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Glutathione Redox Dynamics And Expression Of Glutathione-Related Genes In The Developing Embryo, Alicia R. Timme-Laragy, Jared V. Goldstone, Barry R. Imhoff, John J. Stegemana, Mark E. Hahn, Jason M. Hansen

Alicia R. Timme-Laragy

Embryonic development involves dramatic changes in cell proliferation and differentiation that must be highly coordinated and tightly regulated. Cellular redox balance is critical for cell fate decisions, but it is susceptible to disruption by endogenous and exogenous sources of oxidative stress. The most abundant endogenous non-protein antioxidant defense molecule is the tri-peptide glutathione (γ-glutamyl-cysteinylglycine, GSH), but the ontogeny of GSH concentration and redox state during early life stages is poorly understood. Here, we describe the GSH redox dynamics during embryonic and early larval development (0–5 days post-fertilization) in the zebrafish (Danio rerio), a model vertebrate embryo. We measured reduced and …