular sagittal split, 110 combined Le Fort I and mandibular sagittal split surgery). The results indicated that strong correlations …

Catecholamines And The Regulation Of Uterine Contractions In The Pregnant Rhesus Macaque, Cahleen Mae Mcnutt

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to test the hypothesis that: 1) maternal and/or amniotic fluid catecholamines or prostaglandins display nocturnal activity rhythms similar to those of the myometrial contractility in the pregnant rhesus macaque and, 2) catecholamines stimulate prostaglandin production by fetal membranes. Paired maternal arterial blood and amniotic fluid samples were collected at 0900 h (AM) and 2100 h (PM) from chronically catheterized rhesus macaques until delivery and analyzed for prostaglandin metabolites (PGFM and PGEM-II) and catecholamines (norepinephrine, epinephrine, and dopamine). Uterine activity data was continuously recorded. A rhythm in uterine contractility was observed, with peak activity between 1900 and …

Molecular Mechanism Of The Stimulation Of Alkaline Phosphatase Activity In Human Bone Cells By 1,25(Oh)2 D3, Eru Kyeyune-Nyombi

Loma Linda University Electronic Theses, Dissertations & Projects

To facilitate this study an in vitro human model system was established that exhibited many aspects of normal osteoblasts. The human osteosarcoma cell line (TE85 cells) expressed a skeletal alkaline phosphatase activity (an accepted bone cell differentiation marker) which was stimulated by 1,25(OH)₂D₃ (a potent differentiating agent), under serum-free conditions in a dose-dependent, time-dependent, and cell density-dependent manner. Cytochemical analysis of the stimulation of ALP activity by 1,25(OH)₂D₃ showed that 1,25(OH)₂D₃ increased the number of TE85 cells that expressed detectable ALP activity, suggesting that 1,25(OH)₂D₃ promoted the process …

Topographic Development Of The Corticocollicular Projection In The Neonatal Rat, F. Allen Richburg Ii

Loma Linda University Electronic Theses, Dissertations & Projects

The density and distribution of labeled cortical cells was examined following discrete deposits of retrograde tracers into the superior colliculus (SC) of neonatal and adult rats to study the topographic order of SC afferents from the cortex during the initial phase of axon ingrowth. Small deposits of red and green latex microspheres (0.02-0.05ul) were placed into the SC of rats ranging in age from birth to adulthood using glass micropipettes (tip diameter 25-35um). The animals, following a 48hr survival period, were deeply anesthetized and perfused with normal saline followed by a 10% formal-saline solution. The brains were sectioned coronally at …

Nutrient Intake And Urine And Serum Calcium Response To A Low Calcium Diet And Repletion, Nancy Roberts

Loma Linda University Electronic Theses, Dissertations & Projects

A high percentage of American women do not consume the Recommended Dietary Allowance for calcium. Marginal intake of calcium (Ca) in the years when peak bone mass is being formed may have a detrimental impact on bone later in life. This study looked at the effect of low Ca intake and other dietary components on serum and urine values of Ca as well as the response to repletion.

Seventeen free-living females, age 21 to 30 years, were placed on a low Ca diet (/day) for 22 days after a baseline of intake >800 mg/day for at least 6 weeks. Calcibind …

Concentration Of Leukotrienes B4 And C4 In Human Periradicular Lesions, Elisabetta Cotti

Loma Linda University Electronic Theses, Dissertations & Projects

The 5-lipoxygenase metabolites of arachidonic acid such as leukotrienes (LT) B₄ and C₄, are potent mediators of inflammation. The purpose of this investigation was to determine the concentrations of LTB₄ and LTC₄ in human periradicular lesions. Specimens from asymptomatic and symptomatic patients were obtained and immediately frozen in liquid nitrogen. Periradicular tissues from unerupted third molars as well as chronically inflamed gingival tissues were also obtained, frozen and were used as negative and positive controls respectively. The concentrations of LTB₄ and LTC₄ were determined by the reverse phase high pressure liquid chromatography. Representative …

Assessment Of Maternal Substance Abuse And Neonatal Exposure, Carol Jean Dauenhauer Giese

Loma Linda University Electronic Theses, Dissertations & Projects

Infants born to adolescent and young adult mothers who abuse substances, are at high risk for chemical dependency and its sequelae. There is a need to identify such infants and their mothers so that intervention will be available to them. The purpose of this study was to develop a systematic approach to evaluate mothers and infants for potential drug use and improve the quality of the nurses decisions.

This research project involves two sub-projects. In Part A, a Substance Abuse Assessment Tool (SAAT) was developed. The hospital nurses used the SAAT March 1, 1990, through April 12, 1990. The incidence …

The Effects Of Moderate Exercise Training On Nutrient Intake In Mildly Obese Women, Leann Onasch

Loma Linda University Electronic Theses, Dissertations & Projects

The relationship between moderate exercise training (five 45 min sessions/week, brisk walking at 62+2% V0_2max for 15 weeks) and changes in nutrient intake was investigated in a group of 36 sedentary, mildly obese women, conducted using a two (exercise, EX and nonexercise, NEX groups) x three (baseline, six-, 15-week testing sessions) factorial design, with data analyzed using repeated measures ANOVA. The pattern of change over time between groups for kilocalorie intake tended to be different between groups [F(2,68)=2.50,p=0.089] with the EX group experiencing a significant decrease versus baseline by 15 weeks. Significant group x time interactions were found for …

Standardization Of Oral Cloze As A Language Assessment Device For Bilingual Hispanic Children, Shannon Ventuleth

Loma Linda University Electronic Theses, Dissertations & Projects

This present study was initiated to determine the feasibility of oral cloze procedures as a language screening for bilingual Hispanic students. If such a procedure were normed, and proven to be both reliable and valid, the resultant assessment device would have potential screening tool as a for determining the presence of a language disorder in the bilingual Hispanic child aged 8 through 10 years.

A review of current tests available, and a review of the literature, revealed few language tests designed specifically for and normed on bilingual Hispanic children. Tests available for bilingual Hispanic children over 7 years of age …

Proto-Oncogene Regulation By Growth Factors In Bone Cells, Harold Lyndon Merriman

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies have shown that bone cells in culture produce a number of growth factors that are important in bone regulation. Chick and mouse primary calvarial cultures consist of a mixture of cell types that make it difficult to interpret the results from these model systems. In contrast, MC3T3-E1 cells are a clonal mouse osteoblast-like cell line. Since MC3T3-E1 cells consist of a single population of calvarial cells, they make an ideal system in which to study the autocrine effects of bone growth factors.

Based on this work, MC3T3-E1 cells are now known to produce IGF-I, TGF-beta and IGF-II in …

Development Of Catalytic Rnas As Potential Anti-Hiv-1 Agents, Pairoj Sae Chang

Loma Linda University Electronic Theses, Dissertations & Projects

RNA molecules with enzymatic activity (ribozymes) targeted to the genome of HIV-1 were developed and demonstrated to mediate site-specific cleavage of the HIV-1 RNA in vitro and to inhibit viral proliferation intracellularly. Two classes of ribozymes that incorporate a "hammerhead" catalytic center were utilized, eotide elements in the viral sequence to provide half of the The first class used nuclcatalytic center. A complementary strand harboring the remaining active site nucleotides was supplied in trans and directed the site-specific cleavage of the HIV-1 RNA target. A second type of ribozyme incorporated the entire catalytic center, minus the site of cleavage, within …

The Long Term Stability Of The Dental Arches Following Rapid Palatal Expansion, Robert C. Stockdale

Loma Linda University Electronic Theses, Dissertations & Projects

The rapid palatal expander is an appliance which has been used to laterally expand the maxillary dental arch, with subsequent mandibular dental arch expansion.

The purpose of this study was to evaluate and quantify long term post treatment changes in the length and width of both dental arches following rapid palatal expansion.

Plaster models from eleven patients treated with rapid palatal expansion were obtained seven or more years after treatment. The dental arches were intact anterior to the first molars and no retreatment had taken place.

Maxillary and mandibular canine, premolar and molar arch width, arch length, arch circumference, and …

A Modified Nance Unilateral Molar Distalizer, Tracy J. Reiner

Loma Linda University Electronic Theses, Dissertations & Projects

This clinical study evaluated a modified Nance appliance designed by the author to distalize molars unilaterally for Class II correction. This fixed appliance did not rely upon extraoral traction or intraoral elastics. Twelve unilateral Class II patients aged 13 to 17 were treated nonextraction with the appliance. Pretreatment and posttreatment photographs were taken of the palatal soft tissue and did not reveal any untoward changes as a result of the Nance acrylic button. All molars were corrected to an Angle's Class I relationship. Molar correction was evaluated visually and molar distalization was measured using an acrylic jig fitted to the …