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Anti-Inflammatory Effect Of Alpha Lipoic Acid Loaded Calcium Citrate Nanoparticle On Human Keratinocyte Hacat Cells, Boonyaras Bukkavesa, Siwaporn Nilyai, Kanidta Sooklert, Sasin Thamakaison, Amornpun Sereemaspun
Anti-Inflammatory Effect Of Alpha Lipoic Acid Loaded Calcium Citrate Nanoparticle On Human Keratinocyte Hacat Cells, Boonyaras Bukkavesa, Siwaporn Nilyai, Kanidta Sooklert, Sasin Thamakaison, Amornpun Sereemaspun
Chulalongkorn Medical Journal
Background: Alpha lipoic acid (LA) is potent anti-oxidant and anti-inflammation natural compound. To enhance the efficacy and stability of LA, LA-loaded calcium citrate nanoparticles were developed.Objective: To investigate the anti-inflammatory effects of LA-loaded calcium citrate nanoparticles (LA-NPs) in HaCaT keratinocytes.Methods: HaCaT cells were exposed under two inflammation stimuli conditions, lipopolysaccharide (LPS) and Pb(NO3)2, and then were treated with or without LA and LA-NPs for 24 h. Cell viability and pattern of cell death were evaluated. In addition, the expression levels of inflammatory cytokines including IL-1β, IL-6, and TNF-α as well as inflammatory mediator COX-2 were determined by real-time polymerase chain …