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Four-Week Induction Of Type 2 Diabetes Mellitus In Rats By Streptozotocin And High-Fat Diet, Wirin Bowonsomsarit, Weerapat Anegkamol, Natthaya Chuaypen, Maroot Kaewwongse, Piyaratana Tosukhowong, Phisit Prapunwattana, Thasinas Dissayabutra

Chulalongkorn Medical Journal

Background: Current rodent models for diabetes mellitus type 2 could be divided into genetic-engineered and environmental-induced. Since genetic-modified rodents are difficult to acquire and costly for Thai researchers, we tried to develop a protocol for food-induced diabetic rats. Objectives: To develop a convenient and economical type 2 diabetic mellitus rats with a short course (4 - week) of induction for research purpose. Methods: Eight-week-old male Wistar rats were divided into two groups: control group and streptozotocin with high-fat diet (STZ-HFD) group (n = 4 each). The control group received normal chow (c.p.082 diet with 20.0% energy from fat). For the …