Medicine and Health Sciences Commons^™

Development Of Catalytic Rnas As Potential Anti-Hiv-1 Agents, Pairoj Sae Chang

Loma Linda University Electronic Theses, Dissertations & Projects

RNA molecules with enzymatic activity (ribozymes) targeted to the genome of HIV-1 were developed and demonstrated to mediate site-specific cleavage of the HIV-1 RNA in vitro and to inhibit viral proliferation intracellularly. Two classes of ribozymes that incorporate a "hammerhead" catalytic center were utilized, eotide elements in the viral sequence to provide half of the The first class used nuclcatalytic center. A complementary strand harboring the remaining active site nucleotides was supplied in trans and directed the site-specific cleavage of the HIV-1 RNA target. A second type of ribozyme incorporated the entire catalytic center, minus the site of cleavage, within …

Proto-Oncogene Regulation By Growth Factors In Bone Cells, Harold Lyndon Merriman

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies have shown that bone cells in culture produce a number of growth factors that are important in bone regulation. Chick and mouse primary calvarial cultures consist of a mixture of cell types that make it difficult to interpret the results from these model systems. In contrast, MC3T3-E1 cells are a clonal mouse osteoblast-like cell line. Since MC3T3-E1 cells consist of a single population of calvarial cells, they make an ideal system in which to study the autocrine effects of bone growth factors.

Based on this work, MC3T3-E1 cells are now known to produce IGF-I, TGF-beta and IGF-II in …

Standardization Of Oral Cloze As A Language Assessment Device For Bilingual Hispanic Children, Shannon Ventuleth

Loma Linda University Electronic Theses, Dissertations & Projects

This present study was initiated to determine the feasibility of oral cloze procedures as a language screening for bilingual Hispanic students. If such a procedure were normed, and proven to be both reliable and valid, the resultant assessment device would have potential screening tool as a for determining the presence of a language disorder in the bilingual Hispanic child aged 8 through 10 years.

A review of current tests available, and a review of the literature, revealed few language tests designed specifically for and normed on bilingual Hispanic children. Tests available for bilingual Hispanic children over 7 years of age …

The Long Term Stability Of The Dental Arches Following Rapid Palatal Expansion, Robert C. Stockdale

Loma Linda University Electronic Theses, Dissertations & Projects

The rapid palatal expander is an appliance which has been used to laterally expand the maxillary dental arch, with subsequent mandibular dental arch expansion.

The purpose of this study was to evaluate and quantify long term post treatment changes in the length and width of both dental arches following rapid palatal expansion.

Plaster models from eleven patients treated with rapid palatal expansion were obtained seven or more years after treatment. The dental arches were intact anterior to the first molars and no retreatment had taken place.

Maxillary and mandibular canine, premolar and molar arch width, arch length, arch circumference, and …

Correlation Of Condylar Angulation To Occlusion, Facial Type, Age, And Sex, Perry A. Stevenson

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the correlation between the condylar angulation and the following variables: occlusion, facial type, depth of cut, overbite, overjet, interincisal angle, age, and sex. Pretreatment lateral cephalograms, submental vertex radiographs and study models were obtained for 114 patients (66 females, 48 males) with a mean age of 16 who were considered to have normal, asymptomatic temporomandibular joints. The results indicated that no significant correlation existed between the condylar angulation and the variables measured. Therefore, there is no method of predicting the condylar angulation by occlusion characteristics, facial type, age, or sex.

Maternal Immunomodulation Of Neonatal Alloantigen Response, Leh Chang

Loma Linda University Electronic Theses, Dissertations & Projects

Remarkable success has been achieved in the transplantation of allogeneic cardiac grafts into newborn infants at Loma Linda University Medical Center. The superior graft survival rate documented in these patients has not correlated with the degree of immunosuppression rendered, or the selection of genetically matched donors. However, the clinical success has correlated with the age of the recipient at the time of receiving a transplant. Patients receiving an allograft within the first few weeks of life are unique in that they seem to accept the alloantigens of their cardiac graft while responding aggressively to antigens in their environment. These observations …

Active Treatment Time And Incisor Root Resorption Of Malocclusions Treated With Corticotomy Facilitated Orthodontics Vs. Non-Corticotomy Treatment, Douglas F. Tetz

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the variables of treatment time and root resorption comparing subjects treated with corticotomy-facilitated orthodontics and controls treated by conventional methods. Twelve adult corticotomy subjects were utilized for the treatment time variable and seven of these subjects were utilized for the root resorption variable. These subjects were matched with non-corticotomized adult controls with regards to malocclusion, facial type, overbite, overjet and arch length deficiency. Average treatment time was found to be decreased by an average of 50% for those treated by corticotomy-facilitated orthodontics compared to non-corticotomy facilitated orthodontic care. Root resorption was significantly …

Molar And Incisor Changes With Wilson "Rapid Molar Distalization", D. Dween Muse

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Evaluation Of Headgear Safety : A Comparative Study, William J. Pritchard

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the relative safety of headgear safety straps. Samples from five different safety headgear straps simulated orthopedic and orthodontic use and data were collected from a series of pulls on an Instron machine. The straps were compared by the distance the face bow traveled before the safety mechanism released, the force built into the face bow at safety release and the force required to remove the inner bow out of the molar tube. The conclusions are as follows: (1) the distance a face bow can travel from the molar tube and still be …

Effect Of Glucose Administration On Hamster Liver S9-Mediated Mutagenesis, Metabolism And Dna-Binding Of Benzo[A]Pyrene And Aflatoxin B1, Daniel C. Rubano

Loma Linda University Electronic Theses, Dissertations & Projects

Hamster liver S9 prepared from control animals and animals given 30% glucose in drinking water 48 h before time of sacrifice was used in studies of benzo[a]pyrene (BaP) and aflatoxin (AFB₁)-induced mutagenesis, metabolism of BaP and AFB₁, and metabolite binding to calf thymus DNA. BaP-induced mutagenesis in Salmonella typhimurium TA100 was reduced 38.5% while AFB₁-induced mutagenesis was increased 36% by S9 from glucose-treated hamsters. The reduction of [³H]BaP metabolite binding to calf thymus DNA in incubations with S9 from glucose-treated hamsters correlated with a decrease in unknown BaP metabolite-deoxyribonucleoside adducts isolated by …

Characterization Of Hsd::Mudx128 Operon Fusion Mutant Of Escherichia Coli K-12, Marjorie Ann T. Reyno

Loma Linda University Electronic Theses, Dissertations & Projects

The study of the regulation of gene expression in complex genes can be facilitated by the use of operon fusions which place a well characterized lacZ gene under the control of a promoter of interest. An hsd::MudX128 operon fusion mutant of E. coli K-12 isolated by Prakash (1986) was observed to have a high β-galactosidase activity (1000 units), ten to twenty times higher than other hsd::MudX mutants. This β-galactosidase activity was suppressed to a very low level (25 units) by the introduction of an F’ plasmid, F'101, which carries the 98 min to 2 min region of the …

A Modified Nance Unilateral Molar Distalizer, Tracy J. Reiner

Loma Linda University Electronic Theses, Dissertations & Projects

This clinical study evaluated a modified Nance appliance designed by the author to distalize molars unilaterally for Class II correction. This fixed appliance did not rely upon extraoral traction or intraoral elastics. Twelve unilateral Class II patients aged 13 to 17 were treated nonextraction with the appliance. Pretreatment and posttreatment photographs were taken of the palatal soft tissue and did not reveal any untoward changes as a result of the Nance acrylic button. All molars were corrected to an Angle's Class I relationship. Molar correction was evaluated visually and molar distalization was measured using an acrylic jig fitted to the …

The Relationship Between Depression, Dyspnea, And Physical Activity In Patients With Severe Chronic Obstructive Pulmonary Disease, Carolyn J. Calhoun

Loma Linda University Electronic Theses, Dissertations & Projects

This correlational descriptive study investigated the relationship between depression, degree of dyspnea, and performance of daily activities in male and female patients with severe chronic obstructive pulmonary disease (COPD). The problem was to determine (a) the influence of depression on the degree of dyspnea experienced and performance of daily activities, and (b) the relationship between the degree of dyspnea experienced and the performance of daily activities by male and female patients with severe COPD.

This study contained a convenience sample of 49 patients who met the research criteria (38 males, 10 females, and one patient unidentified as to gender). All …

Effect Of Disulfiram On The Urinary Excretion Of 5-Hydroxytryptophol And 5-Hydroxyindoleacetic Acid In Subjects Consuming Serotonin Rich Foods, Amy G. Tordecilla

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of disulfiram and/or serotonin rich foods (SRF) on the urinary excretion of 5-hydroxytryptophol (5-HTOL) and 5-hydroxyindoleacetic acid (5-HIAA) in normal male subjects was investigated. Urine collections were obtained on 1) regular serotonin free diet, 2) SRF, 3) serotonin free diet with disulfiram, and 4) SRF with disulfiram. Urinary excretion of 5-HTOL in ug/gm creatinine during disulfiram administration was 112.0 ± 24.1. Five HTOL (ug/gm creatinine) with disulfiram and SRF increased to 1532 ± 352.0 (p < 0.001). Five-HIAA (mg/g creatinine) before and after SRF was 3.4 ± .44 and 33.1 ± 6.1 respectively, p < 0.001. With disulfiram and SRF the excretion of was 24.2 ± 3.2 mg/g creatinine which was significantly greater than when on the regular diet (p < 0.001) than the excretion on SRF alone (p < 0.05)

Key words: Disulfiram, serotonin, 5-hydroxytryptophol, 5-hydroxyindoleacetic acid, urinary excretion, high performance liquid chromatography, alcoholism

The Stability Of Mandibular Bilateral Sagittal Split Osteotomy Advancements Using Rigid Skeletal Fixation, Michael E. Fox

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

The Concentration Of Prostaglandin E2 In Periradicular Lesions, Steve N. Mcnicholas

Loma Linda University Electronic Theses, Dissertations & Projects

Prostaglandins (PG) have been implicated in pathogenesis of periapical lesions. The purpose of this study was to determine the concentrations of PGE₂ in human periapical lesions. Periapical specimens from patients with clinical signs and symptoms of chronic and acute apical periodontitis were obtained and immediately frozen in liquid nitrogen. Periradicular tissues from unerupted third molars were frozen and used as controls. The concentration of PGE₂ was determined by radioimmunoassay. Low levels of PGE₂ were found in the control tissues as compared to those detected in chronic and acute lesions. A significantly higher level of PGE₂ was …

The Effect Of An Anti-Osteoclastic Agent And A Collagen Sponge On Root Resorption Following Regenerative Periodontal Surgery In The Dog, Rudolf Hahn

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies indicate that root resorption is a frequent sequela to significant amounts of periodontal repair following experimental regenerative periodontal surgical procedures in animal models. To study if root resorption can be reduced or prevented, periodontal repair was evaluated in large horizontal circumferential defects in the dog in the absence of influence from the oral environment. Diphosphonate (Cl₂ MDP) applied topically to the root surfaces prior to wound closure as well as the use of a collagen sponge membrane (Helistat®) draped over the root surfaces and a control with no additional treatment after defect creation were the modalities employed. …

Effect Of Photoperiod On Developmental Morphology And Enolase Isoenzyme Immunohistochemistry In Rat And Djungarian Hamster Superficial Pineal Glands, Chalmer D. Mcclure

Loma Linda University Electronic Theses, Dissertations & Projects

The best understood functional activity of the pineal gland is its diurnal production of melatonin in response to environmental lighting cues. Several enzymes of the melatonin pathway respond to daily photoperiod changes, for example hydroxyindole-O-methyltransferase (HIOMT) and serotonin N-acetyltransferase (SNAT). Increased levels of the glycolytic enzyme neuron-specific enolase (NSE) are thought to reflect increased physiological demands placed on neurons and neuroendocrine tissues. Homodimer non-neuronal enolase isoenzyme (NNE) is immunolocalized to cells, and the hybrid enolase (consisting of subunits from NSE and NNE) has been seen in cerebellar stellate and basket cells. Although not rate limiting, concentrations of both NSE and …

Misfit Between Motivational Style And Type Of Job Demand As A Factor In Developing Occupational Stress, Arlene Gray Blix

Loma Linda University Electronic Theses, Dissertations & Projects

Occupational stress is costly in terms of human suffering and impaired organizational effectiveness. Occupational stress involves the interface between the individual worker and the work environment. The purpose of the study was to analyze the fit between motivational style and the type of job demands as a contributing factor in developing occupational stress symptoms. A literature review of occupational stress models was conducted. The six models compared and contrasted included the Person-Environment fit model (French, Rodgers, & Cobb, 1974), Integrative Transactional Model (Schuler, 1982), Organizational Stress Models (Ivancevich & Matteson, 1987; Parker & DeCotiis, 1983), Facet Analysis Model (Beehr & …

A Statistical Evaluation Of Class Iii Indicators In A Caucasian Population, Javier Canut

Loma Linda University Electronic Theses, Dissertations & Projects

In order to evaluate the significance of seven Class III indicators (molar relation, cranial deflection, ramus position, porion location, Xi to Pt Vertical, Xi to SNa Vertical and Xi-S-Na angle) seventy cases with a Class III malocclusion and excessive facial depth were selected from the files of the Loma Linda University Orthodontic Department, Loma Linda University Oral Surgery Department and Fundacion Jimenez Diaz Hospital Orthodontic Department (Madrid, Spain), and compared to a control group of seventy non-Class III cases.

Means and standard deviations of all variables were calculated and compared between the sample and control groups. Significant differences were determined …

Co-Cr Comparison Between Untreated & Treated Cl Ii Div 1 Cases, James W. Davis

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this retrospective study was to compare the COCR differences in pretreatment and posttreatment CI II Div I patients. Similar sample groups were chosen from patients of the graduate orthodontic clinic at Loma Linda University School of Dentistry.

Each patient's models were mounted on a Sam II articulator using a face bow transfer and a centric relation check bite. The COCR discrepancies in the anterior-posterior, superior-inferior, and side-shift dimensions were determined with the Mandibular Position Indicator.

Using t-test analyses, statistically significant differences were found between the pretreatment and posttreatment groups, with the posttreatment sample exhibiting a smaller CO-CR …

Load Deformation Test Of Metal Brackets : A Comparative Study, Luke K. Choi

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine the effect material and design (slot torque degree and wing type) had on the force and stress to permanently deform metal brackets.

Fourteen different types of metal brackets were tested and categorized into three categories. The three categories were: raw material composition, slot torque degree, and wing type. There were 5 types of raw materials (310SS, 316L, 303SE, 3038, and 17-4PH), 3 types of slot torque degree (0 degree, 7 degree, and 12 degree), and 4 types of wing design (mini twin, single, regular twin, and modified twin). All brackets were tested …

In Vitro Evaluation Of Cancer Patient Immune Responses Following Infusion Of Radiolabeled Murine Monoclonal Antibody, Kelly A. King

Loma Linda University Electronic Theses, Dissertations & Projects

Several monoclonal antibody (MAb) types derived from mice have been developed specifically for the use of targeting human tumors. The specificity of these murine MAb’s for their respective tumor antigens is very high making them potentially good immunotherapeutic agents for cancer treatment and diagnosis. A limiting factor in using these murine MAb’s in patients is the development of human anti-mouse antibody (HAMA). The presence of HAMA may reduce the effectiveness of MAb for tumor targeting. If patient sensitization could be quickly detected following the initial infusion of MAb, then future infusion could be altered or terminated to prevent further sensitization. …

Religious Commitment As A Predictor Of Health Behavior And Health Status In A Selected Population, Donald George King

Loma Linda University Electronic Theses, Dissertations & Projects

Religious beliefs and behaviors are multidimensional and they profoundly determine the ways we respond to health and illness, suffering and death. Researchers have designed numerous scales to measure different dimensions of religious commitment, beliefs and behaviors. A thorough review of the literature has shown general relationships between religion and health, but no researcher has developed an instrument measuring dimensions of religious commitment specifically relevant to health behaviors.

In this study, scale items were developed to measure three dimensions of religious beliefs thought by Kenneth Vaux (1976) to be related to health behavior--purity of life, peace in existence, and belief in …

Process Of Health Care Decision Making Among Khmer Immigrants, Barbara Frye

Loma Linda University Electronic Theses, Dissertations & Projects

This qualitative study examined the congruence between health beliefs and behavior as reported by 30 Cambodian (Khmer) refugee women in Southern California. Utilizing in-home interviewing in the Khmer language, data was gathered on 226 illness episodes occurring among 157 family members over an eight-month span. Informants reported a strong maternal role in health care decision making with all ages of children. Adult decision making demonstrated an individualistic pattern. Causes of illness were attributed primarily to humeral imbalances and illness avoidance behavior reflected these beliefs. Treatment was a blend of scientific and traditional medicine. Health care was accessed in settings of …

Needs Of Family Members Of Patients In The Critical Care Unit And The Nurse's Perception Of Those Needs, Nicolle C. Sobieszyk

Loma Linda University Electronic Theses, Dissertations & Projects

The family is greatly affected when one member is admitted to a critical care unit. Critical care nurses are in an ideal position to help families cope with the crisis of a critical illness. Those who are knowledgeable of family member needs will provide more compassionate and effective nursing care to both the patient and family.

This study investigated the needs of family members of patients in the critical care unit and compared them to the critical care nurse's perception of those needs. The sample consisted of 55 critical care nurses and 51 family members of critically ill patients from …

Enhancement Of Intratumor Antibody Distribution With A Bifunctional Antibody Delivery System And With Hyperthermia, Karen Lynn King

Loma Linda University Electronic Theses, Dissertations & Projects

The intratumor distribution of a new monoclonal antibody-hapten-based delivery system was examined. From two marine antibodies, Hybritech, Inc. has developed a bifunctional antibody (BPA) with specificities against CEA (ZCE-025 or GEM 231) and against one of two derivatives of [¹¹¹In]-benzyl-EDTA (CHA 255), EOTUBE or BLEDTA IV, to form ZCE/CHA or CEM/CHA. BFA accumulates in CEA-expressing tissue and clears from non-antigenic tissues prior to administration of an [¹¹¹In]-hapten.

Using five groups of nude mice bearing human colon tumor xenografts (T380), we evaluated different carriers of [¹¹¹In]-EOTUBE to prelocalized ZCE/CHA. We administered all antibodies and haptens …

Feasibility Of An Extraoral Method For Predicting Manibular Incisor Relapse, Mark J. Saylor

Loma Linda University Electronic Theses, Dissertations & Projects

The author undertook to develop an extraoral model for predicting mandibular incisor relapse similar to the model described by Harvey and Sheldon Peck. Whereas the Peck and Peck method involves intraoral measurements, the method described in this paper relied on the taking of measurements from the patient's pre-treatment plaster models. Two hundred and sixty teeth were measured both orally and on plaster casts, and several statistical tests were performed to isolate any differences between the intraoral and extraoral methods. The principal difference was identified as the faciolingual measurement of the incisor, due to the height of contour often being subgingival …

Regulation Of Epidermal Growth Factor Receptor Synthesis By Retinoic Acid And Dexamethasone, Kerby C. Oberg

Loma Linda University Electronic Theses, Dissertations & Projects

Fetal rat lung (FRL) cells incubated in the presence of retinoic acid or dexamethasone exhibit altered ¹²⁵I-epidermal growth factor (EOF) binding capacity. Retinoic acid enhances ¹²⁵I-EGF binding 3-fold following 24 hours of 37° incubation. In contrast, FRL cells incubated in the presence of dexamethasone display a 2-fold reduction in ¹²⁵I-EGF binding capacity. The change in binding capacity, induced by either hormone, was due to alterations in the average number of receptors per cell as determined by Scatchard analysis of ¹²⁵I-EGF binding. In the absence of retinoic acid or dexamethasone, FRL cells were calculated to have approximately …

Anthropometric Parameters In Public School Students And Seventh-Day Adventist Vegetarian And Meat-Eating Students, Joan Sabaté-Casellas

Loma Linda University Electronic Theses, Dissertations & Projects

Concerns have been expressed for the ability of some vegetarian diets to support normal growth of children. Animal protein or meat intake showed a positive relationship to attained height in children from developing countries. In this study, the relationship between meat intake/vegetarian status and physical growth was investigated in children living in California.

Height and weight were collected on 2,272 Caucasian children aged 6-18 years attending Seventh-day Adventist (SDA) or public schools who participated in a two year longitudinal study. Age-, sex-, and school-specific mean heights and weights were at or above the national reference values. Sex-specific, age-adjusted regression analysis …