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Full-Text Articles in Medicine and Health Sciences
Structural Analysis Of Gelsolin Using Synchrotron Protein Footprinting, Janna G. Kiselar, Mark R. Chance
Structural Analysis Of Gelsolin Using Synchrotron Protein Footprinting, Janna G. Kiselar, Mark R. Chance
Faculty Scholarship
Protein footprinting provides detailed structural information on protein structure in solution by directly identifying accessible and hydroxyl radical-reactive side chain residues. Radiolytic generation of hydroxyl radicals using millisecond pulses of a synchrotron "white" beam results in the formation of stable side chain oxidation products, which can be digested with proteases for mass spectrometry (MS) analysis. Liquid chromatography-coupled MS and tandem MS methods allow for the quantitation of the ratio of modified and unmodified peptides and identify the specific side chain probes that are oxidized, respectively. The ability to monitor the changes in accessibility of multiple side chain probes by monitoring …
Achieving The Right Balance In Oversight Of Physician Opioid Prescribing For Pain: The Role Of State Medical Boards, Diane E. Hoffmann, Anita J. Tarzian
Achieving The Right Balance In Oversight Of Physician Opioid Prescribing For Pain: The Role Of State Medical Boards, Diane E. Hoffmann, Anita J. Tarzian
Faculty Scholarship
No abstract provided.
Okazaki Fragment Maturation In Yeast: Ii. Cooperation Between The Polymerase And 3′-5′-Exonuclease Activities Of Pol Δ In The Creation Of A Ligatable Nick, Yong Hwan Jin, L. Rao Ayyagari, Michael A. Resnick, Dmitry A. Gordenin, Peter M.J. Burgers
Okazaki Fragment Maturation In Yeast: Ii. Cooperation Between The Polymerase And 3′-5′-Exonuclease Activities Of Pol Δ In The Creation Of A Ligatable Nick, Yong Hwan Jin, L. Rao Ayyagari, Michael A. Resnick, Dmitry A. Gordenin, Peter M.J. Burgers
Faculty Scholarship
To address the different functions of Pol δ and FEN1 (Rad27) in Okazaki fragment maturation, exonuclease-deficient polymerase Pol δ-01 and Pol δ-5DV (corresponding to alleles pol3–01-(D321A, E323A) and pol3–5DV-(D520V), respectively) were purified and characterized in this process. In the presence of the replication clamp PCNA, both wild-type and exo− Pol δ carried out strand displacement synthesis with similar rates; however, initiation of strand displacement synthesis was much more efficient with Pol δ-exo−. When Pol δ-exo− encountered a downstream primer, it paused with 3–5 nucleotides of the primer displaced, whereas the wild type carried out precise gap filling. Consequently, in the …
Federalism And The Future Of Health Care Reform, Richard Briffault, Sherry Glied
Federalism And The Future Of Health Care Reform, Richard Briffault, Sherry Glied
Faculty Scholarship
An important theme in the ongoing health care reform debate is federalism. During the battle over the Clinton Health Plan in 1993–94, the question of which level of government — federal or state — should take the leading role in health policy was almost as contentious as the particular proposals for extending access to quality health care and controlling health care costs. With the failure in 1994 to achieve comprehensive legislation at the national level, many policymakers and commentators gave fresh attention to the states as potential agents for health care reform.